印尼热泉菌Bacillus sp. BI-3产琼胶酶的发酵条件优化

采用响应面法对1株高产琼胶酶的印度尼西亚热泉菌Bacillus sp. BI-3的发酵条件进行优化。首先以温度、pH、不同碳源、不同氮源、不同金属离子及接种量作为唯一变量进行单因素实验, 筛选出对酶活有显著影响的单因素取值范围; 参考单因素实验结果, 采用Plackett-Burman实验设计筛选出影响酶活主要因素, 再利用Box-Behnken设计及响应面分析法进行回归分析以确定最佳发酵条件。结果表明, D-甘露糖、氯化锶和氯化钙与酶活存在着显著的相关性, 通过求解回归方程得到Bacillus sp. BI-3的发酵条件系统优化的结果为: 酵母粉0.3%、D-甘露糖0.66%、蛋白胨0.6%、氯化钙7.21mmol/L、氯化锶6.00mmol/L、氯化钠添加量6‰、培养温度为55℃、接种量1%、初始pH 6.0, 优化后发酵上清液的酶活达到6.0U/mL, 比优化前提高了2.4倍。     

“Digital earth” in support of an online oceanic educational public service and popularization

According to the initial vision of "digital earth" (DE), the public should constitute a significant proportion of its users. However, to date, most of the studies and applications have focused on science, the private sector and government. A DE-supported online oceanic educational public service and popularization system, iOcean, is studied. First, the vision for the public’s engagement with "digital ocean" is described: an analysis is presented from four aspects, i.e., the space dimension, the time dimension, the state dimension and its relationship with human beings. Second, the technical framework of iOcean is discussed, including data updating and model computing, the data, the function, and the application layers. Third, two key technologies are studied in detail that will enable the construction of iOcean. More than half a million public viewers have used the current version’s website. Practical demonstrations show that iOcean can bring virtual oceans to web browsers and desktops and construct a bridge between government departments and the general public.     

面向原位数据的海洋观测客户端软件设计与实现

原位数据在海洋观测过程中具有重要作用。本文针对面向原位数据的海洋观测客户端软件开发复用率低、不同观测平台之间数据通用性差的问题,结合已有的海洋观测软件,利用ActiveX控件开发技术、WebGIS以及WebService技术,提出面向原位数据的海洋观测客户端软件设计模型,通过原型系统验证了模型设计的有效性。该设计模型对于面向原位数据的海洋观测客户端软件的高效率开发具有重要作用。     

响应面法优化南极菌Psychrobacter sp.B-3的发酵产糖条件

为对南极菌Psychrobacter sp.B-3产胞外糖的发酵条件进行优化,以温度、pH、不同碳源、不同氮源、不同金属离子及海水配比作为唯一变量进行单因素实验,筛选出对产糖有显著影响的单因素取值范围;在此基础上,利用Plackett-Burman实验设计筛选出影响产多糖的3个主要因素,即酵母粉、陈海水配比和装液量.然后通过Box-Behnken实验设计及响应面分析法进行回归分析,得出了南极菌Psychrobacter sp.B-3发酵条件系统优化的结果为:酵母粉1.1 g/L,陈海水与自来水的配比为2:1,装液量为50％,蛋白胨6.0 g/L,接种量1.5％,培养时间64 h,BaCl20.03 g/L,温度10℃,转速150 r/min,pH为7.0.优化后发酵液中的糖含量由优化前的444 μg/mL提高到624 μg/mL,相比初始糖产量提高了40％.结果表明,响应面法可显著优化南极菌Psychrobacter sp.B-3液体发酵产胞外糖的条件.     

基于SOA的数字城市公共平台设计与实现

针对城市信息化建设中存在的建设周期长、开发成本高、运行维护困难等问题,本文从软件架构、系统功能、服务性能等方面进行研究,设计并开发基于SOA的数字城市公共平台。平台采用松散耦合的WebSer-vices、Ajax等技术,结合数据资源控制访问、平台架构分离等策略,实现对多源异构空间数据的分布式集中管理和授权共享,封装形成统一的服务接口及应用框架,辅助业务系统的快速搭建与无缝集成;并在二维GIS基础上扩展添加三维GIS服务。     

中国东北地区晚二叠世岩相古地理特征

在6条野外实测剖面和38条收集剖面的基础上, 通过剖面的岩石组合特征及样品的元素地球化学分析, 对中国东北地区晚二叠世的沉积环境和古地理特征进行了研究。结果显示, 东北地区上二叠统样品常量元素具有高Si、Al, 低P、Mn、Ca的特点;微量元素具有高V、Rb、Zr、Ba, 低Be、Mo、Cd、Ta和U的特点;稀土元素表现为轻、重稀土分异显著, 轻稀土相对富集, Eu负异常的特征。沉积环境主体为陆相淡水还原环境;存在北部、东部和西部3个物源, 其中北部和东部为主要物源方向;发育三角洲相、扇三角洲相和湖泊相沉积。此外, 林西组底部样品在常量、微量及稀土元素的异常和杨家沟组底部大量碳酸盐岩砾石的出现, 说明晚二叠世初期局部有海侵发生。中国东北地区晚二叠世沉积初期为海陆交互环境, 主体为陆相湖泊沉积环境。     

Engineering geological assessment for route selection of railway line in geologically active area: A case study in China

The Cheng-Lan railway links Chengdu, a central city in Southwestern China, and Lanzhou, a central city in Northwestern China. The railway passes through the Longmenshan fault zone (Wenchuan earthquake happened there on May 12, 2008), Minjiang fault zone, and Dongkunlun fault zone, which are all active. It runs over the Yangtze River and the Yellow River, and crosses high mountains and deep valleys. There exists, along the railway’s alignment, different kinds of strata of hard granite and soft, weak metamorphic rocks such as carbonaceous slate, schist, and phyllite. It is, therefore, a key issue for such an infrastructure construction to assess the engineering geological conditions and risks, so as to mitigate or avoid possible georisks and to offer optional designs. Geological survey and georisk assessment along the railway corridor are carried out. Special attention is given to active faults, earthquakes and seismic zones. Based on these, discussions about geological aspects for route selection of the railway are conducted and countermeasures for georisk control are proposed accordingly. Main conclusions are achieved as follows: (1) Geohazards such as landslides, rockfalls and debries flows dominate both the route selection of the railway and the engineering structures (e.g., tunnels or bridges) adopted; (2) Tunnel has been proved to be an excellent structure for linear engineering in geologically active area; and (3) In the case where avoiding is impractical, necessary protection measures should be taken to engineering slopes in high earthquake intensity areas, especially the area with earthquake of Ms. 8 or greater.     

风光发电系统在无人值守地震台站的设计

风光互补发电技术在边防、通信、公路等已得到广泛应用,能否将这一绿色、清洁、可再生能源应用于地震无人值守台站,山东省在龙口地震台已做先期试点,通过一年试运行,风光互补发电系统能够满足地震无人值守台站的工作要求。     

山东区域地电场观测质量分析

对7个台的观测数据质量进行评价和分析,结果表明:郯城、菏泽、大山和陵阳地震台数据质量较好,精度较高,可为地震研究提供可靠的数据资料.     

渤黄海沿海2月份海平面异常偏高成因分析

使用1980—2010年的水位、气温、海温、气压和风场资料,对中国渤黄海沿海2月份的海平面变化特征以及异常偏高成因进行了探讨,分析结果表明:近30年,渤黄海沿海2月份海平面呈现明显的上升趋势,2009年和2010年2月份的海平面达到近30年的高值,冬季高海平面导致全年平均海平面偏高。近两年2月份海平面处于多个长周期振动的高位重合期,各振幅叠加的结果近100 mm,对海平面起了明显的抬升作用。高海平面使得辽宁、河北以及山东等沿岸地区的海水入侵距离和土壤盐渍化程度均有所增加,海岸侵蚀加重;上海在2009—2010年连续2年2月份发生了近年较严重的咸潮入侵。2009年和2010年2月份,气压较常年同期显著偏低,冬季季风显著偏弱,是海平面上升的主要原因之一。