Simultaneous quantification of dissolved organic carbon fractions and copper complexation using solid-phase extraction

Trace metal cycling in natural waters is highly influenced by the amount and type of dissolved organic C (DOC). Although determining individual species of DOC is unrealistic, there has been success in classifying DOC by determining operationally defined fractions. However, current fractionation schemes do not allow for the simultaneous quantification of associated trace metals. Using operational classifications, a scheme was developed to fractionate DOC based on a set of seven solid-phase extraction (SPE) cartridges. The cartridges isolated fractions based on a range of specific mechanisms thought to be responsible for DOC aggregation in solution, as well as molecular weight. The method was evaluated to determine if it can identify differences in DOC characteristics, including differences in Cu–DOC complexation. Results are that: (1) cartridge blanks were low for both DOC and Cu, (2) differences are observed in the distribution of DOC amongst the fractions from various sources that are consistent with what is known about the DOC materials and the mechanisms operative for each cartridge, (3) when present as a free cation, Cu was not retained by non-cationic cartridges allowing the method to be used to assess Cu binding, (4) the capability of the method to provide quantitative assessment of Cu–DOC complexation was demonstrated for a variety of DOC standards, (5) Cu was found to preferentially bind with high molecular weight fractions of DOC, and (6) estimated partitioning coefficients and conditional binding constants for Cu were similar to those reported elsewhere. The method developed describes DOC characteristics based on specific bonding mechanisms (hydrogen, donor–acceptor, London dispersion, and ionic bonding) while simultaneously quantifying Cu–DOC complexation. The method provides researchers a means of describing not only the extent of DOC complexation but also how that complex will be behave in natural waters.     

新疆希勒库都克铜钼矿区岩浆混合作用: 来自锆石U-Pb年代学的证据

新疆北部富蕴县内希勒库都克铜钼矿区的花岗闪长岩及其包体岩相学、矿物化学和岩石地球化学特征及野外地质特征显示其为岩浆混合作用的结果。本文获得花岗闪长岩及其中暗色微粒包体玄武安山玢岩、细粒辉长闪长岩锆石U-Pb年龄为326.8±2.1Ma、327.6±2.4Ma、329.3±2.3Ma,年龄值基本一致,这一结果从年代学角度为花岗闪长岩及其中暗色微粒包体的岩浆混合作用成因提供证据。偏酸性的花岗闪长质与偏基性玄武安山质岩浆混合作用形成了331.9±2.1Ma的安山玢岩脉。     

滇西三江地区中新生代“盆山”耦合运动学过程

滇西三江地区中新生代盆山格局是研究盆山耦合的典型实例。本文通过解析该区叠加构造变形期次和样式,理清其中新生代的盆山耦合关系,并探讨思茅盆地对相邻造山演化的沉积响应过程。研究表明,三江地区经历了主碰撞造山带与前陆盆地耦合(T1-T2)、后碰撞造山带与伸展盆地耦合(T3-K)以及陆内"盆山"共变(E-Q)三个盆山耦合运动学过程。兰坪-盆地内复理石、山前磨拉石到红色碎屑岩的沉积序列响应了相邻造山带从主碰撞阶段向后碰撞阶段的演化,该阶段盆地转换过程受控于拆沉作用和岩浆底侵作用等深部动力学机制。     

一株分离自太平洋卡罗琳深海海山水域NX-5T菌株的多相分类鉴定

黄杆菌种类多、分布广,但从深海海山环境中发现黄杆菌新物种的报道还没有。2017年我们从西太平洋卡罗琳深海海山水域分离到一株细菌XN-5^T,经16S rRNA基因序列测定表明,该菌与黄杆菌属(Flavobacterium)内系统发育关系最近模式菌株F.degerlachei LMG 21915^T、F.frigoris LMG 21922^T之间的相似性分别为97.5%和97.8%。本研究采用形态学观察、生理生化特征、遗传特征、脂肪酸和极性脂测定等方法,对该菌株进行了详细的多相分类鉴定。结果表明,XN-5^T菌株在16S rRNA基因系统发育、生长温度、淀粉、酪蛋白水解、硝酸盐还原,产α-半乳糖苷酶、α-葡萄糖苷酶、β-葡萄糖苷酶,同化L-阿拉伯糖、D-甘露醇、N-乙酰-氨基葡萄糖、葡萄糖酸钾,发酵蔗糖、阿拉伯糖,以及脂肪酸、极性脂组成等方面,均与相近模式菌株有所差异。综合多相分类鉴定数据,我们判定XN-5^T菌株为Flavobacterium属内的一个新种,命名为Flavobacterium pacifica sp. nov。     

南极菲尔德斯半岛潮间带沉积物细菌的多样性及分离菌产酶测定

摘要:南极菲尔德斯半岛具有多种不同特征的生态地理微环境,如长城科考站、企鹅岛、生物湾与黄金湾流域、半岛南部、碧玉滩等。这些区域因水文地质、动植物分布、人类活动程度等不同而具有明显的生态地理差异。本研究从这些微环境海岸潮间带采集了7份代表性沉积物样品,采用16S rRNA基因高通量测序方法,对其细菌类群的多样性以及环境理化因子的影响进行了比较分析;同时采用常规可培养鉴定方法,对样品的分离菌株产酶状况进行了初步测定。结果表明：整体上看,所有样品的细菌类群主要分布在45门、104纲、442属当中,表现出比较高的多样性。其中,优势菌门Proteobacteria主要分布在半岛南部、碧玉滩和生物湾潮间带沉积物物中,优势菌门Bacteroidetes主要分布在企鹅岛两侧和黄金湾潮间带沉积物,长城站潮间带沉积物的优势门为Firmicutes。相似生态微环境的潮间带沉积物具有不同的优势菌门和纲;而不同生态微环境潮间带沉积物却具相同的优势菌门和纲。有机氮（TON）、NH4+-N、NO3--N、PO43--P、NO2--N含量与企鹅岛两侧、生物湾、黄金湾潮间带沉积物的菌群多样性具有相关性,其中TON对生物湾样品的影响最大,而有机碳（TOC）对所有样品的影响较小。分离菌株的产酶实验表明：在企鹅岛、生物湾、黄金湾等动物频繁出没的潮间带样品中,蕴藏着一批产淀粉酶、酪蛋白酶、脂肪酶、七叶苷酶、过氧化氢酶能力较强菌株,这为今后进一步的应用开发提供了宝贵的低温产酶菌株来源。 关键词: 菲尔德斯半岛; 潮间带沉积物; 细菌类群多样性; 产酶菌株     

Historical Sediment Record of ~(137)Cs, δ-HCH,and δ~(13)C Reflects the Impact of Land Use on Soil Erosion

This paper reports the concentrations of 137 Cs, hexachlorocyclohexane(HCH), dichlorodiphenyltrichloroethanes(DDT) and its main degradation products, δ13C, and organic carbon in pond sediments(0-210 cm, sectioned by 2-20 cm interval) and surface soils(the 0-3 cm horizon) collected in 2010 from Chenjia catchment, which is located in Yanting county in the hilly central Sichuan of China. α-, δ-, and γ-HCH, DDT, and DDD were not detected throughout the sediment profile. Trace concentrations of δ-HCH(0.89-29.31 ng g-1) and p,p′-DDE(1.85-6.02 ng g-1) were detected only in top 40 cm sediment. The 137 Cs fallout peak in 1963(corresponding to the 55-60 cm depth), the sedimentary signature left by the last year of HCH use in 1989(an additional indicator at 20-25 cm), and the obvious original channel bed prior to the construction of the pond in 1956 were used as temporal markers to estimate changes in average sedimentation rate between different periods due to changes in land use. Continuous, marked decrease in average sedimentation rate(i.e., 3.79, 1.35 and 1.07 cm year-1 in 1956-1963, 1963-1989, and 1989-2010, respectively)over time was observed, probably due to the reforestation, abandoning of steep sloping farmland for afforestation and natural re-vegetation(implementation of the Grain for Green Program), and the conversion of part of gently sloping farmland terraces to orchard land since the 1980 s, especially since the 1990 s. This was corroborated by the observed decrease(more negative) in δ13C of sediment towards the surface, which indicates increased relative contribution of eroded soil particles coming from slopes with increased tree cover in sediment source area. Combined use of 137 Cs, δ-HCH, and δ13C record in sediments has been demonstrated to be a powerful approach to reconstruction of response in sedimentation rate to historical land use changes.     

渤海海峡亚海鲂属鱼类一新记录种——云纹亚海鲂

2012年12月3日,在渤海海峡的蓬莱近海浅水海域捕获雄性成体鱼1尾,该鱼体长34 cm,体高18cm,体长为体高的1.9倍,体宽4.5 cm,头长12.5 cm,体长为头长的2.7倍,尾柄长4.5 cm,体光滑无鳞。管状侧线前半部弧形,后半部平直,行于体中央。棘状骨板沿背鳍基底两侧各12块,臀鳍基底两侧各8块,每一骨板中央具1棘,向后;胸部6块,自第3骨板开始分左右两行;腹部7块,自第2骨板开始分左右两行,胸腹部骨板棘小,形成锯齿缘。背鳍1个,鳍棘部与鳍条部之间有一深缺刻;鳍棘细长,棘间鳍膜延长成丝状,最长超过尾鳍末端。据其形态特征和测量值,鉴定为云纹亚海鲂(Zenopsis nebulosa Temminck et Schlegel,1847),为该海域首次记录。     

莺歌海盆地前新生代基底特征

结合盆缘露头、盆地基底钻井及重磁震资料,综合研究莺歌海盆地前新生代基底特征。基于盆地西北缘Song Da带18个剖面点地层序列、166件岩石样品密度和2800套磁化率测量结果,建立密度--磁化率交汇图版,约束盆内地震剖面和重磁异常解释,通过海陆结合的方法填制出盆地基底地质图。其前新生界由前震旦系、寒武系-上三叠统下部、上三叠统上部-白垩系三个构造层构成,它们沿红河断裂呈北西向分布,中间老,为前震旦纪中-高级变质岩;两侧新,向西为中生代沉积岩,向东为古生代浅变质岩、灰岩。     

The Value of Practice-Based Knowledge

Increasing recognition of the value of practice-based or experiential knowledge in natural resource management justifies the creation of a new category of articles in Society & Natural Resources that we are calling Practice-Based Knowledge (PBK). The rationale for focusing on PBK is due to its key role in the emergence of hybrid governance institutions across state, market, and civil society, understanding the complexity of dynamic socioecological systems, recognizing the challenges of multiple knowledge systems and context-specific practices, embracing the power of informal institutions and civic science, and engaging debates on the growing prevalence of market-oriented conservation. The goal is to provide a dedicated space within the published, peer-reviewed literature for scholars, government officials, nonprofit managers, and engaged citizens to share experiences informed by practical action. Relevant and timely practice-based insights may improve understanding and management of social and ecological processes and systems, while also offering the potential to contribute to theory.     

农业技术扩散中的农户采用行为研究:国外进展与国内趋势

农户是我国农区的微观经济单元,是农业技术采用的行为主体。以农户为对象的农业技术扩散采用行为的研究对于诱导农户行为、提高农业技术的采用效率、促进农区发展具有重要的理论和应用价值。从研究视角、研究方法、研究内容3个方面对国内外相关研究成果进行较为系统的梳理,总结评述最新进展和特点,并在此基础上对农户技术采用行为的研究趋势进行展望,希冀有助于推进国内对农户技术采用行为的深入研究,进而为促进农业技术扩散与推广、提高农业科技成果转化率提供参考。