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311.
Guoyan Ren Bafang Li Xue Zhao Yongliang Zhuang Mingyan Yan Hu Hou Xiukun Zhang Li Chen 《中国海洋大学学报(英文版)》2009,8(1):83-88
In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified
target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted
against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions
containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted
water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q
water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method
was the optimum extraction method selected by HPLC. 相似文献
312.
济南高新区的破损山体治理任务涉及5个山头126万m2破损山体的治理,主要分布在经十东路和旅游路奥体中心附近.十一届全运会2009年10月在济南召开,按照省、市委的要求,2007年6月份,济南高新区拉开了整治破损山体的工作序幕. 相似文献
313.
We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future. 相似文献
314.
This study utilizes the 62208000 Swarm satellite data to establish a high-precision main magnetic field at the height of the satellites in China and its adjacen... 相似文献
315.
Zhi-Jia Tian Xiao-Wei Liu Hai-Bo Yuan Bing-Qiu Chen Mao-Sheng Xiang Yang Huang Chun Wang Hua-Wei Zhang Jin-Cheng Guo Juan-Juan Ren Zhi-Ying Huo Yong Yang Meng Zhang Shao-Lan Bi Wu-Ming Yang Kang Liu Xian-Fei Zhang Tan-Da Li Ya-Qian Wu Jing-Hua Zhang 《天文和天体物理学研究(英文版)》2018,(5)
Stellar systems composed of single, double, triple or higher-order systems are rightfully regarded as the fundamental building blocks of the Milky Way. Binary stars play an important role in formation and evolution of the Galaxy. Through comparing the radial velocity variations from multiepoch observations, we analyze the binary fraction of dwarf stars observed with LAMOST. Effects of different model assumptions, such as orbital period distributions on the estimate of binary fractions,are investigated. The results based on log-normal distribution of orbital periods reproduce the previous complete analyses better than the power-law distribution. We find that the binary fraction increases with Teff and decreases with [Fe/H]. We first investigate the relation between α-elements and binary fraction in such a large sample as provided by LAMOST. The old stars with high [α/Fe] dominate with a higher binary fraction than young stars with low [α/Fe]. At the same mass, earlier forming stars possess a higher binary fraction than newly forming ones, which may be related with evolution of the Galaxy. 相似文献
316.
Humans and livestock have always dwelling together, especially in rural areas. Based on a multi-method approach of ethnographic fieldwork, grounded theory and one-way multivariate analysis of variance, this paper explores human and livestock dwelling. The findings show that human and livestock dwelling form a special type of human dwelling place, with forms defined as co-, mix- and dis-dwelling. Then, from co-, mix- to dis-dwelling constructed a story line to the evolution of human and livestock dwelling forms, which further explains the entire dwelling situation of humans and livestock, including the dimensions of human dweller, livestock dweller, the rural environment and special events. Among co-, mix- and dis-dwelling significant differences existed between human and livestock individuals, the nature of rural environment, vernacularity and rural tourism development. Between co- and dis-dwelling, significant differences existed in seventeen aspects; between mix- and dis-dwelling, significant differences existed in eleven aspects; meanwhile, no significant differences existed between co- and mix-dwelling. Accordingly, this suggested that human and livestock dwelling forms can be divided into mix- and dis-dwelling. This study on humans and livestock dwelling relationship offer a new perspective on rural and animal geography. This paper is an exploratory foray into rural dwelling geography as part of the Anthrozoology study and broadens the scope of extant scholarship on human dwelling morphology, as well as analyses the spatial relationships between human and livestock dwelling. 相似文献
317.
Journal of Oceanology and Limnology - Genes encoding Wnt ligands, which have important roles in cell communication and organ development, are restricted to multicellular animals. We systematically... 相似文献
318.
319.
Based on the analysis and study of mineral composition, structure, and major and trace elements, this paper deals with element geochemical characteristics and transformation from primary ore into oxidized ore in the Erzhe gold deposit, Sichuan. Some grounds are provided to constrain the transformation of primary ore to oxidized ore. 相似文献
320.