深海嗜热菌Geobacillus sp. EPT3锰超氧化物歧化酶的纯化与生物化学鉴定

Thermostable SOD is a promising enzyme in biotechnological applications. In the present study, thermophile Geobacillus sp. EPT3 was isolated from a deep-sea hydrothermal field in the East Pacific. A thermostable superoxide dismutase(SOD) from this strain was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography, and Phenyl-Sepharose chromatography. SOD was purified 13.4 fold to homogeneity with a specific activity of 3 354 U/mg and 11.1% recovery. SOD from Geobacillus sp. EPT3 was of the Mn-SOD type, judged by the insensitivity of the enzyme to both KCN and H2O2. SOD was determined to be a homodimer with monomeric molecular mass of 26.0 k Da. It had high thermostability at 50°C and 60°C. At tested conditions, SOD was relatively stable in the presence of some inhibitors and denaturants, such as β-mercaptoethanol(β-ME), dithiothreitol(DTT), phenylmethylsulfonyl fluoride(PMSF), urea, and guanidine hydrochloride. Geobacillus sp. EPT3 SOD showed striking stability across a wide p H range from 5.0 to 11.0. It could withstand denaturants of extremely acidic and alkaline conditions, which makes it useful in the industrial applications.     

加拿大纽芬兰-拉布拉多省镍矿勘查潜力

纽芬兰-拉布拉多省地处加拿大地盾东缘,成矿条件十分优越,是加拿大重要的镍矿成矿区,矿化类型多样。文章基于前人对该区镍矿床和矿化的研究,从区域成矿地质背景、矿化类型及地质特征、地球物理特征、勘探潜力、勘查态势等等方面进行了系统分析。拉布拉多地区的镍矿资源主要分布于辉长质—橄长质镁铁质岩石分布区,主要与中元古代侵入的辉长岩、斜长岩、橄长岩、辉石岩有关,或与太古宙超镁铁质变火山岩有关,矿化类型有橄长岩和辉长岩型、辉石岩型、斜长岩型、铁闪长岩型、火山岩(科马提岩)型;Voisey′s Bay Ni-Cu-Co硫化物矿床是拉布拉多地区已发现的大型镍矿床。纽芬兰地区的镍矿资源主要与古生代辉长-闪长质侵入岩、前寒武系镁铁质片麻岩有关,矿化类型主要有辉长岩型、镁铁质片麻岩型、火山热液型。纽芬兰-拉布拉多省的镍矿勘查前景乐观。     

Design and Test of 1/5th Scale Horizontal Axis Tidal Current Turbine

Tidal current energy is prominent and renewable. Great progress has been made in the exploitation technology of tidal current energy all over the world in recent years, and the large scale device has become the trend of tidal current turbine (TCT) for its economies. Instead of the similarity to the wind turbine, the tidal turbine has the characteristics of high hydrodynamic efficiency, big thrust, reliable sealing system, tight power transmission structure, etc. In this paper, a 1/5th scale horizontal axis tidal current turbine has been designed, manufactured and tested before the full scale device design. Firstly, the three-blade horizontal axis rotor was designed based on traditional blade element momentum theory and its hydrodynamic performance was predicted in numerical model. Then the power train system and stand-alone electrical control unit of tidal current turbine, whose performances were accessed through the bench test carried out in workshop, were designed and presented. Finally, offshore tests were carried out and the power performance of the rotor was obtained and compared with the published literatures, and the results showed that the power coefficient was satisfactory, which agrees with the theoretical predictions.     

青藏高原地区大地水准面与似大地水准面的转换

研究了联合重力场模型和地形系数模型直接计算大地水准面与似大地水准面差值的方法;分析了影响计算结果精度的误差源。提出利用实际地形数据SRTM3以及密度数据CRUST2.0改善计算结果的建议,并分别进行了比较。青藏高原地区的实际计算结果表明,该地区大地水准面与似大地水准面的差值均值约为0.8 m。     

基于手机信令数据的北京市人口空间分布特征

手机信令数据兼有时间信息和空间信息,是用于研究人口空间分布、城市空间特征较好的大数据。利用手机信令数据和乡镇区划数据,分类分乡镇分析提取不同口径用户数量及用户密度概率分布函数,北京市人口空间分布及其均衡性。研究发现：北京市人口分布呈现出由首都功能核心区向中心城区和新城扩散的态势,对于手机信令可分时段统计用户,呈现出人口密度分布并不平衡,中心城区和新城个别街道如卢沟桥街道、新桥街道、沙河地区等工作居住用户远超于其他乡镇街道,人口聚集明显;在职住方面,个别街道存在就业功能空间外溢,居住功能空间紧张的现象,职住用户分布不匹配现象。     

中国大地构造研究之思考——中国地质学家的责任与担当

19世纪中叶到20世纪中叶盛行的地槽-地台学说,起源于西方学者对大西洋半球的欧洲和北美东部地质的研究。20世纪中叶提出的板块构造学说,虽然起源于对现代海洋的地质、地球物理调查,但用板块学说解释大陆构造的尝试,也是从解剖北美和欧洲大西洋两岸的古生代造山带开始的。二者均缺少太平洋半球的亚洲,特别是中国及邻区的资料基础,因此,其立论基础是不全面的。这就给亚洲,特别是给中国学者提供了很大的发展空间。通过1∶500万国际亚洲地质图的编制和全球构造的对比研究,笔者发现,处于亚洲核心地带的中国及邻区不仅是亚洲也是全球显生宙地质结构和发展历史最复杂的地区。古亚洲洋构造域的乌拉尔—蒙古—兴安巨型造山带是全球规模最大、发育历史最长、地质结构最复杂的古生代造山带;中国西南部,特别是青藏高原是全球特提斯巨型造山带出露宽度最大、地质纪录保存最完整的地段;中国东部的太平洋构造域,既有亚洲东缘的巨型中生代造山带和新生代的沟弧盆体系,又有宽阔的滨太平洋陆缘活化带。这就使中国及邻区成为研究全球显生宙构造不可缺少的重要地段。我们一定要抓住中国在全球构造研究中的区位优势,以地球系统多层圈构造观为指导,用地质、地球物理、地球化学多学科结合的方法,立足实际,抓住特色,构建中国大地构造的新理论、新模型,为国际地球科学,为发展、完善现代大地构造学理论做出应有的贡献!