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Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.     

测绘工程专业人力资源现状与毕业生需求研究

随着国民经济的快速发展,测绘行业得到了迅速发展壮大.江苏省是测绘人力资源大省,同时是测绘教育大省.通过抽样调查方法对江苏省测绘工程专业人力资源现状与毕业生需求情况进行了详细研究.研究得出江苏省测绘行业人才队伍不断壮大,未来需求量不断增加,有利于毕业生就业.     

滁州市不可移动文物管理信息系统设计和实现

以滁州市为例,在WebGIS技术支持下,通过设计和实现不可移动文物普查与管理数据库、地图基本操作、空间查询分析、文物风险评估以及文物信息输出等功能,改进文物普查与管理工作的方法和手段,提高管理工作的效率和信息化水平,为科研及文物普查管理部门提供辅助决策支持的新思路和新方法.     

Word2003文档中插入AutoCAD2004图形的三种方法

对于测绘技术人员来说,需要编写设计书、技术总结、监测方案等测绘类文档。利用Word2003自带的绘图工具,远远不能满足要求。介绍了“利用复制＋粘贴工具”、“转换图像格式”、“使用插入对象”三种方式在Word2003文档中插入AutoCAD2004图形的三种方法,     

基于模糊理论的时空数据模型及算法解决

针对当前时空数据模型中存在的主要问题,本文提出了一种基于模糊理论的时空数据模型.该模型从时空对象演化的角度出发,结合模糊隶属度的概念,力求精确表达对象演化的整个过程及对象之间的相互关系.在此基础上,进一步分析了该模型的数据结构以及算法解决,为时空数据模型的研究提供了新的思路.     

VRS RTK在地籍测量中的应用与研究

为了解决地籍测量外业要求时间短、精度较高等技术问题,在理论研究的基础上,在某市城区采用了VRS RTK技术建立测量控制网.通过成果分析得知.由于基于载波相位的GPS相对定位具有较高精度,只要控制网变形可以忽略或者进行建模,则不需要进行相邻控制点的联测,因此布设VRS RTK是非常适合的,减少了传统控制网埋石造标工作量.在RTK多测站解算中,建立了由电离层、对流层和卫星轨道引起的GPS观测误差在空间和时间的相关模型,得出在人口密集或重要经济地区布设局域GPS网的设计与测量模式.