A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.     

天津近海小型底栖动物丰度研究

2006年7月至2007年10月在渤海湾天津近海的15个站位,分春、夏、秋、冬4个航次进行了小型底栖动物丰度的调查.通过对未受扰动沉积物样品中的生物分析,共采集到线虫、桡足类、多毛类、介形类、寡毛类、双壳类、动吻类等小型底栖动物类群和少量未鉴定实体,其中线虫为优势类群,占总丰度的90%以上.调查海域小型底栖动物丰度春、夏、秋、冬依次为(405.4±154.8)ind/10cm2,(417.6±38.6)imd/10 cm2,(161.6±64.5)ind/10cm2和204.7±69.7ind/10 cm2,区内的分布以中部海域居多.小型底栖动物丰度值存在季节变化,春季和夏季平均丰度值较高,夏季略高于春季;秋冬季值偏低.小型底柄动物多分布于沉积物0～5 cm层次,占总量的85.9%～92.9%.春季和秋季的小型底栖动物丰度值与沉积物叶绿素a含量显著相关.与我国近海海域研究资料比较显示,目前渤海湾天津近海小型底栖动物丰度值略低.     

山东昌邑国家级海洋生态特别保护区生态环境现状及管护对策研究

山东昌邑国家级海洋生态特别保护区是目前国内唯一以柽柳林生态系统为主要管理和保护对象的国家级海洋保护区。为系统、全面地了解保护区内生态环境和保护物种的变化趋势,文章对山东昌邑国家级海洋生态特别保护区生态环境现状进行了监测与评价,并根据评价结果,分析了保护区保护与管理中存在的问题。研究表明,该保护区生态环境一直处于亚健康状态,为促进保护及管护,提出了加强保护区生态建设,改变保护区开发利用模式;加强能力建设,实现保护区动态监控;协调发展与保护的关系,实现资源可持续利用;大力开展柽柳生态修复,恢复柽柳的资源状况的对策建议。     

Spatio-temporal Variation of Soil Respiration and Its Driving Factors in Semi-arid Regions of North China

Soil respiration (SR) is the second-largest flux in ecosystem carbon cycling. Due to the large spatio-temporal variability of environmental factors, SR varied among different vegetation types, thereby impeding accurate estimation of CO₂ emissions via SR. However, studies on spatio-temporal variation of SR are still scarce for semi-arid regions of North China. In this study, we conducted 12-month SR measurements in six land-use types, including two secondary forests (Populus tomentosa (PT) and Robinia pseudoacacia (RP)), three artificial plantations (Armeniaca sibirica (AS), Punica granatum (PG) and Ziziphus jujuba (ZJ)) and one natural grassland (GR), to quantify spatio-temporal variation of SR and distinguish its controlling factors. Results indicated that SR exhibited distinct seasonal patterns for the six sites. Soil respiration peaked in August 2012 and bottomed in April 2013. The temporal coefficient of variation (CV) of SR for the six sites ranged from 76.98% to 94.08%, while the spatial CV of SR ranged from 20.28% to 72.97% across the 12-month measurement. Soil temperature and soil moisture were the major controlling factors of temporal variation of SR in the six sites, while spatial variation in SR was mainly caused by the differences in soil total nitrogen (STN), soil organic carbon (SOC), net photosynthesis rate, and fine root biomass. Our results show that the annual average SR and Q₁₀ (temperature sensitivity of soil respiration) values tended to decrease from secondary forests and grassland to plantations, indicating that the conversion of natural ecosystems to man-made ecosystems may reduce CO₂ emissions and SR temperature sensitivity. Due to the high spatio-temporal variation of SR in our study area, care should be taken when converting secondary forests and grassland to plantations from the point view of accurately quantifying CO₂ emissions via SR at regional scales.     

山西寿阳—阳泉地区石炭—二叠系沉积环境及其沉积特征

本区石炭—二叠系是在海湾背景下发育的一套由滨海平原—三角洲平原—冲积平原沉积组成的海退层系。主要沉积环境是三角洲平原,其上部为冲积平原包括网状河和辫状河;下部为海湾（或分支间湾）、潮下碳酸盐岩、潮间介壳滩、碎屑岩潮坪及滨海沼泽湿地等。三角洲为河控浅水三角洲。上述沉积环境,随海水退却在时空上具明显的规律性变化。     

MRI对肺癌分析的价值

本文分析了30例肺癌的 MRI 及手术病理分期的结果。结果表明:MRI 能够比较准确地显示肿块的大小、范围、部位及叶以上支气管的受累情况;容易显示胸壁、纵隔、隔肌及远处转移,对胸壁受侵犯诊断准确率为86.7%;无需使用造影剂能直接显示肺门及纵隔区大血管的受累与否、诊断准确率为83.3%;对肺门及纵隔淋巴结转移诊断准确率为90%,敏感性为91.3%,特异性为85.7%;与手术病理分期结果比较,MRI 对肺癌分期准确率为80%。故认为 MRI 是对肺癌分期癌一种准确的无创性新方法。     

建立涌水量曲线方程的最小残差法

本文在综述了目前稳定流涌水量经验方程建立方法的基础上,根据最小二乘法原理,提出了一种新的方法—最小残差法,并给出了计算实例。计算表明,最小残差法是建立稳定法涌水量经验方程的一种较好方法,计算过程在微机上易于实现。     

从灰度变化获取表面形状的研究

提出了一种基于可信度操作的从灰度变化信息获取表面形状的方法，并结合在频率域的强制可积约束，把对表面梯度空间（ｐ，ｑ）的处理经过高斯球变换到（ｆ，ｇ）空间处理，不仅较好地保证了算法的收敛性和可靠性，而且有利于边界条件的确定。     

汤原断陷断裂构造特征及其对油气成藏的控制作用

汤原断陷是典型的受断裂控制的陆相盆地。盆地内断裂极为发育。断裂的发育主要有 Ｎ Ｅ向和 Ｎ Ｗ － Ｎ Ｗ Ｗ 方向,断裂的分布具有东西分带,南北分群的特征。按断裂的成因类型可划分出７ 种类型。断裂对油气生成、运聚及成藏的控制作用主要表现在：基底大断裂控制了烃源岩的展布,主干断裂控制了圈闭的形成和分布,断裂为油气运移提供了通道作用,断裂的封闭性为油气的聚集成藏提供了遮挡条件。