Enzymatic microbial Mn(II) oxidation and Mn biooxide production in the Guaymas Basin deep-sea hydrothermal plume

Microorganisms play important roles in mediating biogeochemical reactions in deep-sea hydrothermal plumes, but little is known regarding the mechanisms that underpin these transformations. At Guaymas Basin (GB) in the Gulf of California, hydrothermal vents inject fluids laden with dissolved Mn(II) (dMn) into the deep waters of the basin where it is oxidized and precipitated as particulate Mn(III/IV) oxides, forming turbid hydrothermal “clouds”. Previous studies have predicted extremely short residence times for dMn at GB and suggested they are the result of microbially-mediated Mn(II) oxidation and precipitation. Here we present biogeochemical results that support a central role for microorganisms in driving Mn(II) oxidation in the GB hydrothermal plume, with enzymes being the primary catalytic agent. dMn removal rates at GB are remarkably fast for a deep-sea hydrothermal plume (up to 2 nM/h). These rapid rates were only observed within the plume, not in background deep-sea water above the GB plume or at GB plume depths (∼1750-2000 m) in the neighboring Carmen Basin, where there is no known venting. dMn removal is dramatically inhibited under anoxic conditions and by the presence of the biological poison, sodium azide. A conspicuous temperature optimum of dMn removal rates (∼40 °C) and a saturation-like (i.e. Michaelis-Menten) response to O₂ concentration were observed, indicating an enzymatic mechanism. dMn removal was resistant to heat treatment used to select for spore-forming organisms, but very sensitive to low concentrations of added Cu, a cofactor required by the putative Mn(II)-oxidizing enzyme. Extended X-ray absorption fine structure spectroscopy (EXAFS) and synchrotron radiation-based X-ray diffraction (SR-XRD) revealed the Mn oxides to have a hexagonal birnessite or δ-MnO₂-like mineral structure, indicating that these freshly formed deep-sea Mn oxides are strikingly similar to primary biogenic Mn oxides produced by laboratory cultures of bacteria. Overall, these results reveal a vigorous Mn biogeochemical cycle in the GB hydrothermal plume, where a distinct microbial community enzymatically catalyzes rapid Mn(II) oxidation and the production of Mn biooxides.     

Maturation related changes in the distribution of ester bound fatty acids and alcohols in a coal series from the New Zealand Coal Band covering diagenetic to catagenetic coalification levels

A rank series of lignites and coals of low to moderate maturation levels (vitrinite reflectance (R₀): 0.27–0.8%) from the New Zealand Coal Band were investigated using alkaline ester cleavage experiments to reveal compositional changes of ester bound components (fatty acids and alcohols) during increasing maturation. Ester bound alcohols are found to be present in highest amounts in the very immature lignite samples (R₀: 0.27–0.29%), but show a rapid decrease during early diagenesis. Ester bound fatty acids also show an initial exponential decrease during diagenesis, but reveal an intermittent increase during early catagenesis before decreasing again during main catagenesis. This intermittent increase was related to the short chain fatty acids. To obtain a maturity related signal and to eliminate facies related scattering in the amounts of fatty acids in the coal samples, the carbon preference index of fatty acids (CPI_FA) parameter is introduced here. For the long chain fatty acids (C₂₀–C₃₂) originating from terrigenous plant debris, the CPI_FA decreases with increasing maturity, showing a strong maturation related signal. During diagenesis, the same trend can be observed for the short chain fatty acids, but the intermittent increase in the amounts of short chain fatty acids is also accompanied by high CPI_FA values. This indicates less altered organic biomass at this advanced maturation level and is in contrast to the mature CPI_FA signal of the long chain fatty acids of the same samples. One possible reason for this discrepancy could be extremely different amounts of short and long chain fatty acids in the original source organic matter of these samples. However, another intriguing explanation could be the incorporation of immature bacterial biomass from deep microbial communities containing C₁₆ and C₁₈ fatty acids as main cell membrane components. Deep microbial life might be stimulated at this interval by the increasing release of thermally generated potential substrates from the organic matrix during early catagenesis. In contrast to the fatty acids, the high amounts of alcohols in the immature lignite samples are also visible in the alkene distribution from the open system pyrolysis experiments of the organic matrix before and after saponification.     

A novel procedure to detect low molecular weight compounds released by alkaline ester cleavage from low maturity coals to assess its feedstock potential for deep microbial life

In recent years the widespread occurrence of microorganisms was demonstrated in deep marine and terrestrial sediments. With this discovery inevitably the question of the potential carbon and energy sources for this deep subsurface microbial life arises. In the current study a new method for the investigation of low molecular weight (LMW) organic acids linked to the kerogen matrix is presented. These LMW organic acids form a potential feedstock for deep microbial populations. Twelve coal samples of different maturity (vitrinite reflectance (R₀) of 0.28–0.80%) from several coal mines on the North and South Island of New Zealand (NZ) were examined to assess the amount of bound LMW organic acids. Formate, acetate and oxalate were detected in significant amounts whereas the amounts of these compounds decrease with increasing maturity of the coal sample. This decrease of LMW organic acids mainly correlates to the phase of diagenetic alteration of the organic matter characterized by the release of oxygen containing compounds. Concomitantly, it coincides with temperature conditions assumed to be still compatible with microbial life in the deep subsurface. First assessments of the feedstock potential and generation rates of LMW organic acids indicate that the NZ coals investigated exhibit the potential to feed deep terrestrial microbial life with appropriate substrates over geological time spans.     

Latest Pleistocene and Holocene glacier fluctuations in western Canada

We summarize evidence of the latest Pleistocene and Holocene glacier fluctuations in the Canadian Cordillera. Our review focuses primarily on studies completed after 1988, when the first comprehensive review of such evidence was published. The Cordilleran ice sheet reached its maximum extent about 16 ka and then rapidly decayed. Some lobes of the ice sheet, valley glaciers, and cirque glaciers advanced one or more times between 15 and 11 ka. By 11 ka, or soon thereafter, glacier cover in the Cordillera was no more extensive than at the end of the 20th century. Glaciers were least extensive between 11 and 7 ka. A general expansion of glaciers began as early as 8.4 ka when glaciers overrode forests in the southern Coast Mountains; it culminated with the climactic advances of the Little Ice Age. Holocene glacier expansion was not continuous, but rather was punctuated by advances and retreats on a variety of timescales. Radiocarbon ages of wood collected from glacier forefields reveal six major periods of glacier advance: 8.59–8.18, 7.36–6.45, 4.40–3.97, 3.54–2.77, 1.71–1.30 ka, and the past millennium. Tree-ring and lichenometric dating shows that glaciers began their Little Ice Age advances as early as the 11th century and reached their maximum Holocene positions during the early 18th or mid-19th century. Our data confirm a previously suggested pattern of episodic but successively greater Holocene glacier expansion from the early Holocene to the climactic advances of the Little Ice Age, presumably driven by decreasing summer insolation throughout the Holocene. Proxy climate records indicate that glaciers advanced during the Little Ice Age in response to cold conditions that coincided with times of sunspot minima. Priority research required to further advance our understanding of late Pleistocene and Holocene glaciation in western Canada includes constraining the age of late Pleistocene moraines in northern British Columbia and Yukon Territory, expanding the use of cosmogenic surface exposure dating techniques, using multi-proxy paleoclimate approaches, and directing more of the research effort to the northern Canadian Cordillera.     

Fifty years of organisational change in container shipping: regional shift and the role of family firms

Spectacular growth has marked the industry initiated by Malcolm McLean with the sailing of the Ideal-X in 1956. While the growth of container shipping has been typically seen in terms of technological advances, increasing vessel capacity, traffic growth, financial performance and competitiveness, it has been shaped also by organisational transformations. This paper provides an overview of the major companies that make up the container shipping industry, tracing the rapid adoption of containerisation by American carriers to its diffusion to Europe and then Asia. While several carriers belong to business conglomerates, the most dynamic in recent years have been those that are those that possess a family structure. About 12 of the present top 20 carriers are largely family controlled, including 4 out of the top 5. Unlike other capital intensive industries, where the power has shifted towards corporate governance, the container shipping industry retains a strong individualistic entrepreneurial character. At a time when North American ownership in container shipping is no more, the spirit of innovation began 50 years ago by an American visionary is still evident in the entrepreneurial dynamism of many of the industry leaders.     

Genealogical concordance,comparative species delimitation,and the specific status of the Caspian pipefish Syngnathus caspius (Teleostei: Syngnathidae)

To evaluate the specific validity of the Caspian pipefish Syngnathus caspius, we used a comparative molecular species delimitation method on a COI barcode library of Syngnathus, as well as principles of genealogical concordance. Comparative species delimitation allowed us to delineate putative species without a priori assignment of individuals to nominal species, while genealogical concordance extended our species delimitation results to multiple genes, multiple codistributed species, and comparisons with biogeographic evidence. All species delimitation analyses including two topology‐based, one network‐based, and one distance‐based analysis showed genetically isolated lineages of pipefish in the Black and Caspian Sea, corresponding to S. abaster and S. caspius, respectively. Mean evolutionary divergence between the two lineages (0.029) was within the range separating species of Syngnathus (0.024–0.217). The interclade/intraclade ratio of variation was comparable to the operational criterion of divergence between clades greater or equal to 10 × the level within clades to recognize separate species. Our argument on taxonomic validity of S. caspius is also supported by the principles of genealogical concordance as a conceptual basis for recognition of biological species. As a second objective, using a limited number of S. caspius specimens from two semi‐confined water bodies along the Caspian Sea south coastal zone (i.e., Anzali Wetland in the west and Gorgan Bay in the east), we searched for a possible matrilineal structure. The retrieved phylogeographic pattern was characterized by a shallow genealogy and lineage distributions varied, most probably caused by low to modest contemporary gene flow between populations of S. caspius across the southern Caspian Sea that are linked tightly through history.     

The impacts of mountain pine beetle disturbance on the energy balance of snow during the melt period

Mountain snowpacks provide most of the annual discharge of western US rivers, but the future of water resources in the western USA is tenuous, as climatic changes have resulted in earlier spring melts that have exacerbated summer droughts. Compounding changes to the physical environment are biotic disturbances including that of the mountain pine beetle (MPB), which has decimated millions of acres of western North American forests. At the watershed scale, MPB disturbance increases the peak hydrograph, and at the stand scale, the ‘grey’ phase of MPB canopy disturbance decreases canopy snow interception, increases snow albedo, increases net shortwave radiation, and decreases net longwave radiation versus the ‘red’ phase. Fewer studies have been conducted on the red phase of MPB disturbance and in the mixed coniferous stands that may follow MPB‐damaged forests. We measured the energy balance of four snowpacks representing different stages of MPB damage, management, and recovery: a lodgepole pine stand, an MPB‐infested stand in the red phase, a mixed coniferous stand (representing one successional trajectory), and a clear‐cut (representing reactive management) in the Tenderfoot Creek Experimental Forest in Montana, USA. Net longwave radiation was lower in the MPB‐infested stand despite higher basal area and plant area index of the other forests, suggesting that the desiccated needles serve as a less effective thermal buffer against longwave radiative losses. Eddy covariance observations of sensible and latent heat flux indicate that they are of similar but opposite magnitude, on the order of 20 MJ m^?2 during the melt period. Further analyses reveal that net turbulent energy fluxes were near zero because of the temperature and atmospheric vapour pressure encountered during the melt period. Future research should place snow science in the context of forest succession and management and address important uncertainties regarding the timing and magnitude of needlefall events. Copyright © 2015 John Wiley & Sons, Ltd.     

New insights into precious metal enrichment on the Isle of Rum,Scotland

The Rum Layered Suite (NW Scotland) is generally regarded as one of a handful of classic examples of open‐system layered mafic‐ultramafic intrusions, or ‘fossilized’ basaltic magma chambers, world‐wide. The eastern portion of the Rum intrusion is constructed of sixteen repeated, coupled, peridotite–troctolite units. Each major cyclic unit has been linked to a major magma replenishment event, with repeated settling out of ‘crops’ of olivine and plagioclase crystals to form the cumulate rocks. However, there are variations in the lithological succession that complicate this oversimplified model, including the presence of chromitite (>60 vol. percent Cr‐spinel) seams. The ～2 mm thick chromitite seams host significant platinum‐group element (PGE) enrichment (e.g. ～2 ppm Pt) and likely formed in situ, i.e. at the crystal mush–magma interface. Given that the bulk of the world's exploited PGE come from a layered intrusion that bears remarkable structural and lithological similarities to Rum, the Bushveld Complex (South Africa), comparisons between these intrusions raise intriguing implications for precious metal mineralization in layered intrusions.     

Near‐surface hydraulic conductivity of northern hemisphere glaciers

The hydrology of near‐surface glacier ice remains a neglected aspect of glacier hydrology despite its role in modulating meltwater delivery to downstream environments. To elucidate the hydrological characteristics of this near‐surface glacial weathering crust, we describe the design and operation of a capacitance‐based piezometer that enables rapid, economical deployment across multiple sites and provides an accurate, high‐resolution record of near‐surface water‐level fluctuations. Piezometers were employed at 10 northern hemisphere glaciers, and through the application of standard bail–recharge techniques, we derive hydraulic conductivity (K) values from 0.003 to 3.519 m day^?1, with a mean of 0.185 ± 0.019 m day^?1. These results are comparable to those obtained in other discrete studies of glacier near‐surface ice, and for firn, and indicate that the weathering crust represents a hydrologically inefficient aquifer. Hydraulic conductivity correlated positively with water table height but negatively with altitude and cumulative short‐wave radiation since the last synoptic period of either negative air temperatures or turbulent energy flux dominance. The large range of K observed suggests complex interactions between meteorological influences and differences arising from variability in ice structure and crystallography. Our data demonstrate a greater complexity of near‐surface ice hydrology than hitherto appreciated and support the notion that the weathering crust can regulate the supraglacial discharge response to melt production. The conductivities reported here, coupled with typical supraglacial channel spacing, suggest that meltwater can be retained within the weathering crust for at least several days. Not only does this have implications for the accuracy of predictive meltwater run‐off models, but we also argue for biogeochemical processes and transfers that are strongly conditioned by water residence time and the efficacy of the cascade of sediments, impurities, microbes, and nutrients to downstream ecosystems. Because continued atmospheric warming will incur rising snowline elevations and glacier thinning, the supraglacial hydrological system may assume greater importance in many mountainous regions, and consequently, detailing weathering crust hydraulics represents a research priority because the flow path it represents remains poorly constrained.