Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach

Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.     

The Effect of Human Exclusion on the Population Structure of Key-Hole Limpets Fissurella crassa and F. limbata on the Coast of Central Chile

Abstract. We describe the basic ecology of two key-hole limpets, Fissurella crassa and Fissurella limbata , which are heavily harvested by coastal shellfishermen (mariscadores) at the rocky intertidal of central Chile. The ecological role of human predation on these species was assessed. Anthropic exclusion from a stretch of the rocky intertidal at Las Cruces (non-harvested area) for about two and a half years resulted in changes of the densities and size distributions of these fissurelid species as compared to populations in control zones (harvested area). Differential human predation generates, on key-hole limpets, responses of different intensities related to the accessibility of man to the mesohabitats (sheltered platforms, exposed platforms and vertical walls) and fringes of the intertidal (mid-low intertidal and Lessonia nigrescens fringe). Thus, size distributions and mean densities of both fissurelids differ widely between the harvested and non-harvested areas on both platform habitats but not on vertical walls. In the mid-low intertidal fringe, differences are greater than in the less accessible L. nigrescens intertidal fringe. Moreover, we discuss the way in which other species, directly harvested by mariscadores (i. e., the mollusc Concholepas concholepas) can affect the keyhole limpet populations of central Chile rocky shores. Finally, we highlight the ecological role of these limpets on the dynamics of intertidal ecosystems and how they can modify community landscape.     

Stratigraphic framework of gas accumulations in the Ría de Pontevedra (NW Spain)

Knowledge of marine geological environments in which shallow gas is accumulating is becoming increasingly important in global studies of climate change because a measurable proportion of the total methane source comes from continental margins. Previous studies have revealed that coastal environments represent important geological environments where microbial methane is being generated, is accumulating, and is being released. In the Ría de Pontevedra, at least 4.5 km² of seafloor in the innermost part of the ría is underlain by sediments containing natural gas. Seismic interpretation contributes new findings for the definition of periods and geological environments in which the gas could have been generated, and is accumulating and released in the Ría de Pontevedra. Groundtruthing the seismic data (facies, environments) makes it possible to identify favourable geological environments for gas generation in the sedimentary infill of the Ría de Pontevedra. Sequence stratigraphy based on high-resolution seismic profiles and post-Last Glacial Maximum sea-level records makes it possible to establish the stratigraphic architecture of the ría and to define the periods in which gas could have been generated. The results of this study show that the sedimentary infill is composed of a fifth-order sequence developed since the Last Glacial Maximum. Within this sequence, gas appears to have accumulated in the Holocene deposits associated with the latest transgressive and highstand system tracts. Seismic analysis shows that gas could have been generated in different geological environments in the Ría de Pontevedra. If coastal environments at times of lower sea level were similar to those of the present, organic-rich mud deposits (deposited mainly in lakes, estuaries and floodplains) could have survived transgression and remained buried as potential gas sources in the inner part of the ría.     

Effects of crab–halophytic plant interactions on creek growth in a S.W. Atlantic salt marsh: A Cellular Automata model

The Bahía Blanca Estuary (38° 50′ S, and 62° 30′ W) presents salt marshes where interactions between the local flora (Sarcocornia perennis) and fauna (Chasmagnathus granulatus) generate some kind of salt pans that alter the normal water circulation and condition its flow and course towards tidal creeks. The crab–vegetation dynamics in the salt marsh presents variations that cannot be quantified in a reasonable period of time. The interaction between S. perennis plant and C. granulatus crab is based on simple laws, but its result is a complex biological mechanism that causes an erosive process on the salt marsh and favors the formation of tidal creeks. To study it, a Cellular Automata model is proposed, based on the laws deduced from the observation of these phenomena in the field, and then verified with measurable data within macroscale time units. Therefore, the objective of this article is to model how the interaction between C. granulatus and S. perennis modifies the landscape of the salt marsh and influences the path of tidal creeks. The model copies the basic laws that rule the problem based on purely biological factors.The Cellular Automata model proved capable of reproducing the effects of the interaction between plants and crabs in the salt marsh. A study of the water drainage of the basins showed that this interaction does indeed modify the development of tidal creeks. Model dynamics would likewise follow different laws, which would provide a different formula for the probability of patch dilation. The patch shape can be obtained changing the pattern that dilates.     

The mysid Siriella armata as a test organism in toxicology: Effects of cadmium

A shrimp-like crustacean of the coastal Zooplankton in the north-western Mediterranean Sea, the mysid Siriella armata, offers a test organism whose life cycle is entirely controllable under laboratory conditions. The effects of the heavy metal cadmium were studied on adults and juveniles cultured in the laboratory including acute toxicity, bioaccumulation and effects on growth, ingestion and moult cycle.     

The University of Durham Southern Hemisphere VHE gamma ray telescope

The design of very high energy (VHE) gamma ray telescopes is discussed and it is shown that a triple-mirror telescope operating in threefold fast coincidence provides an effective instrument. The design, construction and operation of a large VHE gamma ray telescope with an energy threshold of 300 GeV (at the zenith) and incorporating several novel features is described. The use of a coincidence guard ring technique is shown to result in the rejection of about 50% of the off-source (proton) events which form a high background and limit the sensitivity of such telescopes.     

Spin rate estimation of sounding rockets using GPS wind-up

Carrier phase wind-up is a well-known effect that arises from the relative rotation between a transmitting and receiving antenna. In GPS measurements at L1 frequency, this effect translates into an error of 19.029 cm per full relative rotation of antennas. Since this effect is independent of the satellite elevation for pure rotation about the antenna boresight axis, it is usually absorbed by the clock estimation in navigation algorithms. Therefore, the impact of wind-up is usually neglected for applications that do not require accuracies to the cm level like RTK. However, in receiving platforms with high rotation rate, the accumulated wind-up value can be important and actually be larger than receiver noise or even ionospheric variations. Therefore, in such scenarios, the wind-up contribution can be isolated and used as a source of information to compute the spin rate of such platforms using an appropriate combination of GPS observables. This work shows some results of a coarse, yet simple, approach to monitor the rotation angle and spin-rate of spin stabilized sounding rockets flown by DLR.     

Spatial Heterogeneity Created by Burrowing Crabs Affects Human Impact on Migratory Shorebirds

We show that, as previously described, there are conflicts between areas used by people and shorebirds. However, we propose that burrowing organisms add complexity to the shorebird–human interaction that should be taken into account for management and conservation. This is because SW Atlantic mudflats are dominated by high densities (up to 60 crabs m⁻²) of a strong bioturbator, the burrowing crab Neohelice granulata (=Chasmagnathus granulatus). These crabs affect the habitat use and foraging performance of shorebird species. The two-banded plover Charadrius falklandicus feed more in burrow areas whereas the yellowlegs Tringa spp. and the white-rumped sandpiper Calidris fuscicollis tend to avoid burrow areas. We evaluated the effect of human recreational activities on shorebirds, taking into account shorebird–crab interaction, during shorebird migratory periods (November to April) of 2001, 2002, and 2003. This study was performed at the Mar Chiquita coastal lagoon (37° 40′ S, Argentina). Results showed that people only rarely used the extensive soft bottom intertidals dominated by burrowing assemblages of N. granulata because the soft sediments make walking difficult. Therefore, human impact is reduced for the two-banded plover. However, for several migratory shorebirds that also avoid burrow areas, these areas act as suboptimal alternative areas when human impact is important in their preferred habitats. When human activity is low, shorebirds remain foraging in the area but they decrease their feeding intakes. The extensions of burrow areas differ among estuaries and are likely to generate between-estuaries differences as stopover sites for shorebirds. Also, other species that form large burrowing assemblages can have a similar impact on shorebird–human interactions.     

Simulations of 3D DC borehole resistivity measurements with a goal-oriented <Emphasis Type="Italic">hp</Emphasis> finite-element method. Part II: through-casing resistivity instruments

We simulate direct current (DC) borehole resistivity measurements acquired in steel-cased deviated wells for the assessment of rock formation properties. The assumed data acquisition configuration considers one current (emitter) and three voltage (collector) electrodes that are utilized to measure the second difference of the electric potential along the well trajectory. We assume a homogeneous, 1.27-cm-thick steel casing with resistivity equal to 10^− 5 Ω· m. Simulations are performed with two different numerical methodologies. The first one is based on transferring two-dimensional (2D) axisymmetric optimal grids to a three-dimensional (3D) simulation software. The second one automatically produces optimal 3D grids yielded by a 3D self-adaptive goal-oriented algorithm. Both methodologies utilize high-order finite elements (FE) that are specially well-suited for problems with high-contrast coefficients and rapid spatial variations of the electric field, as it occurs in simulations that involve steel-cased wells. The method based on transferring 2D-optimal grids is efficient in terms of CPU time (few seconds per logging position). Unfortunately, it may produce inaccurate 3D simulations in deviated wells, even though the error remains below 1% for the axisymmetric (vertical) well. The method based on optimal 3D grids, although less efficient in terms of CPU time (few hours per logging position), produces more accurate results that are validated by a built-in a posteriori error estimator. This paper provides the first existing simulations of through-casing resistivity measurements in deviated wells. Simulated resistivity measurements indicate that, for a 30° deviated well, measurements in conductive layers 0.01 Ω· m) are similar to those obtained in vertical wells. However, in resistive layers (10,000 Ω· m), we observe 100% larger readings in the 30° deviated well. This difference becomes 3,000% for the case of a 60° deviated well. For this highly-deviated well, readings corresponding to the conductive formation layer are about 30% smaller in magnitude than those in a vertical well. Shoulder effects significantly vary in deviated wells.     

Water balance in the playa-lakes of an arid environment,Monegros, NE Spain

The playa-lakes of the Monegros desert in north-east Spain are saline wetlands in an arid environment, a rare phenomenon in Europe. These extremely valuable habitats are threatened by changes associated with agricultural expansion and incorporation of new irrigated areas. An understanding of the present hydrologic regime will enable changes to be identified, particularly those brought about by flooding and pollution caused by irrigation surplus. This study sets out to show the results of applying a daily water balance in three selected playa-lakes. The balance was in two parts and consisted of: (1) the average balance for all the endorheic basin using the BALAN_11 program, and (2) the water balance in some playa-lakes, applying discharge flows obtained from the previous balance. The resulting volumes of water were converted to water depths and contrasted with reference volumes taken from field and Landsat images. The model was calibrated by applying various hypotheses of function which enabled the results to be adjusted. The proposed balance is an acceptable reproduction of field water measurements during this period, and underlines the consistency of the conceptual model. The methodology used is appropriate for understanding the playa-lakes function and for monitoring them for conservation purposes.