极限饱和卤水

<正> 关于天然水中盐类的极限浓度和可能的溶解度问题研究得非常不足。虽曾预料在地壳内部将发现浓度很大的水层,可是直到若干年前还根本不知道有比重大于1.2—1.3的地下卤水。最近在东西伯利亚和中亚发现的地下卤水以及早些时候只在美国发现的地下卤水提供了这方面的资料。这种卤水的矿化度高、氯化钙含量占绝对优势,比重为     

应用模式识别法选拔学术带头人

讨论了与学术带头人培养、选拔有关的几个主要问题,并探讨了利用模式识别方法选拔学术带头人的可能性。     

基于局部重力场建模的Tikhonov正则化点质量核径向基函数方法

针对点质量核径向基函数应用于局部重力场建模中的设计矩阵严重病态问题,本文引入Tikhonov正则化方法对传统点质量核径向基函数方程进行改造,建立了相应的正则化模型。通过模拟数据进行仿真实验,以传统格网化方法作为对比试验,利用"标靶法"确定两种模型的最优结构。实验结果表明:正则化点质量核径向基函数可以直接利用离散数据进行局部重力场建模。在两种模型的最优结构下,当实测数据无污染时,正则化方法达到与传统格网化方法相当的精度;当实测值中加入3 mGal的高斯白噪声时,正则化方法的精度获得了27.9%的提升。这说明本文方法可以应用于局部重力场建模中,且模型结构更优,抗干扰能力更强。     

FG5-240绝对重力观测结果分析

介绍FG5-240对中国大陆及海岛17个测站进行绝对重力观测的情况,并对观测结果进行分析。结果表明FG5-240具有良好的稳定性,观测精度优于±5.00×10-8ms-2,满足绝对重力测定技术要求。     

广域多项式在扰动引力场逼近中的应用

利用广域多项式对某块区域扰动引力进行逼近计算时,需要用到与主域单元相接的24个次域单元节点值,当单元位于研究区域顶点等特殊位置时,不能利用所有次域单元节点,因而出现逼近精度降低的现象。针对此情况,比较了广域多项式与反距离加权两种方法的精度与效率,并提出改进,改善逼近效果。     

Molecular cloning and mRNA expression analysis of myosin heavy chain (MyHC) from fast skeletal muscle of grass carp, Ctenopharyngodon idella

The myosin heavy chain(MyHC)is one of the major structural and contracting proteins of muscle.We have isolated the cDNA clone encoding MyHC of the grass carp,Ctenopharyngodon idella. The sequence comprises 5 934 bp,including a 5 814 bp open reading frame encoding an amino acid sequence of 1 937 residues.The deduced amino acid sequence showed 69%homology to rabbit fast skeletal MyHC and 73%–76%homology to the MyHCs from the mandarin fish,walleye pollack,white croaker,chum salmon,and carp.The putative sequences of subfragment-1 and the light meromyosin region showed 61.4%–80%homology to the corresponding regions of other fish MyHCs.The tissue-specific and developmental stage-specific expressions of the MyHC gene were analyzed by quantitative real-time PCR.The MyHC gene showed the highest expression in the muscles compared with the kidney,spleen and intestine.Developmentally,there was a gradual increase in MyHC mRNA expression from the neural formation stage to the tail bud stage.The highest expression was detected in hatching larva.Our work on the MyHC gene from the grass carp has provided useful information for fish molecular biology and fish genomics.     

基于重力垂直梯度的欧拉反褶积改进措施

欧拉反褶积反演解的发散与不稳定性一直制约着其实用效果。为此,提出利用垂直梯度反演和筛选准则并在分辨信号极值区域基础上进行重力反演等改进方法。通过模型试算,全面分析它们相对常规重力数据反演方法的效果。结果表明,改进方法能明显提高反演解水平位置和深度的精度和收敛性。     

陕南鲁家坪组小壳动物化石的发现及其意义

小壳动物化石产于川、陕、鄂三省边界地区的鲁家坪组中(图1)。该组岩性为一套轻变质的浅海相沉积建造,厚700—900米,地层层序较清楚,分层标志明显。其下部为炭质板岩、含钙泥板岩及白云岩,中部为硅质岩及硅质板岩,上部为炭质板岩夹泥灰岩。该组地层在下寒武统含俞氏库蜒虫的箭竹坝组之下,超覆于下震旦统耀岭河群火山岩之上。原建组者将其统划为下寒武统((?)_1L)。七十年代以来,多数研究者认为该组下部有上震旦统存在,但未找到化石作为依据,分层界线有较大争议。一种意见将该组中部硅质岩(相当本文(?)_1L~2层)及其以     

深海嗜热菌Geobacillus sp. EPT3锰超氧化物歧化酶的纯化与生物化学鉴定

Thermostable SOD is a promising enzyme in biotechnological applications. In the present study, thermophile Geobacillus sp. EPT3 was isolated from a deep-sea hydrothermal field in the East Pacific. A thermostable superoxide dismutase(SOD) from this strain was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography, and Phenyl-Sepharose chromatography. SOD was purified 13.4 fold to homogeneity with a specific activity of 3 354 U/mg and 11.1% recovery. SOD from Geobacillus sp. EPT3 was of the Mn-SOD type, judged by the insensitivity of the enzyme to both KCN and H2O2. SOD was determined to be a homodimer with monomeric molecular mass of 26.0 k Da. It had high thermostability at 50°C and 60°C. At tested conditions, SOD was relatively stable in the presence of some inhibitors and denaturants, such as β-mercaptoethanol(β-ME), dithiothreitol(DTT), phenylmethylsulfonyl fluoride(PMSF), urea, and guanidine hydrochloride. Geobacillus sp. EPT3 SOD showed striking stability across a wide p H range from 5.0 to 11.0. It could withstand denaturants of extremely acidic and alkaline conditions, which makes it useful in the industrial applications.