应用免疫荧光技术对三种赤潮藻的研究

为探索和建立免疫学结合荧光技术对赤潮生物的定性、定量检测,制备了3种粒径不同的赤潮藻——赤潮异弯藻(Heterosigma akashiwo)、共生甲藻(Symbiodinium sp)、链状亚历山大藻(Alexandrium catenella)的多克隆抗体,并采用封闭吸附处理以提高抗体的特异性;采用间接酶联免疫法检测了抗体的效价;结果表明未经吸附处理的赤潮异弯藻、共生甲藻的效价分别为1:41 000,1:18 000以上,显示是高亲合力的抗体;链状亚历山大藻抗体的效价达到1:3 500,相比另两种藻的效价较低;吸附处理后的3种多克隆抗体的特异性明显提高,而效价有所下降。3种多克隆抗体与各自藻细胞均有很强的结合力,赤潮异弯藻和共生甲藻的荧光信号均匀分布于细胞表面,链状亚历山大藻细胞的荧光信号在细胞表面分布不均匀,主要集中在腹部横沟内。制备的多克隆抗体只与自身藻细胞结合,与另外2种藻细胞无交叉反应。链状亚历山大藻抗体只与自身藻细胞结合,与塔马亚历山大藻无交叉反应,可作为同属内不同种的鉴别。结合了免疫学和荧光技术,具有特异、灵敏、直观和简便的优点,并且费用低,对于开拓赤潮准确定性、定量检测方法和技术具有重要价值。     

Molecular identification of scallop planktonic larvae using species-specific microsatellites

The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However, the larvae identification by morphological characteristics is notoriously difficult, mainly due to the small size （usually being less than 150 μm） and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species, the Zhikong scallop Chlamys farreri, the noble scallop C. nobilis, the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers, which was specified by transferability analyses, assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1% -2% larvae in mixed plankton samples. Larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple, inexpensive and robust in identification analyses, and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years.     

验后平差方法在Geosat/GM卫星测高数据处理中的应用

为了消除多代卫星测高数据之间的不协调性,利用两步处理法对Geosat/GM正常点海面高数据进行自交叉点平差及与T/P卫星测高数据联合平差,发现误差模型的系数计算出现异常,通过分析误差模型及正常点海面高数据的分布特征,找出了出现异常的原因,对计算方法进行改进后,大大提高了计算结果的稳定性和可靠性。     

基于POM模式与blending同化法建立中国近海潮汐模型

利用POM海洋数值模式建立了中国近海（2°N-41°N,99°E～132°E）分辨率为5′×5′的潮汐模型,模式采用blending同化法同化了由10年TOPEX／Poseidon测高数据反演的潮汐参数与沿岸52个验潮站观测。精度分析表明建立的潮汐模型的8分潮RSS为12．5cm。     

Tidal Effects on the Bottom Thermal Front of North Yellow Sea Cold Water Mass near Zhangzi Island in Summer 2009

Three seabed-mounted TD/CTD chains and two upward-looking acoustic Doppler current profilers(ADCPs) in the southwest of Zhangzi Island are used and a simultaneous cruise observation in the northern North Yellow Sea(NYS) is conducted to study temperature variation in the bottom thermal front zone of the NYS Cold Water Mass(NYSCWM) during the summer of 2009. In the flood-ebb tidal cycles, the bottom temperature decreases(increases) during flood(ebb) tides, which are dominated by the tidal-current induced horizontal advection. The ebb tide-induced temperature increase is larger than the flood tide-induced temperature decrease due to seasonal warming. In the spring-neap tidal cycles, the temperature and the vertical temperature structure show notable fortnightly variation from 16 July to 25 August. The bottom temperature increases from neap to spring tides and decreases from spring to neap. The Richardson number demonstrates strengthened vertical mixing during spring tides but enhanced stratification during neap tides. The spring-neap variation in vertical shear caused by tidal current is the dominant factor that induces the fortnightly variation in vertical mixing and thus bottom temperature.     

基于水资源约束的中国城镇化SD模型与模拟

中国城镇化正处于快速发展阶段,尽管经济和社会发展主控要素还在发挥重要作用,但水资源在生产、生活和生态方面发挥主控作用的局面愈益明显。在中国城镇化系统动力学（system dynamics,SD）模型基础上,从水资源供给、需求和水环境等层面将水资源作为主控要素嵌入原有模型中,拓展出基于水资源约束的中国城镇化SD模型,并对水资源利用进行了多情景模拟。结果表明：① 系统存流量和灵敏度检验证明模型模拟效果良好,具有可操作性。② 部门用水效率一定时,产业发展对水资源供需平衡的影响比人口增长更为明显。③ 在实行节水农业、节水工业、高生活需水、高生态环境需水和高再生水利用的综合协调方案中,2050年中国城镇化的发展约共需6789.70亿 m³水资源,基本实现水资源供需平衡。     

Assessment of Desert Ecological Assets and Countermeasures for Ecological Compensation

Can deserts be transformed into resources, into assets, and further into cash? It is necessary to scientifically assess desert ecological assets and incorporate them into the national economic accounting system and the current evaluation system for socio-economic development. This study will provide a scientific basis and robust data for establishing a target system that is compatible with both ecological civilization and an associated reward and punishment mechanism, as well as for designing and implementing effective compensation policies for desert ecosystems. This paper first defines desert ecological assets, and then develops a framework for assessing them based on the evaluation of desert ecological resources and desert ecosystem services. This framework paves the foundation for quantitatively assessing desert ecological assets and preparing balance sheets of desert ecological assets. Finally, this paper analyzes current policies relating to desert ecological compensation, discusses how to design compensation policies based on assessment of desert ecological assets, and puts forward suggestions for improving current policies.     

海底爆破振动强度衰减规律及中华白海豚保护措施研究

为确保海底爆破施工所诱发的振动不对中华白海豚造成危害,基于量纲分析法对萨道夫斯基经验公式进行修正,建立适用于预测海底爆破质点峰值振动速度的相似准数方程。在此基础上根据现场试验数据进行非线性回归运算,得到相关参数并分析了海底爆破振动强度衰减规律。分析表明:海底爆破质点峰值振速主要由单段装药量、监测点距爆心水平距离、覆盖层厚度及海水深度等因素决定。将参数值分别代入萨氏公式及新建公式计算预测值并进行误差估计,其平均误差分别为20.3%和10.0%,新建公式提高了对质点振速预测的准确性。利用新建公式及超压内安全振速估算保护中华白海豚的安全距离并提出相应的保护措施,为同类工程施工提供理论参考依据。     

基于国产高分遥感的人工种植红树林种间分类方法研究——以广西茅尾海红树林为例

红树林的种间结构组成对红树林生态系统的健康和发展至关重要,而红树林种间分类问题一直以来都是基于遥感手段的红树林监测中的难点。针对该问题,以人工种植为特点的广西茅尾海红树林遥感种间分类为例,基于面向对象的分类思想,提出了一种现场样本与分割对象相结合的红树林种间分类方法。利用GF-2 PMS1高分辨率卫星遥感影像数据,开展了广西茅尾海红树林湿地典型植被精细分类和空间分布研究,并将分类结果与基于像素和传统面向对象SVM分类方法进行了对比。结果显示:总体上,面向对象分类方法更适合用于茅尾海红树林湿地典型植被分类;对于局部混生明显的区域使用基于像素SVM分类方法效果会更好;传统面向对象分类方法中将整个影像分割对象单元作为训练样本可能会在某种程度上造成负面影响。因此,使用文中提出的样本选择新方法进行面向对象分类精度最高,总体精度达到了93.13%,Kappa为0.89。     

声速剖面EOF表示的第一模态解析

为实现对声速剖面EOF表示后第一模态时间系数和空间函数变化规律的解析,提出了一种简化的声速剖面变化模型,即"拐点"深度值和声速值的变化;声速梯度的变化和表层海水温度周期性变化所引起的海水声速变化,通过将4种因素所引起的第一模态空间函数的变化规律与实际声速剖面簇第一模态空间函数的变化规律对比,分析引起实测声速剖面变化的主要因素,最后,分别用深海和浅海实测声速剖面数据对其进行验证。