基于全基因组重测序技术的浙江近岸海域耐盐金黄色葡萄球菌(Staphylococcus aureus)耐药机制解析<

金黄色葡萄球菌(Staphylococcus aureus)是近岸海域海水中的主要病原菌,严重威胁接触者的安全。抗生素处理是治疗金黄色葡萄球菌感染的重要手段,其耐药性的发生受到了高度重视。采用全基因组重测序与KEGG富集分析结合的方法,对红霉素(erythromycin)、氯霉素(chloramphenicol)和万古霉素(vancomycin)处理后的耐盐金黄色葡萄球菌(S.aureus ZS01)和不耐盐金黄色葡萄球菌(S.aureus 502A)进行耐药机制研究。结果表明,S.aureus 502A经抗生素处理后发生突变的程度大于S.aureus ZS01,二者在经过氯霉素处理发生了更大程度的突变。红霉素、氯霉素和万古霉素处理主要影响了金黄色葡萄球菌的致病能力;红霉素和氯霉素可能通过影响金黄色葡萄球菌脂类的代谢引起其耐药性的变化。除此之外,三种抗生素处理均出现了较多TIGR01741家族蛋白和假设蛋白基因的突变,推测与菌株的耐药性和致病性相关。耐盐金黄色葡萄球菌可通过外排系统作用产生红霉素耐药性,不耐盐菌株因细胞壁成分相关基因的突变提高了对万古霉素的耐受性。研究结果可为耐盐和不耐盐金黄色葡萄球菌的耐药机制及抗生素对金黄色葡萄球菌致病性影响的研究提供基础数据。     

利用MAPINFO建立渤海区域地质信息管理系统空间数据库的技术方案

以渤海区域地质信息管理系统空间数据库为例,概述了利用地理信息工具平台MAPINFO建设渤海信息管理系统区域地质空间数据库的方法,包括空间数据库的分类、分层方案,命名及编码规则,以及利用MAPINFO和VERTICAL MAPPER绘制水深等值线图、剖面图、3D图的技巧。     

洞庭湖镉迁移转化的马尔可夫模型评价

讨论了在现时泥沙、水体、鱼类、藻类的含镉状态下洞庭湖的输入输出通量；并以其为例，在简要介绍马尔可夫链数学方法的基础上，建立了湖泊生态系统中镉迁移转化的马尔可夫链数学模型，初步评价了当洞庭湖不断有镉加入、系统到达状态稳定时各种介质中的镉总量及镉的迁移转化趋势。     

土水特征曲线(SWCC)的滞回特性模拟研究

为了模拟非饱和土的水力、力学特性在多次降雨、蒸发过程中的变化特性,本文以传统域模型的基本原理为基础,推到得到了一个能够模拟SWCC滞后性的计算模型,该模型计算方法简单,易于通过程序实现,且通过与试验数据和其他模型的计算结果对比,验证了该模型的合理性,同时,这个模型可为研究在复杂吸力变化状态下非饱和土的渗流特性、强度特性以及本构模型的研究打下一定的基础。     

The Coarse-Grained Lacustrine Slope Apron Deposits in the Moliqing Area, Yitong Basin, Northeast China

Overlapping gravity accumulation bodies were formed on the northwestern steep slope of the Shuangyang Formation in the Moliqing fault depression of northeast China. This study analyzed in detail the spatial distribution of the lithofacies and lithofacies associations of these accumulation bodies based on more than 600 m of core sections, and summarized 12 major types of lithofacies and three types of lithofacies associations: (1) the proximal zone consists of gravelly debris flows dominated by alluvial channel conglomerates; (2) the middle zone is dominated by various gravity flow deposits and traction flow deposits; and (3) the distal zone is dominated by mudstones with intercalations of sandy debris and turbidites. Combining with the grain size cumulative probability curves analysis, we determined the transformation of debris flows to sandy debris flows and to turbidity currents in the slope zone of the basin margin, and further proposed a lacustrine slope apron model that is characterized by (1) an inconstant multiple source (line source), (2) an alternation of gravity flow deposits and traction flow deposits dominated by periodical changes in a source flood flow system, and (3) the transformation of sandy debris flow deposits into distal turbidity current deposits. This sedimentary model may be applicable to other fault depressions for predicting reservoir distribution.     

A10/35绝对重力仪测试及性能分析

A10型绝对重力仪用于测量重力加速度(g),具有高精度、便携式和易操作等优点。介绍了A10的基本原理,并与FG5型重力仪进行了对比分析。基于A10/35绝对重力仪的室内和野外测量数据,分析了该仪器的稳定性和影响测量精度的主要因素。测试结果表明,不同环境下该仪器测量组间离散度均小于10×10-8ms-2,仪器性能稳定可靠。最后提出了实际使用的具体建议。     

Gaussian decomposition and component pigment spectral analysis of phytoplankton absorption spectra

The absorption spectrum of phytoplankton is an important bio-optical parameter for ocean color hyperspectral remote sensing;its magnitude and shape can be affected considerably by pigment composition and concentration.We conducted Gaussian decomposition to the absorption spectra of phytoplankton pigment and studied the spectral components of the phytoplankton,in which the package effect was investigated using pigment concentration data and phytoplankton absorption spectra.The decomposition results were compared with the corresponding concentrations of the five main pigment groups(chlorophylls a,b,and c,photo-synthetic carotenoids(PSC),and photo-protective carotenoids(PPC)).The results indicate that the majority of residual errors in the Gaussian decomposition are0.001 m~(-1),and R~2 of the power regression between characteristic bands and HPLC pigment concentrations(except for chlorophyll b)was0.65 or greater for surface water samples at autumn cruise.In addition,we determined a strong predictive capability for chlorophylls a,c,PPC,and PSC.We also tested the estimation of pigment concentrations from the empirical specific absorption coefficient of pigment composition.The empirical decomposition showed that the Ficek model was the closest to the original spectra with the smallest residual errors.The pigment decomposition results and HPLC measurements of pigment concentration are in a high consistency as the scatter plots are distributed largely near the 1:1 line in spite of prominent seasonal variations.The Wozniak model showed a better fit than the Ficek model for Chla,and the median relative error was small.The pigment component information estimated from the phytoplankton absorption spectra can help better remote sensing of hyperspectral ocean color that related to the changes in phytoplankton communities and varieties.     

海上设施自动化系统软件认可流程与技术要求研究

随着计算机及通信技术的发展,海上设施自动化水平的不断提高,海上设施运营的安全可靠性越来越依赖于自动控制系统,尤其是软件部分。由于软件本身在设计和开发中可能存在的缺陷引起系统故障或失效,将给设施及利益方带来不可估量的损失。文章结合IEC61508系列标准、ISO9000—3标准,针对自动化系统软件有关要求和国际船级社协会及船级社等机构有关规范和标准的内容进行分析研究,系统地分析总结出海上设施软件的认可流程及对应技术要求,为我国海工装备设计制造业提供指导。     

大型地面太阳望远镜选址云量调查

利用中国756个地面基本、基准气象站1961～2008年云量年值、月值数据集,分析全国地面太阳望远镜选址的云量条件,结果表明;40°N纬度带和藏西南地区,云量条件最优;其次是新疆西部和北部、东北北部和东部、青藏高原南部拉萨、江孜一带;西藏东部、四川西部及云南大部,云量条件居中;33° N以南,包括江南华南、青藏高原东南部林芝附近及四川盆地、重庆、贵州一带,云量条件较差.     

应用计算机进行固体矿产储量计算,提高地质工作水平

Windos XP版储量计算程序是作者90年代DOS版的全面升级。该程序按照目前地质工作普遍采用的公式和做法,符合现行规范规定和要求。程序对储量计算中矿体圈定等难点解决较好。该程序已经多个矿区应用检验,最近又为南非英铂公司采用推广。