Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

3种蛏类线粒体16SrRNA和COI基因片段的序列比较及其系统学初步研究

对3种蛏类大竹蛏（Solen grandis），长竹蛏（Solen strictus）和小刀蛏（Cultellus attenuatus）的线粒体16SrRNA和COI基因片段序列进行了比较并对其系统学进行了初步研究。得到的序列总长度分别为472-481bp（16S）和658bp（COI）。3种蛏序列的碱基组成均显示出较高的A＋T比例（16SrRNA基因62．1％；COI基因62．8％）。对位排序比较表明，16SrRNA片段种内个体间变异较小，3种类间存在128个碱基变异位点（其中包括127个简约信息位点）和5个插入／缺失位点，总共12个碱基长；COI片段有200个碱基存在变异，其中包括191个简约信息位点，不存在任何插入／缺失位点。数据分析结果表明16SrRNA和COI基因片段大竹蛏与长竹蛏两片段的遗传距离分别为0．0856和0．1712，两竹蛏类与小刀蛏的遗传距离分别为0．3054，0．2798和0．2662，0．2933。作者认为小刀蛏与竹蛏之间的遗传距离已达到科之间的水平，结果支持将其提升为刀蛏科的分类观点。3种蛏类线粒体16SrRNA和COI基因在种间明显的多态性，证实了16SrRNA和COI基因序列均普遍适用于蛏类种及以上阶元的系统学分析。     

Non-Simultaneous Failure of Ice in Front of Multi-Leg Structures

Because the multi-leg jacket structure is the major type of offshore structures in the Bohai Sea, the study of non-si-multaneous failure of i鏴 on multi-leg structures is important. However, the non-simultaneous failure has not been consid-ered in engineering design until now, obviously resulting in costly design and notable waste. To resolve this problem, this paper, by means of analysis of experimental data, calculates the coefficient of the non-simultaneous failure for the double-pile structure, the square four-leg structure, the single-line multi-pile structure, and the conical structure, respectively, and provides some reference criteria for engineering design.     

The Structure of A Turbulent Jet in A Crossflow-Effect of Jet-Crossflow Velocity

A comprehensive numerical study on the three-dimensional structure of a turbulent jet in crossflow is performed. The jet-to-crossflow velocity ratio (R) varies in the range of 2 - 16; both vertical jets and inclined jets without excess streamwise momentum are considered. The numerical results of the Standard two-equation k-ε model show that the turbulent structure can be broadly categorised according to the jet-to-crossflow velocity ratio. For strong to moderate jet discharges, i.e. R> 4, the jet is characterized by a longitudinal transition through a bent-over phase during which the jet becomes almost parallel with the main freestream, to a sectional vortex-pair flow with double concentration maxima; the computed flow details and scalar mixing characteristics can be described by self-similar relations beyond a dimensionless distance of around 20-60. The similarity coefficients are only weakly dependent on R. The cross-section scalar field is kidney-shaped and bifurcated, vvith distinct double concentr     

Regression Formulations for the Stress at Hot Spot of Multiplanar Tubular DT Joints

- Stress concentration analysis of multiplanar tubular DT joints plays an important role in the fatigue design of offshore platforms. A semi-analytic method for stress analysis under the condition of any loads is briefly introduced in the paper. Nineteen multiplanar tubular DT joints with one of two braces of the same dimension subjected to axial loads and out- of- plane bending moments are computed for parametric stress analysis by using the present method. The influence of geometrical parameters on the stresses of multiplanar tubular DT joints is discussed and compared with corresponding uniplanar T joints. The regression formulae for the stress at hot spot of multiplanar DT joints are found by modification of SCF of corresponding uniplanar T joints. The parametric formulae for the maximum stress by superposition. Finally, the influences of stiffening effect and load-interaction effect on the maximum stress of DT joints are discussed.     

复杂网络河的数值模拟

依据复杂网络河的特点及其内在联系，本文建立了其特征矩阵及由Newton-Raphson格式表述的隐式圣－维南差分方程组的系数矩阵，经输入边界条件及解方程组，可得到不同各支段上，下游端点上的水位和流量值。     

大型海藻龙须菜与锥状斯氏藻间的营养竞争研究

利用生物和化学方法研究了大型海藻龙须菜（Gracilaria lemaneiformis）与赤潮生物锥状斯氏藻（Scrippsiella trochoidea （Stein） Loeblich Ⅲ）共培养时二者的生长情况,以及二者之间营养盐NO-3、PO3-4竞争的情况.结果显示,两者共培养时,由于龙须菜的影响,锥状斯氏藻的生长周期以及所能达到的最大细胞密度与对照组相比都有所下降,且受抑制程度随龙须菜起始密度的增大而增强;而锥状斯氏藻对于龙须菜的生长不构成明显的影响.当龙须菜起始密度较低时（0.2、0.1gFW/L）,共培养组微藻细胞密度的下降是因为水体中NO-3的耗尽;当龙须菜起始密度较高时（0.5gFW/L）,共培养组微藻细胞密度的下降可能是因为龙须菜与微藻之间的“互荫效应”,或者龙须菜能够分泌出足够浓度的克生物质所致.龙须菜可作为有效吸收营养盐的大型海藻,用以降低近海水域富营养化程度及有害赤潮发生的几率.     

虾夷扇贝人工诱导雌核发育精子遗传失活及紫外线照射对精子形态结构影响的研究

在1698μWcm-2s^-1的紫外线照射强度下，虾夷扇贝雌核单倍体的最佳诱导时间为60s。从早期胚胎存活率可以观察到Hertwig效应的存在。随着照射时间增加，受精率明显下降，而受精卵在到达D形幼虫期之前便停止发育。扫描电镜显示，随着紫外线照射强度的增加。精子顶体和鞭毛结构趋向于受到更大程度的破坏，直到完全失去顶体和鞭毛。并指出这可能是造成受精率降低的主要原因。     

海洋细菌对赤潮藻生长及其产毒量的影响

采用麻痹性贝类毒素小白鼠生物测定法 ,研究了在可控生态条件下两株海洋细菌S1 0 、P42 对塔玛亚历山大藻生长及其产毒量的影响。结果表明 ,菌株S1 0 在较高浓度下对藻细胞的生长有明显的抑制作用 ,在较低浓度下抑藻生长作用较弱 ,不同浓度的菌株S1 0 均能有效地抑制藻细胞内麻痹性贝类毒素的产生 ,且在较低浓度下效果较好 ;菌株P42 对该藻的作用恰好与S1 0 相反 ,在较低浓度下明显抑制藻细胞的生长 ,不同浓度的菌株P42 也能有效地抑藻产毒 ,且在较高浓度下作用较明显。实验用的藻株毒力约为 ( 0 .95— 1 2 .1 4)× 1 0 - 6MU/cell,属于低毒藻株 ,该藻株在培养第 1 4天达到毒性最高峰 ,峰值为 1 2 .1 4× 1 0 - 6MU/cell,之后逐渐下降。讨论了海洋细菌在赤潮生物防治中的应用前景     

Effect of Sea Level Variation on Tidal Characteristic Values for the East China Sea

1 .IntroductionTheglobalairtemperatureroseabout 0 .5～ 0 .6°Coverthepast 2 0thcentury ,andtheglobalmeansealevelincreasedbyabout2 0cmduringtheperiod .Theregionalmeansealevelriseswiththerisingglobalmeansealevel.Zuoetal.( 1 997)indicatedthatthemeanrisingrateofabsolutemeansealevelalongtheChinacoastontheassumptionofunifiedisostaticdatumis 2mm a .Woodworth( 1 999)analyzedsealevelspanning 1 76 8tothepresentinLiverpool,andobtainedaseculartrendforheperiodupto 1 880of0 .39± 0 .1 7mm a ,andatrendfort…