Characteristics of photosynthetic pigment composition ofGymnodinium mikimotoi Miyake etKominami exOda

Gymnodinium mikimotoi, a senior synonym ofGymnodinium nagasakiense often causes red tides in coastal waters of the western part of Japan. The photosynthetic pigment composition of two strains ofG. mikimotoi were analyzed by HPLC. They contain chlorophyllc ₃ which has not been reported from dinoflagellates. They also contain fucoxanthin, 19'-hexanoyloxyfucoxanthin and 19'-butanoyloxyfucoxanthin as major carotenoides, which are anomalous in dinoflagellates. The pigment composition ofG. mikimotoi is compared with that ofGyrodinium aureolum which occur in European waters and is thought as a conspecific species withG. mikimotoi by several authors.     

Mixed layer depth front and subduction of low potential vorticity water under seasonal forcings in an idealized OGCM

The mixed layer depth (MLD) front and subduction under seasonal variability are investigated using an idealized ocean general circulation model (OGCM) with simple seasonal forcings. A sharp MLD front develops and subduction occurs at the front from late winter to early spring. The position of the MLD front agrees with the curve where \({\rm D}T_{\rm s}/{\rm D}t = \partial T_{\rm s} /\partial t + {\user2{u}}_{\rm g} \cdot \nabla T_{\rm s} = 0\) is satisfied (t is time, \({\user2{u}}_{\rm g}\) is the upper-ocean geostrophic velocity, \(T_{\rm s}\) is the sea surface temperature (SST), and \(\nabla\) is the horizontal gradient operator), indicating that thick mixed-layer water is subducted there parallel to the SST contour. This is a generalization of the past result that the MLD front coincides with the curve \({\user2{u}}_{\rm g} \cdot \nabla T_{\rm s} = 0\) when the forcing is steady. Irreversible subduction at the MLD front is limited to about 1 month, where the beginning of the irreversible subduction period agrees with the first coincidence of the MLD front and \({\rm D}T_{\rm s}/{\rm D}t =0\) in late winter, and the end of the period roughly corresponds to the disappearance of the MLD front in early spring. Subduction volume at the MLD front during this period is similar to that during 1 year in the steady-forcing model. Since the cooling of the deep mixed-layer water occurs only in winter and SST can not fully catch up with the seasonally varying reference temperature of restoring, the cooling rate of SST is reduced and the zonal gradient of the SST in the northwestern subtropical gyre is a little altered in the seasonal-forcing case. These effects result in slightly lower densities of subducted water and the eastward shift of the MLD front.     

Jellyfish patch formation investigated by aerial photography and drifter experiment

Jellyfish patch formation is investigated by conducting a drifter experiment combined with aerial photography of a sustained patch of the moon jellyfish in Hokezu Bay, Japan. Jellyfish patches are aggregations of individuals that are caused by a combination of swimming (active influence) and advection by currents (passive influence). The drifter experiment involved the injection of 49 drifters around a distinct surface patch of jellyfish within an area of approximately 300 m × 300 m. The drifters’ motion, caused only by the passive influence, was recorded in a series of 38 aerial photographs taken over approximately 1 h. The ambient uniform current field larger than the patch scale was estimated from the movement of the centroid position of drifters, while the distribution of horizontal divergence and relative vorticity around the patch was estimated from the time-derivative in areas of triangles formed by the drifters. The centroid positions of both drifters and patches moved stably toward the bay head at different speeds. The difference vector between the patch and drifter centroids was directed to the sun, and was opposite to the ambient current. The distributions of vorticity and divergence around patches exhibited inhomogeneity within the patch scale, and the drifters in this nonuniform current field aggregated near the convergence area within 1 h. The results suggest that horizontal patch formation is predominantly influenced by passive factors at the surface of Hokezu Bay. Furthermore, the upward swimming against downwelling may make sustained patch in surface layer.     

Comparison of the acid-base responses to CO2 and acidification in Japanese flounder (Paralichthys olivaceus)

To investigate whether the biological toxicity of aquatic hypercapnia is due to the direct effects of CO2 or to the effects of acidification of seawater by CO2, the Japanese flounder (Paralichthys olivaceus) was subjected to seawater equilibrated with a gas mixture of air containing 5% CO2 (pH 6.18) or seawater acidified to the same pH with 1 N H2SO4. All the fish died within 72 h in the CO2 exposure group, whereas no mortality occurred in the acid group. Acid-base parameters as well as plasma ion concentrations were severely perturbed in the CO2 exposure group, whereas they were minimally affected in the acid group. These results clearly demonstrate that the mortality in the CO2 group is a direct result of the elevated levels of dissolved CO2 and not to the effects of the reduced water pH.     

Growth stimulation and inhibition of natural phytoplankton communities by model organic ligands in the western subarctic Pacific

The influence of organic ligands on natural phytoplankton growth was investigated in high-nitrate low-chlorophyll (HNLC) waters and during a phytoplankton bloom induced by a mesoscale iron enrichment experiment (SEEDS II) in the western subarctic Pacific. The growth responses of the phytoplankton in the treatments with iron complexed with model ligand were compared with those with inorganic iron or a control. Desferrioxamine B and protoporphyrin IX were used as models for hydroxamate-type siderophore and tetrapyrrole-type cell breakdown ligand, respectively. In the HNLC water, iron associated with protoporphyrin IX especially stimulated smaller phytoplankton (<10 μm) growth, 1.5-fold more than did inorganic iron. Surprisingly, only the addition of protoporphyrin IX stimulated small phytoplankton growth, suggesting that these cell breakdown ligands might be more bioavailable for them. The protoporphyrin IX’s stimulatory effect on small phytoplankton was not observed during bloom decline phase. The growth of phytoplankton was inhibited in the treatment with desferrioxamine B-complexed iron, suggesting its low bioavailability for the natural phytoplankton community. Its inhibitory effects were particularly pronounced in pico-eukaryotic phytoplankton. During the iron-induced bloom, the phytoplankton’s iron-stress response gradually increased with the desferrioxamine B concentration, suggesting that the competition for iron complexation between natural ligands and desferrioxamine B affected phytoplankton growth. However, the pico-eukaryotes did seem better able to utilize the desferrioxamine B-complexed iron during the bloom-developing phase. These results indicate that the iron bioavailability for phytoplankton differs between bloom-developing and bloom-decline phases.     

Satellite detection of red tide in Ariake Sound, 1998–2001

High resolution SeaWiFS data was used to detect red tide events that occurred in the Ariake Sound, Japan, a small embayment known as one of the most productive areas in Japan. SeaWiFS chlorophyll data clearly showed that a large red tide event, which damaged seaweed (Nori) cultures, started early in December 2000 in Isahaya Bay, expanded to the whole sound and persisted to the end of February 2001. The monthly average of SeaWiFS data from May 1998 to December 2001 indicated that the chlorophyll peaks appeared twice a year, in early summer and in fall, after the peaks of rain and river discharge. The SeaWiFS data showed that the red tide event during 2000–2001 winter was part of the fall bloom; however, it started later and continued significantly longer than other years. Satellite ocean color data is useful to detect the red tide; however the algorithms require improvement to accurately estimate chlorophyll in highly turbid water and in red tide areas.     

Solar Wind Speed and Expansion Rate of the Coronal Magnetic Field in Solar Maximum and Minimum Phases

Relationships between solar wind speed and expansion rate of the coronal magnetic field have been studied mainly by in-ecliptic observations of artificial satellites and some off-ecliptic data by Ulysses. In this paper, we use the solar wind speed estimated by interplanetary scintillation (IPS) observations in the whole heliosphere. Two synoptic maps of SWS estimated by IPS observations are constructed for two Carrington rotations CR 1830 and 1901; CR 1830 starting on the 11th of June, 1990 is in the maximum phase of solar activity cycle and CR 1901 starting on the 29th of September, 1995 is in the minimum phase. Each of the maps consist of 64800 (360×180) data points. Similar synoptic maps of expansion rate of the coronal magnetic field (RBR) calculated by the so-called potential model are also constructed under a radial field assumption for CR 1830 and CR1901. Highly significant correlation (r=–0.66) is found between the SWS and the RBR during CR1901 in the solar minimum phase; that is, high-speed winds emanate from photospheric areas corresponding to low expansion rate of the coronal magnetic field and low speed winds emanate from photospheric areas of high expansion rate. A similar result is found during CR 1830 in solar maximum phase, though the correlation is relatively low (r=–0.29). The correlation is improved when both the data during CR 1830 and CR 1901 are used together; the correlation coefficient becomes –0.67 in this case. These results suggest that the correlation analysis between the SWS and the RBR can be applied to estimate the solar wind speed from the expansion rate of the coronal magnetic field, though the correlation between them may depend on the solar activity cycle. We need further study of correlation analysis for the entire solar cycle to get an accurate empirical equation for the estimation of solar wind speed. If the solar wind speed is estimated successfully by an empirical equation, it can be used as an initial condition of a solar wind model for space weather forecasts.     

The Tokyo PMC catalog 85

The catalog of positions of 1007 stars (792 FK4 and FK4S stars, 57 OB stars, 49 NPZT stars, and 109 SAO stars) is presented. They were observed during the period from December 1984 to September 1985 with the Tokyo Photoelectric Meridian Circle (Tokyo PMC). The positions in the catalog are referred to the equinox and equator of J2000, and are based on the FK4 system. The internal errors of a single observation were estimated to be ( cos, )=(0.16, 0.19), whereas the mean internal errors of the catalog positions were (0.08, 0.08) for FK4 stars and (0.09, 0.11) for FK4S stars. A comparison of the positions of the FK4 stars in the present catalog with those of the FK4 catalog shows significant differences cos and in some declination zones. Some of those differences are commonly found in other recent catalogs. Thus they may be considered to be real systematic errors in the FK4 system. Neither significant magnitude nor color equations exist in the Tokyo PMC 85 catalog.     

A shipborne wave-recording system with digital data processing

A shipborne wave-recording system which consists of a sonic wave gauge, accelerometers, gyroscopes and a computer system is described. Signals from the measuring apparatus are fed directly into a shipborne digital computer system at a prescribed sampling rate. The time series of wave heights and the acceleration are transformed into Fourier series using an algorithm of Fast Fourier Transform. Errors contained in the observed wave heights due to ship motion are corrected in the Fourier series by using the Fourier coefficients for the vertical acceleration. Power spectra and waveforms can also be calculated in a short time with this system from Fourier coefficients. Examples of the observational results obtained in the central part of the East China Sea in 1969 are presented.     

A new fluorometric method of analysis of selenium in sea water

A new method of determination of selenium and separation of Se(IV) and Se(VI) in sea water is described. The selenium is determined by fluorometric method using Se-DAN complex in cyclohexane media. Prior to the fluorometric determination, Se(IV) is separated from sea water by means of Se(IV)-DDTC complex which is adsorbed on the macroreticular resin. As to the separation of the total selenium from sea water sample, the reduction and coprecipitation method is used. Se(VI) is determined with the same method as used for the total selenium after the separation of Se(IV). The average recoveries are 92.5±1.3% for Se(IV) and 97.4±0.9% for Se. The standard deviation of analytical results is below 10%.