In crustaceans, the male sexual differentiation and maintenance are specially regulated by androgenic gland (AG). However, little is known about the genes involved in the regulation process. RNA-Seq was performed on AG with ejaculatory duct (AG_ED) and ejaculatory duct (ED) as control in Eriocheir sinensis, one of the most important economic and fishery crabs with typically sex dimorphism. A total of 925 unigenes were identified as differentially expressed genes (DEGs) and the expression of nine genes randomly selected was confirmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of insulin-like androgenic gland factor (IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes insulin-like receptor (INR), and single insulin binding domain protein (SIBD), might constitute the sex regulation pathway. Several sex related genes were identified, and their function will have to be investigated. Also, the identification of juvenile hormone epoxide hydrolase 1 (JHEH1), ecdysteroid 22-hydroxylase (DIB) and ecdysone receptor (ECR) preliminarily clarified the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.
In this study,the activated sludge model implemented in the BioWin software was validated against full-scale wastewater treatment plant data. Only two stoichiometric parameters(Y p /acetic and the heterotrophic yield(Y H)) required calibration. The value 0.42 was used for Yp /acetic in this study,while the default value of the BioWin software is 0.49,making it comparable with the default values of the corresponding parameter(yield of phosphorus release to substrate uptake(YPO4)) used in ASM2,ASM2 d,and ASM3 P,respectively. Three scenarios were evaluated to improve the performance of the wastewater treatment plant,the possibility of wasting sludge from either the aeration tank or the secondary clarifier,the construction of a new oxidation ditch,and the construction of an equalization tank. The results suggest that construction of a new oxidation ditch or an equalization tank for the wastewater treatment plant is not necessary. However,sludge should be wasted from the aeration tank during wet weather to reduce the solids loading of the clarifiers and avoid effluent violations. Therefore,it is recommended that the design of wastewater treatment plants(WWTPs) should include flexibility to operate the plants in various modes. This is helpful in selection of the appropriate operating mode when necessary,resulting in substantial reductions in operating costs. 相似文献
To further investigate the influence of cloud base temperature, updraft velocity and precipitation particle constitution on cloud electrification, five thunderstorms in various regions of China were simulated by using the three-dimensional compressible hailstorm numerical model including inductive and non-inductive charging mechanisms. The results indicate that changes of cloud base temperature have an influence on the initial electrification. Comparison of the above cases shows that in the case of warm cloud base and moderate updraft velocity (< 20 m s− 1), active electrification occurred below the − 10 °C level before moving upward to the − 20 °C level. In contrast, when cloud base is cold and updraft velocity is intensive, the main charging region is at the − 20 °C or even higher level. In that case, the vertical extent of the main negative charge region becomes larger with the increase of cloud base temperature. Apart from the main dipolar or tripolar charge structure, some smaller charge regions with relatively high values of charge density may also appear. Frozen drops, originating mainly from supercooled raindrops, mainly get electrified through charging interactions with snow at or below the − 20 °C level. They are responsible for the negative charge region near the melting level at the initial stage of precipitation if there is a large supercooled raindrop content. Non-inductive charging during hail-snow collisions is rather weak, resulting in the charge density on hail of no more than − 0.01 nC m− 3. 相似文献
The Florida peninsula in the USA has a frequent occurrence of sea breeze(SB)thunderstorms.In this study,the numerical simulation of a Florida SB and its associated convective initiation(CI)is simulated using the mesoscale community Weather Research and Forecasting(WRF)model in one-way nested domains at different horizontal resolutions.Results are compared with observations to examine the accuracy of model-simulated SB convection and factors that influence SB CI within the simulation.It is found that the WRF model can realistically reproduce the observed SB CI.Differences are found in the timing,location,and intensity of the convective cells at different domains with various spatial resolutions.With increasing spatial resolution,the simulation improvements are manifested mainly in the timing of CI and the orientation of the convection after the sea breeze front(SBF)merger into the squall line over the peninsula.Diagnoses indicate that accurate representation of geophysical variables(e.g.,coastline and bay shape,small lakes measuring 10-30 km2),better resolved by the high resolution,play a significant role in improving the simulations.The geophysical variables,together with the high resolution,impact the location and timing of SB CI due to changes in low-level atmospheric convergence and surface sensible heating.More importantly,they enable Florida lakes(30 km2 and larger)to produce noticeable lake breezes(LBs)that collide with the SBFs to produce CI.Furthermore,they also help the model reproduce a stronger convective squall line caused by merging SBs,leading to more accurate locations of postfrontal convective systems. 相似文献
An episode of persistent wintertime inversion over Salt Lake City, Utah and its vicinity from 1200 UTC 30 November to 0000 UTC 7 December 2010 is simulated using an advanced research version of the Weather Research and Forecasting model. The numerical simulations agree well with observed soundings in temperature, wind speed, and wind direction in the atmospheric boundary layer and above, although there are some differences near the surface due to the influence of complex terrain in the area. The characteristics of large-scale environmental conditions and their interactions with local-scale processes are analyzed to understand the factors that influence the onset and evolution of persistent inversions. It is found that the inversion formed mainly because of the interaction between the heating effect from a high-pressure ridge in the mid-troposphere and a near-surface cold pool due to the effects of radiation. During the following six to seven days, the high-pressure ridge was maintained and vertical motion very weak, allowing a persistent inversion to become established. Finally, the cold effect from a low-pressure trough in the mid-troposphere, combined with mixing due to vertical motion, led to extreme weakening of the persistent inversion. 相似文献
Biogeophysical effects of land use and land cover (LULC) changes play a significant role in modulating climate on various spatial scales. In this study, a set of recent LULC products with a spatial resolution of 500 m was developed in China for update in RegCM4 (regional climate model version 4). Two sets of comparative numerical experiments were conducted to study the effects of LULC changes on near-surface temperature simulation. The results show that after LULC changes, areas of crops and mixed woodlands as well as urban areas increase over entire China, accompanied with greatly expanded mixed farming and forests/field mosaics in southern China, and reduced areas of 1) irrigated crops and short grasses in northern China and the Tibetan Plateau, and 2) semi-desert and desert in northwestern China. Improvements in the LULC data clearly result in more accurate simulations of the near-surface temperature. Specifically, increasing latent heat and longwave albedo due to enhanced LULC in certain areas lead to reduction in land surface temperature (LST), while changes in shortwave albedo and sensible heat also exert a great influence on the LST. Overall, these parameter adjustments reduce the biases in near-surface temperature simulation. 相似文献
This study investigated the performance of the mesoscale Weather Research and Forecasting (WRF) model in predicting near-surface atmospheric temperature and wind for a complex underlying surface in Northwest China in June and December 2015. The spatial distribution of the monthly average bias errors in the forecasts of 2-m temperature and 10-m wind speed is analyzed first. It is found that the forecast errors for 2-m temperature and 10-m wind speed in June are strongly correlated with the terrain distribution. However, this type of correlation is not apparent in December, perhaps due to the inaccurate specification of the surface albedo and freezing–thawing process of frozen soil in winter in Northwest China in the WRF model. In addition, the WRF model is able to reproduce the diurnal variation in 2-m temperature and 10-m wind speed, although with weakened magnitude. Elevations and land-use types have strong influences on the forecast of near-surface variables with seasonal variations. The overall results imply that accurate specification of the complex underlying surface and seasonal changes in land cover is necessary for improving near-surface forecasts over Northwest China. 相似文献