黑鲷(Acanthopagrus schlegeli)肿瘤坏死因子α cDNA的克隆及特征分析

采用同源克隆的方法，从黑鲷中获得肿瘤坏死因子(TNFα)全长cDNA序列。该序列含1288个核苷酸，可编码253个氨基酸的TNF前体蛋白。它是一种跨膜蛋白，无糖基化位点和信号肽结构。黑鲷TNFα与其它鱼类的TNFα的相似性很高，占据进化上独立的分支；与哺乳类TNFα和TNFG源于共同的祖先。基因结构分析显示，该基因含有TNF家族pmfile和TNF家族的标签序列；在参与TNFα基因二硫键形成的两个半胱氨酸和TNFα三聚体形成的位点高度保守；空间结构模拟显示，它与哺乳类TNFα的空间结构相似，都是由两个β折叠片组成，每个折叠片包含5个反向平行的β链。表达研究结果表明，黑鲷TNFα在检测的各个组织中均为组成型表达，表现为在刺激与非刺激鱼体中，都可以检测到黑鲷TNFα的表达，但是其表达水平在不同组织中有很大差异。黑鲷TNFα在头肾、脾脏和鳃的表达量较高，而在心脏、肝脏、血液、肾脏和大脑中表达量较低。     

前苏联七十年海冰研究回顾

本文简要介绍了前苏联近七十年来海冰研究的主要成果及其采用的一些预报方法。     

大黄鱼病原副溶血弧菌单克隆抗体制备及其应用

用甲醛灭活副溶血弧菌(Vibrio parahaemolyticus)和溶藻弧菌(V.alginolyticus)制成免疫原免疫BALB/c小鼠,利用淋巴细胞杂交瘤技术,获得1株特异的针对副溶血弧菌的单克隆抗体,命名为D6F3H5。腹水及培养上清液抗体的ELISA效价分别为:1∶5 120和1∶1 280,该单克隆抗体与其它细菌没有明显的交叉反应。利用该单克隆抗体和兔抗副溶血弧菌多克隆抗体,建立了检测副溶血弧菌的双抗体夹心ELISA方法。该方法对副溶血弧菌的最小检出量为5×104个/mL。用双抗体夹心ELISA检测大黄鱼(Pseudosciaena crocea)样品,14尾患病大黄鱼中有11尾检出副溶血弧菌,而10尾健康大黄鱼都没有检出副溶血弧菌。由此可见,本试验制备的高特异性的抗副溶血弧菌单克隆抗体,可以用于患病大黄鱼副溶血弧菌的快速诊断。     

On the Line Formation in Stellar Magnetized Atmospheres

1 INTRODUCTIONIn the previous paper (on et al. 1999, hereafter Paper I), we investigated the wavelength-dependence of four colltribution functions (CFs) derived from dmerent formal solutions andreferring to different emergellt quantities in the unpolarized case. Because one cannot generallyassign a single formation region to the whole line band in a real stellar atmosphere, e.g., the solaratmosphere, instead, the line formation region can be defined as the layers deviating farthestfrom t…     

The causality between the rapid rotation of a sunspot and an X3.4 flare

Using multi-wavelength data of Hinode, the rapid rotation of a sunspot in ac-tive region NOAA 10930 is studied in detail. We found extraordinary counterclockwise rotation of the sunspot with positive polarity before an X3.4 flare. From a series of vector magnetograms, it is found that magnetic force lines are highly sheared along the neu-tral line accompanying the sunspot rotation. Furthermore, it is also found that sheared loops and an inverse S-shaped magnetic loop in the corona formed gradually after the sunspot rotation. The X3.4 flare can be reasonably regarded as a result of this movement. A detailed analysis provides evidence that sunspot rotation leads to magnetic field linestwisting in the photosphere. The twist is then transported into the corona and triggers flares.     

ENSO indices from sea surface salinity observed by Aquarius and Argo

Analysis of the first 26 months of data from the Aquarius satellite confirms the existence of a sharp sea surface salinity (SSS) front along the equator in the western equatorial Pacific. Following several earlier studies, we use the longitudinal location of the 34.8-psu isohaline as an index, termed Niño-S34.8, to measure the zonal displacement of the SSS front and consequently the eastern edge of the western Pacific warm pool. The on-going collection of the Array for Real-time Geostrophic Oceanography (ARGO) program data shows high correlations between Niño-S34.8 and the existing indices of El Niño, suggesting its potential important role in ENSO evolution. Further analysis of the ARGO data reveals that SSS variability in the southeastern tropical Pacific is crucial to identify the type of El Niño. A new SSS index, termed the southeastern Pacific SSS index (SEPSI), is defined based on the SSS variability in the region (0°–10°S, 150°–90°W). The SEPSI is highly correlated with the El Niño Modoki index, as well as the Trans-Niño index, introduced by previous studies. It has large positive anomalies during central Pacific El Niño or El Niño Modoki events, as a result of enhanced zonal sea surface temperature gradients between the central and eastern tropical Pacific, and can be used to characterize the type of El Niño. The processes that possibly control these SSS indices are also discussed.     

渤黄海沿海2月份海平面异常偏高成因分析

使用1980—2010年的水位、气温、海温、气压和风场资料,对中国渤黄海沿海2月份的海平面变化特征以及异常偏高成因进行了探讨,分析结果表明:近30年,渤黄海沿海2月份海平面呈现明显的上升趋势,2009年和2010年2月份的海平面达到近30年的高值,冬季高海平面导致全年平均海平面偏高。近两年2月份海平面处于多个长周期振动的高位重合期,各振幅叠加的结果近100 mm,对海平面起了明显的抬升作用。高海平面使得辽宁、河北以及山东等沿岸地区的海水入侵距离和土壤盐渍化程度均有所增加,海岸侵蚀加重;上海在2009—2010年连续2年2月份发生了近年较严重的咸潮入侵。2009年和2010年2月份,气压较常年同期显著偏低,冬季季风显著偏弱,是海平面上升的主要原因之一。     

深水平台吸力沉箱基础工程地质问题分析

针对深水平台吸力沉箱基础,讨论了与沉箱安装有关的分析方法及涉及的工程地质参数,分析了承受竖向拉拔荷载、倾斜与水平荷载作用的吸力沉箱极限承载力的分析方法及涉及的工程地质参数,对与吸力沉箱设计有关的其它问题也进行了分析.在此基础上,阐明了与吸力沉箱设计有关的工程场地调查内容及需要确定的工程地质参数.其目的是为开发深水平台吸...     

Geochemical evidence of an extraterrestrial component in impact melt breccia from the Paleoproterozoic Dhala impact structure,India

The Paleoproterozoic Dhala structure with an estimated diameter of ~11 km is a confirmed complex impact structure located in the central Indian state of Madhya Pradesh in predominantly granitic basement (2.65 Ga), in the northwestern part of the Archean Bundelkhand craton. The target lithology is granitic in composition but includes a variety of meta‐supracrustal rock types. The impactites and target rocks are overlain by ~1.7 Ga sediments of the Dhala Group and the Vindhyan Supergroup. The area was cored in more than 70 locations and the subsurface lithology shows pseudotachylitic breccia, impact melt breccia, suevite, lithic breccias, and postimpact sediments. Despite extensive erosion, the Dhala structure is well preserved and displays nearly all the diagnostic microscopic shock metamorphic features. This study is aimed at identifying the presence of an impactor component in impact melt rock by analyzing the siderophile element concentrations and rhenium‐osmium isotopic compositions of four samples of impactites (three melt breccias and one lithic breccia) and two samples of target rock (a biotite granite and a mafic intrusive rock). The impact melt breccias are of granitic composition. In some samples, the siderophile elements and HREE enrichment observed are comparable to the target rock abundances. The Cr versus Ir concentrations indicate the probable admixture of approximately 0.3 wt.% of an extraterrestrial component to the impact melt breccia. The Re and Os abundances and the ¹⁸⁷Os/¹⁸⁸Os ratio of 0.133 of one melt breccia specimen confirm the presence of an extraterrestrial component, although the impactor type characterization still remains inconclusive.