茂名大放鸡海域文昌鱼、蛇尾分布及其与底质粒度特征的关系

为给茂名市建设文昌鱼省级自然保护区提供基础资料,根据2003年8月～2004年5月间对茂名大放鸡海域的调查结果,对文昌鱼和蛇尾的分布特性进行了研究.结果表明:文昌鱼和蛇尾均呈集群分布,在空间分布上表现为正相关关系,不存在强烈的竞争关系.文昌鱼主要分布在中值粒径MdΦ为0.4～2.2之间砂质中.在0.5≤MdΦ＜1.5范围内,文昌鱼分布密度相对地高,而在MdΦ＜0.5或MdΦ≥1.5范围内,文昌鱼分布密度相对较低.粒径0.36～0.78 mm组分＜50%时,文昌鱼分布密度≤50 ind/m2;粒径0.36～0.78 mm组分≥50%时,文昌鱼分布密度＞100 ind/m2.蛇尾分布在中值粒径MdΦ为0.2～0.8之间砂质中.粒径 0.36～0.78 mm组分占10%～50%时,蛇尾分布密度较低,而粒径0.36～0.78 mm组分占60%～80%时,蛇尾分布密度较高.     

运用荧光显微技术分析北黄海夏季浮游病毒的分布

运用荧光显微技术(Epifluorescence Microscopy,EFM),对2006年夏季北黄海水域31个站点的病毒丰度进行了检测,对浮游病毒丰度在水平分布,垂直分布和昼夜变化上进行了探讨.北黄海水域浮游病毒直接检测量(Virus Direct Count,VDC)为(1.58×106～1.38×107)/mL,平均为5.86×106/mL.在水平分布上,表层、30 m和底层水在辽东半岛头部附近出现了病毒较高区域,近海水域的病毒丰度较中部水域高(P＜0.05).在垂直分布上,表层浮游病毒丰度高于30 m水层和底层(P＜0.05).在昼夜变化上,表层水体中浮游病毒量有明显的变化,10 m水层和30 m水层的病毒丰度昼夜变化较明显,底层病毒丰度昼夜趋势平缓.利用多元相关分析可知,北黄海夏季浮游病毒丰度与站位总水深(P＜0.01)和盐度(P＜0.05)成一定的负相关性,与温度无明显相关性(P＞0.05).     

一种水不溶灰树花菌丝体多糖(GF4A)硫酸酯化研究

以灰树花发酵菌丝体水不溶性多糖GF4A为原料,以三氧化硫三甲胺盐(SO3·Me3N)为酯化试剂,探讨了GF4A的硫酸酯化工艺.实验结果表明,当三氧化硫与GF4A中单糖摩尔比为5.8∶1、反应温度为80 ℃、反应时间6 h时,所得酯化产物(SGF4A)水溶性好、产率高.该产物硫酸酯取代度为1.6, 相对分子质量为88 kD.通过红外光谱及核磁共振碳谱分析表明,糖残基中所有C-6羟基以及部分C-4和C-2位羟基被酯化.体外实验表明,SGF4A具有较好的抗凝活性.     

高频电力线信息传输的电机模型与仿真

研究在电机驱动系统中,电力线通信的建模问题,着重讨论在高频时电机输入阻抗的特性下,电机模型的建立.通过实验测量得到电机和电力线在频率100 kHz～40 MHz的输入阻抗特性曲线,并分析高频输入阻抗的谐振特点.在Matlab中,提出基于阻抗公式的分析法、最小均方值误差估计法以及最优化方法进行模型的参数识别,将得到的参数代入模型中可以得到电机的阻抗模型.电机模型的精确度可以通过仿真曲线和测量曲线的逼近程度看出,从结果看仿真得到的阻抗曲线跟实际测量的阻抗曲线走向基本一致.     

Construction of bacterial artificial chromosome libraries for Zhikong Scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis>

Two Large-insert genomic bacterial artificial chromosome （BAC） libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.     

Pb distribution and translocation in Jiaozhou Bay

The trends of distribution, translocation and seasonal change of heavy metal Pb were studied based on the surface and bottom water sampling in Jiaozhou Bay in 1979, and compared with those in 1990＇s. The results showed that the source of Pb in the bay was from wastewater and sewage in the east of Jiaozhou Bay from ocean vessels. Pb concentration was higher in spring and lower in summer and autumn, and remained stable through sedimentation in the bottom layer. The overall water quality was good in 1970＇s. Compared with the environmental monitoring data of 1995-1999, Pb pollution had become serious. Therefore, more efforts should be made to protect the bay from Pb pollution.     

Rossby waves with linear topography in barotropic fluids

Rossby waves are the most important waves in the atmosphere and ocean, and are parts of a large-scale system in fluid. The theory and observation show that, they satisfy quasi-geostrophic and quasi-static equilibrium approximations. In this paper, solitary Rossby waves induced by linear topography in barotropic fluids with a shear flow are studied. In order to simplify the problem, the topography is taken as a linear function of latitude variable y, then employing a weakly nonlinear method and a perturbation method, a KdV （Korteweg-de Vries） equation describing evolution of the amplitude of solitary Rossby waves induced by linear topography is derived. The results show that the variation of linear topography can induce the solitary Rossby waves in barotropic fluids with a shear flow, and extend the classical geophysical theory of fluid dynamics.     

Dynamics and relationships of Ca, Mg, Fe in litter, soil fauna and soil in Pinus koraiensis-broadleaf mixed forest

The Liangshui Natural Reserve in Heilongjiang Province of China was selected as the study area. The authors collected the samples of forest litter （Tilia amurensis, Fraxinus mandshurica, Pinus koraiensis, Acer mono, Betula costata, and mixed litter）, soil in humus horizon （0--5cm） and soil horizon （5-20cm）, and soil macrofauna （Oligochaeta, Geophiloporpha and Juliformia） from 2001 to 2002. The role of soil macrofauna in the material cycle was analyzed through comparing the macro-element contents among various parts of the subsystems and using enrichment index （El）. The results indicate that dynamic changes of various litters are very complicated. The contents of Fe in each kind of litter increase firstly, and then decrease in the study period. The changes of macro-element contents are greater in the broad-leaf litter than in the coniferous litter, and the mixed litter is in the middle level, but the differences among them are not significant. The contents of Mg and Fe in humus are higher than those in soil, but the contents of Ca in soil are higher than that in humus. The dynamic changes of macro-element contents in soil and soil fauna are not consistent with those in litter. The diplopod presented obvious enrichment of Ca and Mg （E1〉1）, but it does not significantly enrich Fe. Earthworm has a stronger enrichment ability of Fe than diplopod and scolopendra, but E1〈1. Soil fauna can make great influences on the material cycle of the subsystems.     

The hydrogen peroxide impact on larval settlement and metamorphosis of abalone <Emphasis Type="Italic">Haliotis diversicolor supertexta</Emphasis>

Abalone Haliotis diversicolor supertexta is an important economic mollusk. The settlement and metamorphosis are two critical stages during its development period, which has direct influence on abalone survival and production. The influence of reactive oxygen species （hydrogen peroxide） on abalone embryo and juvenile development were examined in this study. Larvae of Haliotis diversicolor supertexta were induced to settlement and metamorphose by exposure to seawater supplemented with hydrogen peroxide. They had the best performance at 800 μmol/L. The concentration of 1 000 μmol/L or higher was toxic to the larvae, as the larvae could settle down only at benthic diatom plates without complete metamorphosis. In addition, H2O2 adding time was critical to the larval performance. 24h after two-day post-fertilization was proved to be the optimal adding time. In this paper, two action mechanisms of hydrogen peroxide are discussed： （1） hydrogen peroxide has direct toxicity to ciliated cells, thus cause apoptosis; （2） hydrogen peroxide, as a product from catecholamines＇ autoxidation process in vivo, can reverse this process to produce neuro-transmitters to induce abalone metamorphosis.