Crystallographic and NMR studies on sterols from green alga Chaetomorpha basiretorsa Setchell

Chemical investigation of the ethanol extract of the marine green alga Chaetomorpha basiretorsa Setchell led to the isolation of a new sterol stigmast-4,28-dien-3α,6β-diol 1 in addition to the five known sterols of β-lawsaritol 2, saringosterol 3, 24-hydroperoxy-24-vinyl - cholesterol 4, β-stigmasterol 5, 29-hydroxystigmasta-5, 24 （28） -dien-3β-ol 6. Compounds were isolated by normal phase silica gel and Sephadex LH - 20 gel colum chromatography, reverse phase HPLC and recrystalization. Their structures were elucidated by spectroscopic methods including MS, IR 1D/2D NMR and X-ray analysis. Cytotoxicity of compounds was screened by using the standard MTF method. All these compounds were isolated from the green alga Chaetomorpha basiretorsa Setchell for the first time and they were inactive （50% inhibitory concentration was greater than 10 μg/cm^3） against KB, Bel -7402, PC - 3M, Ketr 3 and MCF-7 cell lines.     

桂林洞穴滴水对应CaCO3沉积物的稳定同位素特征与环境意义

桂林地区降水及茅茅头大岩滴水滴速和现代碳酸盐的碳氧同位素研究表明:(1)滴水沉积的δ18O达到了同位素沉积平衡,洞穴滴水的δ18O与同期降雨的δ18O值呈正相关关系;(2)在旱季和湿季交替时,滴水δ18O值滞后于降雨δ18O,受活塞效应的影响;(3)δ13C难以达到同位素沉积平衡,主要受滴水动力学影响;(4)同一洞穴不同滴水对环境的响应不同,如果洞穴顶板覆盖较厚,水分在土壤和岩层中滞留时间较长,滴水则反映受到平滑作用的降雨,反之则波动较大.     

支持向量机在海洋随机资料分析中的应用

在随机海洋资料的分析中,需要从大量的实际数据中提取出有用的信息来建立或检验各种海洋理论模型,因此我们要正确运用适当的手段来分析处理各种实际数据。文中简要介绍了数据挖掘中一项新的技术支持向量机,着重介绍了最优化问题的求解算法,以及如何应用于海洋资料的分析处理。     

基于基础地理信息数据库的制图设计与实现

辽宁省测绘局于2007年开始正式启动辽宁省基础地理信息数据库建设工程,该建设工程的目标之一是实现了图库一体化功能.本文详细介绍了基于辽宁省基础地理信息数据库的制图设计与实现.     

Post-depositional modification of NO-3 in snow layers at EastAntarctica and at the headwater of ürumqi River

Acidic species, such as Nitrate, in polar snow and firn layers are “reversibly” deposited, and are sufficiently volatile to undergo significant postdepositional exchange between snow/firn and the atmosphere. Through comparison of the snowpit and snowpack nitrate concentrations from central East Antarctica and the headwater of ürumqi River, we conclude that the nitrate peaks in the uppermost surface snow layers in central Antarctica are not related to an atmospheric signal and must account for post-depositional effects. Such effects, however, are not found in the surface snowpack nitrate profiles from the headwater of ürumqi River. Two reasons may account for the post-depositional difference. At first, nitrate in the polar snow and firn layers appears to be hydrated ion, which can be taken up by the atmosphere, while at the headwater of ürumqi River it seems mainly as mineral ion, which assembles the behavior of aerosol-derived species that are “irreversibly” deposited and do not undergo significant post-depositional exchange with the atmosphere. Secondly, the chemical features of the snow and ice on the Antarctica are mainly determined by wet deposition, to the contrary, dry deposition is more significant at the headwater of lUrumqi River than that on the East Antarctic Plateau.     

Predictability of Chinese Summer Extreme Rainfall Based on Arctic Sea Ice and Tropical Sea Surface Temperature

Chinese summer extreme rainfall often brings huge economic losses, so the prediction of summer extreme rainfall is necessary. This study focuses on the predictability of the leading mode of Chinese summer extreme rainfall from empirical orthogonal function(EOF) analysis. The predictors used in this study are Arctic sea ice concentration(ASIC) and regional sea surface temperature(SST) in selected optimal time periods. The most important role that Arctic sea ice(ASI) plays in the appearance of EOF1 may be strengthening the high pressure over North China, thereby preventing water vapor from going north. The contribution of SST is mainly at low latitudes and characterized by a significant cyclone anomaly over South China. The forecast models using predictor ASIC(PA), SST(PS), and the two together(PAS) are established by using data from 1980 to 2004. An independent forecast is made for the last 11 years(2005-2015). The correlation coefficient(COR) skills between the observed and cross-validation reforecast principal components(PC) of the PA, PS, and PAS models are 0.47, 0.66, and 0.76, respectively. These values indicate that SST is a major cause of Chinese summer extreme rainfall during 1980-2004. The COR skill of the PA model during the independent forecast period of 2004-2015 is 0.7, which is significantly higher than those of the PS and PAS models. Thus, the main factor influencing Chinese summer extreme rainfall in recent years has changed from low latitudes to high latitudes. The impact of ASI on Chinese summer extreme rainfall is becoming increasingly significant.     

Ecological Worldview as the Central Component of Environmental Concern: Clarifying the Role of the NEP

This study examines whether an ecological worldview—operationalized by the New Ecological Paradigm (NEP) Scale--serves as the source of coherence of Environmental Concern (EC). Using data on four samples from the 1992 Gallup “Health of the Planet” Survey and the 2003 and 2010 Chinese General Social Surveys, we found that in all samples not only is the NEP the most powerful predictor of EC, but it also mediates the effects of socio-demographic variables on EC as hypothesized. The results confirm that the NEP is the source of coherence of EC, making it a meaningful construct.     

环莫干山民宿的时空分布特征与成因

民宿是中国乡村振兴和旅游转型升级过程中涌现出的新兴业态和空间利用方式,但学术关注滞后。环莫干山地区是目前中国最典型的民宿发展集聚区之一。本研究通过官方统计资料和实地调研获取环莫干山民宿空间发展数据,构建了ArcGIS和地理探测器结合的民宿空间过程分析方法,基于目的地的微观尺度系统地探讨环莫干山民宿时空分布特征和成因。研究发现：① 2011—2017年环莫干山民宿呈现出逐年增长的态势,空间分布范围不断扩大,空间发展方向出现较大变化,逐渐趋向镇域均衡性发展。目前已经在莫干山风景区毗邻村域形成以“后坞-仙潭-燎原-劳岭-兰树坑”为中心的环莫干山面状核心集聚区和边缘村域多点集聚的民宿空间发展结构。不同档次民宿的时空发展趋势具有一定差异。② 环莫干山民宿的空间过程由集聚到强烈集聚,不同档次民宿的集聚程度均呈现出快速提升的趋势。空间发展过程中早期民宿集聚点扩散效应显著。空间集聚过程与地形地势、核心风景区、交通干线和居民点密切相关。③ 风景景观、发展基础、社会因素和区位因素是环莫干山民宿景观格局异质性的重要决定因素维度,但不同等级民宿的空间决定因素维度和指标差异性显著,两因素维度交互作用对于民宿的空间影响力更为显著。研究对于丰富接待业空间选址的类型研究,总结中国典型民宿集聚区空间发展模式具有重要理论意义,同时对于民宿产业选址和民宿集聚区规划实践具有一定现实意义。     

Selective feeding of bay scallop Argopecten irradians on phytoplankton community revealed by HPLC analysis of phytopigments in Bohai Sea,China

Understanding the feeding selectivity on phytoplankton by shellfish is currently a big challenge.In order to investigate the feeding behavior of bay scallop(Argopecten irradians)on phytoplankton,we compared its compositions of phytopigments in digestive glands with those in the surrounding seawater,and conducted five consecutive investigations between July and November 2016 in a bay scallop culture area along coast of Qinghuangdao City,northwest of the Bohai Sea,China.Phytopigments in four-size fractionated phytoplankton of seawater(micro-(20-200 μm);nano(L)-[10-20 μm];nano(S)-[2.7-10 μm],and pico-[2.7 μm])and digestive glands of A.irradians were examined to investigate the selective feeding of A.irradians.Results show that fucoxanthin and peridinin constituted the major part of taxonomically diagnostic carotenoids(TDCs)in the micro-and nano(L)-phytoplankton in seawater.Compared with total phytoplankton biomass of seawater(TPB,sum of the four sizes),a substantial decrease of fucoxanthin proportion to total DCs in digestive glands was observed while that of peridinin,19'-butanoyloxyfucoxanthin,alloxanthin and 19'-hexanoyloxy-fucoxanthin showed an obvious increase when those pigments were mainly confined to micro-sized phytoplankton(20-200 μm).However,zeaxanthin and prasinoxanthin were mainly confined to nano(s)-and pico-phytoplankton,of which the proportions in digestive glands were usually lower in TPB.The contribution of lutein to total DCs in digestive glands(with an average of 7.23%)increased compared with TPB of seawater(with an average of 0.63%)during all five sampling times.     

Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq,and cross-amplification to related species

Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( H_o ) and expected heterozygosity ( H_e ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.