Non-specific immune response of bullfrog <Emphasis Type="Italic">Rana catesbeiana</Emphasis> to intraperitoneal injection of bacterium <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis>

Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups. Each bullfrog in bacterium-injected group was injected intraperitoneally （i.p.） with 0.2 ml bacterial suspension at a density of 5.2 × 10^6 CFU/ml, while each one in control group injected i.p. with 0.2 ml sterile saline solution （0.85%, w/v）. Three bullfrogs in both groups were sampled at 0, 1, 3, 7, 11, 15 and 20 days post-injection （dpi） for the evaluation of non-specific immune parameters. It was observed that intraperitoneal injection of A. hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood. Significant increases in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when compared with those in the control group. However, a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes. No significant difference in changes in the number of peripheral erythrocytes, serum superoxide dismutase （SOD） activity, and lysozyme activity was detected between the two groups. It is suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A. hydrophila infection.     

Protective effects of C-phycocyanin on alcohol-induced acute liver injury in mice

Excessive alcohol consumption leads to liver disease. Extensive evidence suggests that C-phycocyanin (C-PC), a chromophore phycocyanobilin derived from Spirulina platensis, exerts protective effects against chemical-induced organ damage. In this study, we investigated whether C-PC could protect against ethanol-induced acute liver injury. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (CHOL), low-density lipoprotein (LDL), liver homogenate malondialdehyde (MDA), superoxide dismutase (SOD) content were measured, and pathological examination of liver sections were examined. C-PC showed obvious inhibitory effects on serum ALT, AST, TG, CHOL, LDL and MDA, and SOD content significantly increased in the liver. The structure of hepatic lobules was clear, liver sinus returned to normal, and liver cell cords were arranged in neat rows. Cloudiness, swelling, inflammatory cell infiltration and spotty necrosis of liver cells were significantly reduced. Therefore, C-PC can significantly protect against ethanol-induced acute liver injury.     

Why chromatic imaging matters

During the last two decades, the first generation of beam combiners at the Very Large Telescope Interferometer has proved the importance of optical interferometry for high-angular resolution astrophysical studies in the near- and mid-infrared. With the advent of 4-beam combiners at the VLTI, the u ? v coverage per pointing increases significantly, providing an opportunity to use reconstructed images as powerful scientific tools. Therefore, interferometric imaging is already a key feature of the new generation of VLTI instruments, as well as for other interferometric facilities like CHARA and JWST. It is thus imperative to account for the current image reconstruction capabilities and their expected evolutions in the coming years. Here, we present a general overview of the current situation of optical interferometric image reconstruction with a focus on new wavelength-dependent information, highlighting its main advantages and limitations. As an Appendix we include several cookbooks describing the usage and installation of several state-of-the art image reconstruction packages. To illustrate the current capabilities of the software available to the community, we recovered chromatic images, from simulated MATISSE data, using the MCMC software SQUEEZE. With these images, we aim at showing the importance of selecting good regularization functions and their impact on the reconstruction.     

Mid-to-Far Infrared Spectral Energy Distribution of Galaxies in the Spitzer First Look Survey Field

We made model fitting to the mid-to-far infrared spectral energy distributions (SEDs) for different categories of galaxies in the main extragalactic field of the Spitzer First Look Survey with the aid of spectroscopic information from the Sloan Digital Sky Survey. We find that the mid-to-far infrared SEDs of HII galaxies, mixture type galaxies and LINERs can be well fitted by the one-parameter (α) dust model of Dale et al. plus the 13 Gyr dust-free elliptical galaxy model. The statistics of α values indicates that all these galaxies tend to be quiescent, although the HII galaxies are relatively more active than the LINERs. The mid- infrared SEDs of absorption galaxies are well fitted simply by the 13 Gyr dust-free elliptical galaxy template, and the near-to-mid infrared SEDs of QSOs can be represented by AGN NGC 5506.     

EFFECTS OF FISH OIL,DHA OIL AND LECITIHIN IN MICROPARTICULATE DIETS ON STRESS TOLERANCE OF LARVAL GILTHEAD SEABREAM（SPARUS AURATA）

The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin.     

南极普里兹湾附近海域表层海流特征分析

利用第29次南极科考期间在普里兹湾海区释放的8个Argos漂流浮标所获的总计662 d的数据,对普里兹湾及其邻近海域的表层海流特征进行了分析,并和以前的研究结果进行对比,结果表明:普里兹湾西部和北部表层海流基本呈现向西的流动,说明此地区的表层海流以沿南极大陆的逆时针沿岸流为主。普里兹湾东部海流呈现先向南再向西或先向南再向北的流动,普里兹湾西北部海流呈现向南的流动。8个浮标平均流速在0.02—0.20 m·s~(-1)之间,最大流速为1.57 m·s~(-1),流速1.0 m·s~(-1)的大流速区主要集中在普里兹湾、克洛斯角外侧、达恩利角外侧海域,流速1.5 m·s~(-1)的大流速区只出现在普里兹湾、克洛斯角外侧两处海域。     

西南印度洋深海热液硫化物区沉积物微生物群落结构和固氮基因多样性

A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction（PCR） amplification of the 16 S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E（MBGE） and marine group I（MGI） belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilonproteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the Southwest Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III NifH were present. This is consistent with the phylogenetic analysis of the microbial 16 S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.     

Report on Pontoniinae shrimps （Crustacea：Decapoda）collected by Joint Chinese—German Marine Biology Expeditions to Hainan lsland ,South China Sea：Ⅱ.Harpiliopsis,Ischnopontonia,Jocaste,Palae—mone

The present paper reports 9 species of pontoniine shrimps collected from Hainan IS-land ,South China Sea ,by the Joint Chinese-German Marine Biology Expeditions,in which Peri-climenaeus arabicus (Calman,1939) and Periclimenaeus hecate (Nobili,1904) are recorded for the first time from Hainan Island.     

Total N,total P and organic matters content in floodplain soils of Xianghai Nature Reserve

Soil sediment samples of 10 layers with a spacing of 10 cm each were collected in different floodplain zones adjacent to Huolin River in the Xianghai Nature Reserve, and contents of total N, total P and organic matters were analyzed. The results showed that contents of total N, total P and organic matters were generally decreasing with the increase of distance from sample locations to the river channel, and contents of the three items were generally higher in the upper soil layer than that in the lower soil layer. The content variations displayed how flooding functions influenced nutrient matter content variations in floodplain soils since the flood inundation frequencies of the sample locations varied. The correlation analysis displayed that there were remarkable relativities between total N, total P and organic matters within definite spatial distance from the Huolin River channel.     

Gravity anomaly interpretation of 2D fault morphologies by means of nonplanar fault planes and exponential density contrast model: a space domain technique

Boundary faults associated with thick sedimentary basins are more often curved in cross section rather than planar. We develop a space domain-based automatic gravity inversion technique to quantify such listric fault sources from a set of observed gravity anomalies. The density contrast within the hanging wall of fault morphology is presumed to be known according to a prescribed exponential law. Furthermore, the fault plane is described by a polynomial function of arbitrary but specific degree, whose coefficients become the unknown parameters to be estimated from a set of observed gravity anomalies in addition to the thickness of the fault structure. Using a set of characteristic anomalies, the present inversion identifies approximate parameters pertaining to the origin of fault plane and depth to decollement horizon. Based on the errors between the observed and model gravity anomalies of the structure, the algorithm constructs and solves a system of normal equations to estimate the improvements in depth and coefficients of the polynomial in an iterative approach until one of the specified convergence criteria is fulfilled. The efficacy of the algorithm is shown with the analysis of gravity anomalies attributable to a synthetic model of a listric fault source in the presence of pseudorandom noise. Application of the proposed inversion technique on the observed gravity anomalies of the Ahri-Cherla master fault of the Godavari subbasin in India using the derived exponential density contrast model has yielded an interpretation that is consistent with the available/reported information.