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1.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
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本文介绍了GDJ跟踪打印经纬仪在人造卫星的观测中如何应用计算机对多圈卫星的目视观测资料进行实时采集、处理等工作过程的原理和方法。 相似文献
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Mercury (Hg) was investigated in bone tissues of skua ( Catharacta maccormick) and penguin (Pygoscelis adeliae) collected in the maritime Antarctic using atomic fluorescence spectrometry (AFS) and synchrotron radiation X-ray fluorescence (SR-XRF) method. The total levels of mercury in bone tissues of penguin and skua are much lower than those in other organs (e. g. , kidney, liver). The toxic effects of mercury in bone tissues of seabirds in polar region are not known. We have used SR- XRF method to map the distribution of trace levels of mercury in bones. The levels of mercury are found to be enriched somewhere near the periosteal surface and/or endosteal surface. The distribution of mercury shows strongly correlation with that of some essential elements and probably poses negative effect on the bone metabolism inferring from the relationship of mercury with the other elements. These studies represent a first step toward understanding the toxic effects of mercury on bone of polar animals by suggesting the possible microscopic investigation. 相似文献
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中国古生教授学会微体古生物学分会举行代表大会和学术年会中国古竽物学会微体古物学分会第五次会员代表大分暨第六次不术年会于1996年1月24日到30日在福州市召开。来自全国地质、石油、煤炭的和产、科研和大学的100多位代表参加了这次大会和学术研讨。大会共... 相似文献
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地壳对海洋潮汐的响应 总被引:2,自引:2,他引:0
应用三维动态有限元方法研究了中国北部地区的地壳对邻近的渤海与黄海海平面变化的响应。虽然此应力场过于微弱不足以引发地震,但发现应力集中的位置及应力场变化较大的位置恰好与某些现代地震的震中一致。这一结果表明研究地壳对广泛分布的载荷的响应对研究区域地震构造是有帮助的。 相似文献
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Zhan-Le Du Hua-Ning Wang Xiang-Tao He National Astronomical Observatories Chinese Academy of Sciences Beijing Department of Astronomy Beijing Normal University Beijing 《中国天文和天体物理学报》2006,6(4):489-494
The maximum amplitudes of solar activity cycles are found to be well anti-correlated (r = -0.72) with the newly defined solar cycle lengths three cycles before (at lag -3) in 13-month running mean sunspot numbers during the past 190 years. This result could be used for predicting the maximum sunspot numbers. The amplitudes of Cycles 24 and 25 are estimated to be 149.5±27.6 and 144.3±27.6, respectively. 相似文献
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Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed. 相似文献
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