Rotation Center and Horizontal Bearing Capacity of the Bucket Foundation in Soft Ground

In the last 20 years, the bucket foundation has been developed as a new type of offshore platform structure. Because of its short period of application to engineering practices, the theoretical decision on the rotation center and horizontal bearing capacity of the bucket foundation has yet to be agreed. A limit analysis method is used to determine the updated rotation center position and horizontal bearing capacity to evaluate the failure mechanisms of the bucket foundations. The results are compared with numerical simulation and experiments, and also with other theoretical methods. The proposed method can satisfactorily consider the engineering conditions and the result is accurate in determining the rotation center and horizontal bearing capacity.     

Seasonal variation and principle of cyanobacterial biomass and forms in the water source area of Chaohu City,China

We investigated seasonal variations in cyanobacterial biomass and the forms of its dominant population(M. aeruginosa) and their correlation with environmental factors in the water source area of Chaohu City,China from December 2011 to October 2012. The results show that species belonging to the phylum Cyanophyta occupied the maximum proportion of phytoplankton biomass,and that the dominant population in the water source area of Chaohu City was M. aeruginosa. The variation in cyanobacterial biomass from March to August 2012 was well fitted to the logistic growth model. The growth rate of cyanobacteria was the highest in June,and the biomass of cyanobacteria reached a maximum in August. From February to March 2012,the main form of M.aeruginosa was the single-cell form; M.aeruginosa colonies began to appear from April,and blooms appeared on the water surface in May. The maximum diameter of the colonies was recorded in July,and then gradually decreased from August. The diameter range of M. aeruginosa colonies was 18.37–237.77 μm,and most of the colonies were distributed in the range 20–200 μm,comprising 95.5% of the total number of samples. Temperature and photosynthetically active radiation may be the most important factors that influenced the annual variation in M. aeruginosa biomass and forms. The suitable temperature for cyanobacterial growth was in the range of 15–30°C. In natural water bodies,photosynthetically active radiation had a significant positive influence on the colonial diameter of M.aeruginosa(P0.01).     

Identification and bioinformatics analysis of microRNAs from the sporophyte and gametophyte of <Emphasis Type="Italic">Pyropia haitanensis</Emphasis>

Pyropia haitanensis (T. J. Chang et B. F. Zheng) N. Kikuchi et M. Miyata (Porphyra haitanensis) is an economically important genus that is cultured widely in China. P. haitanensis is cultured on a larger scale than Pyropia yezoensis, making up an important part of the total production of cultivated Pyropia in China. However, the majority of molecular mechanisms underlying the physiological processes of P. haitanensis remain unknown. P. haitanensis could utilize inorganic carbon and the sporophytes of P. haitanensis might possess a PCK-type C₄-like carbon-fixation pathway. To identify microRNAs and their probable roles in sporophyte and gametophyte development, we constructed and sequenced small RNA libraries from sporophytes and gametophytes of P. haitanensis. Five microRNAs were identified that shared no sequence homology with known microRNAs. Our results indicated that P. haitanensis might posses a complex sRNA processing system in which the novel microRNAs act as important regulators of the development of different generations of P. haitanensis.     

Strategy of photo-protection in phytoplankton assemblages in the Kongsfjorden,Svalbard, Arctic

Photo-protective functions were investigated in phytoplankton assemblages at Kongsfjorden, Svalbard in spring, using their UV-absorbing compounds (mycosporine-like amino acids (MAAs)), xanthophyll pigments (diadinoxanthin (DD) and diatoxanthin (DT)) and < beta >- dimethylsulphoniopropionate (< beta >_-DMSP). The dominant phytoplankton species in the inner bay were dominated by Phaeocystis spp. and nanoflagellates, while the offshore waters were dominated by Thalassiosira spp. In the inner bay, UVabsorbing compounds and xanthophyll pigments exhibited higher ratios of MAA to chlorophyll a (MAA:chl a ratio), and both DD and DT to chlorophyll a (DD:chl a ratio and DT:chl a ratio), respectively. Thus, the photoprotective-pigments such as DD and DT appear to complement MAAs in the natural phytoplankton assemblage. However, the ratio of < beta >_-DMSP to chlorophyll a (< beta >_-DMSP:chl a ratio) did not show a distinct spatial distribution according to environmental factors or interspecies differences. In this study, we found that photoprotective compounds occurred in a manner dependent on the phytoplankton species composition in Kongsfjorden Bay, where Phaeocystis is the dominant species.     

Protective effects of C-phycocyanin on alcohol-induced acute liver injury in mice

Excessive alcohol consumption leads to liver disease. Extensive evidence suggests that C-phycocyanin (C-PC), a chromophore phycocyanobilin derived from Spirulina platensis, exerts protective effects against chemical-induced organ damage. In this study, we investigated whether C-PC could protect against ethanol-induced acute liver injury. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (CHOL), low-density lipoprotein (LDL), liver homogenate malondialdehyde (MDA), superoxide dismutase (SOD) content were measured, and pathological examination of liver sections were examined. C-PC showed obvious inhibitory effects on serum ALT, AST, TG, CHOL, LDL and MDA, and SOD content significantly increased in the liver. The structure of hepatic lobules was clear, liver sinus returned to normal, and liver cell cords were arranged in neat rows. Cloudiness, swelling, inflammatory cell infiltration and spotty necrosis of liver cells were significantly reduced. Therefore, C-PC can significantly protect against ethanol-induced acute liver injury.     

Importance of kelp-derived organic carbon to the scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis> in an integrated multi-trophic aquaculture system

Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture (IMTA) systems. A beneficial relationship between seaweed and bivalve in the seaweed-based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharina japonica to the co-cultured scallop Chlamys farreri in a typical IMTA farm in Sungo Bay (Weihai, North China). The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 μg/L, which was 25.6% to 73.8% of total particulate organic carbon (TPOC) during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water (F =0.993, P= 0.003). Evaluation showed that for 17 000 tons (wet weight) of annual scallop production, the kelp contributed about 139.3 tons of carbon (535.8 tons of dry mass). This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.     

Antioxidant system responses in two co-occurring green-tide algae under stress conditions

Green tides have occurred every year from 2007 to 2014 in the Yellow Sea. Ulva prolifera(Müller) J. Agardh has been identified as the bloom-forming alga,co-occurring with U. intestinalis. We observed distinct strategies for both algal species during green tides. U. prolifera exhibited a high abundance initially and then decreased dramatically,while U. intestinalis persisted throughout. The antioxidant system responses of these two macroalgae were compared in the late phase of a green tide(in-situ) and after laboratory acclimation. Lipid peroxidation and antioxidant system responses differed significantly between the two. Malondialdehyde and hydrogen peroxide contents increased significantly in-situ in U. prolifera,but not in U. intestinalis. In U. prolifera,we observed a significant decrease in total antioxidant ability(T-AOC),antioxidant enzymes(SOD and Apx),and non-enzyme antioxidants(GSH and As A) in-situ. U. intestinalis showed the same pattern of T-AOC and SOD,but its Gpx,Apx,and GSH responses did not differ significantly. The results suggest that U. prolifera was more susceptible than U. intestinalis to the harsh environmental changes during the late phase of a Yellow Sea green tide. The boom and bust strategy exhibited by U. prolifera and the persistence of U. intestinalis can be explained by differences in enzyme activity and antioxidant systems.     

深海细菌太平洋火色杆菌中α-淀粉酶基因amy608的克隆表达及其酶学性质研究

从来自深海沉积物的太平洋火色杆菌(Flammeovirga pacifica)的基因组中发现了一个全长为1 875 bp的α-淀粉酶基因amy608.该基因在数据库中找不到具有同源性的序列,而所编码的Amy608蛋白与已注册蛋白的氨基酸序列相似性最高仅为56%,但具有α-淀粉酶水解活性所必需的保守基序DXEXD.进化树分析表明其属于糖苷水解酶13(GH13)家族第二亚家族.构建了p ColdΙ-amy608表达载体,在大肠杆菌中进行重组Amy608蛋白的异源表达,并采用镍离子亲和层析柱对重组蛋白进行了纯化.酶学性质分析表明,重组酶Amy608的最适作用温度为40℃,在40℃保温4 d后,仍保留70%以上的酶活,显示出良好的中温热稳定性;最适p H值为7.0,p H值范围在6~9时仍保留60%以上的酶活力,表明该酶具有较宽的p H值作用范围.Ca2+、Na+、K+对α-淀粉酶Amy608有激活作用,尤其是Ca2+可使酶活显著提高40%.薄层色谱分析结果显示该酶水解可溶性淀粉的最终产物以葡萄糖为主,表明该酶是一个糖化型淀粉水解内切酶.这些结果表明Amy608为GH13家族第二亚家族中一个新型的α-淀粉酶.     

From sequential to parallel growth of cities: Theory and evidence from Canada

This paper examines city growth patterns and the corresponding city size distribution evolution over long periods of time using a simple New Economic Geography(NEG) model and urban population data from Canada. The main findings are twofold. First, there is a transition from sequential to parallel growth of cities over long periods of time: city growth shows a sequential mode in the stage of rapid urbanization, i.e., the cities with the best development conditions will take the lead in growth, after which the cities with higher ranks will become the fastest-growing cities; in the late stage of urbanization, city growth converges according to Gibrat′s law, and exhibits a parallel growth pattern. Second, city size distribution is found to have persistent structural characteristics: the city system is self-organized into multiple discrete size groups; city growth shows club convergence characteristics, and the cities with similar development conditions eventually converge to a similar size. The results will not only enhance our understanding of urbanization process, but will also provide a timely and clear policy reference for promoting the healthy urbanization of developing countries.