In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns
were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective
form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized
methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally
suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard-
Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K
(1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting
DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from
glacier surface snow. 相似文献
Land use change is one of the important aspects of global change[1-3]. The Bohai Rim is a hot spot of economic development in China, where land use changes remarkably. Analyzing land use change in this area is significant to the research of global change and regional sustainable land use. Although there has been much work undertaken on regional land use change in China, it was mainly on urban land use change[4-11], and few researches integrated remote sensing and GIS method. Based on data f… 相似文献
A field experiment was conducted from 2 May 2010 to 1 May 2012 in the Gurbantunggut Desert, the second largest desert in China, to investigate saltation activity and its threshold velocity, and their relations with atmospheric and soil conditions. The results showed that saltation activity occurred more frequently during 08:00–20:00 Local Standard Time in spring and summer, with air temperatures between 20.0 and 29.0 °C, water vapor pressures between 0.6 and 0.9 kPa, soil temperatures between 25.0 and 30.0 °C, and a soil moisture lower than 0.04 m3/m3. At 2 m height, the saltation threshold velocity varied between 11.1 and 13.9 m/s, with a mean of 12.5 m/s. Threshold velocity showed clear seasonal variations in the following sequence: spring (11.7 m/s) < autumn (12.7 m/s) < summer (13.6 m/s). Affected by soil conditions, aeolian sand transport was weak, with an average annual aeolian sand that transported across a section (1.0 m × 2.0 m) of less than 6.0 kg. 相似文献