Cloning and phylogenetic analysis of a fatty acid elongase gene from Nannochloropsis oculata CS179

Nannochloropsis oculata CS179, a unicellular marine microalga, is rich in long-chain polyunsaturated fatty acids (LCPUFAs). Elongase and desaturase play a key role in the biosynthesis of PUFAs. A new elongase gene, which encodes 322 amino acids, was identified via RT-PCR and 5′ and 3′ RACE. The sequence of the elongase gene was blast-searched in the NCBI GenBank and showed a similarity to those of the cryptosporidium. But the NJ-tree revealed that the N. oculata CS179 elongase clustered with those of the microalgae Phaeodactylum tricornutum, Ostreococcus tauri and Thalassiosira pseudonana.     

坚持土地节约集约利用促进文登经济和谐发展

文登市位于山东半岛东部,总面积1 779.84km2。辖14个镇、3个街道办事处、2处省级开发区,810个行政村、88个居委会,总人口64.25万人,其中农村人口46.2万人。全市耕地面积62 137.93hm2,人均耕地不足0.084 hm2,低于全国和全省的平均水平,土地供需矛盾日益突出。     

The study on a new technology of waterway information system

It is a lot limitations to survey and design waterway by the CAD technique,due to timely variation of hydrology and ecology in the complicated estuary area,such as Changjing River estuary.In this paper,the advantages of GIS technique is applied to manage and process the multi kind,multi scale and multi time hydrographic parameters,including water depth,current velocity,tidal,waves,wind speed,temperature and salinity etc.,also to deal with different projection,different scale charts.Meanwhile,in the paper,combination techniques of GIS and CAD has been developed for unification of the charts for efficient management,two-way automatic carts conversion between GIS and CAD,automatic cutting and assemble of a number of Charts,flight path of the projection of spatial data and coordinate transformation.This new technique has been applied to survey and design waterway of Changing River estuary.The result shows that the combination technology of GIS and CAD has application potentiality in the waterway survey and design.     

海洋毒素大田软海绵酸完全抗原的制备与分析

为了建立大田软海绵酸(Okadaic acid,0A)的免疫学检测方法,利用活泼酯法将OA分子中的羧基与栽体蛋白上的氨基偶联,人工制备OA的免疫抗原(OA-IgG)和检测抗原(OA-BSA),经电泳、动物实验及ELISA法进行了鉴定,完全抗原的偶联成功,为利用杂交瘤技术制备OA的单克隆抗体和海产品OA免疫学检测方法奠定了良好的基础.     

Elastic-plastic analysis for pile-anchor supporting system of deep foundation pit

By using numerical analysis methods to simulate the deep excavation, a lot of analyses are established on the basis of two-dimensional plane strain, ignoring the fact that foundation pit possesses three dimensions. For soil constitutive relation, people always take linear and nonlinear model, without considering the plastic behavior of soil. Using plastic-elastic hardening model to simulate constitutive relation of soil characteristics, the authors carried out mechanical analysis for pit excavation and support. The results show that the analysis for the stress state of pile anchor system is an effective way which provides theoretical basis for calculation of soil displacement.     

基于FLUENT的海洋内孤立波数值水槽模拟

基于FLUENT商业软件及其二次开发功能,在标准κ-ε湍流模型下,采用VOF方法追踪两层流体内界面,通过给定入口速度和水位的设置造波边界法,建立了可有效模拟弱非线性内孤立渡的分层流数值水槽,并与仿物理的双板造波方法进行了比较.采用设置边界法所造的波形与理论值符合较好,这为数值分析内孤立渡与海洋结构物相互作用问题提供了一条更加有效的途径.     

大连港外海域船舶尾迹光学特征研究

通过大连港外海上实验测量船舶含气泡尾迹表观光学特征(如离水辐亮度、遥感反射率),分析了尾迹气泡对极度混浊的Ⅱ类水体海水表观光学特征的影响.实验结果证明:无论在可见光还是近红外波段,尾迹气泡都增强了海水表观光学量,如"耘海"船尾迹遥感反射率在可见光波段相对背景海水提高了50%,在近红外波段相对提高了159%.数据分析结果表明在此种水体中,气泡使得尾迹海水水色变黄,而不是在Ⅰ类水体中使水色变绿.随着观测点在尾迹中的位置靠近目标船,气泡数密度不断增加,遥感反射率也随之增强.     

绿洲城市化与生态环境互动作用关系研究进展

绿洲城市化与生态环境互动作用关系研究经历了萌芽、发展和多元化研究三个阶段。近年 来, 国内外学者继承了绿洲城市化与自然环境变迁关系的历史学分析, 多角度探讨了绿洲古城国 产生、繁荣和衰落过程中的自然因素和人为因素作用; 十分重视水资源约束下的流域绿洲城市化 研究, 基本确立水资源约束和上、中、下游协调发展为研究切入点; 非常注重绿洲城市化与生态环 境相互作用机理探究, 从中、长时间尺度分析了绿洲城市化与生态环境的耦合关系及其阶段性特 征; 广泛采用空间技术、复杂系统分析和数学分析等定量方法评估了城市化与生态环境的响应过 程; 普遍关注绿洲城市化对生态环境正负效应的辨析; 逐步加强对可持续绿洲城市化模式和对策 的探索, 并总结了绿洲城市化与生态环境协调发展的经验与教训。从研究趋势来看, 今后在研究 的可控性与对实践的指导性、多学科交叉研究和系统动态性研究等方面将得到加强。     

基于J2EE和ArcIMS的地质灾害危险性评估项目备案系统

本文对J2EE和ArcIMS的地质灾害危险性评估项目备案系统的总体框架结构、系统三层体系结构、模块化思想设计要点和实现的技术路线及关键技术。系统应用Struts技术、数据库技术、ArcIMS的WebGIS技术和网络技术,建立了通用地理信息功能与地质灾害危险性评估项目备案自动审批的分布式软件系统,实现了地质灾害危险性评估项目备案的自动审批、管理、分析、决策、发布的一体化平台。该系统为有关部门提供辅助决策支持和信息发布服务。