印尼贯穿流源区马鲁古海与哈马黑拉海水团来源的季节和年际变化

So far, large uncertainties of the Indonesian throughflow(ITF) reside in the eastern Indonesian seas, such as the Maluku Sea and the Halmahera Sea. In this study, the water sources of the Maluku Sea and the Halmahera Sea are diagnosed at seasonal and interannual timescales and at different vertical layers, using the state-of-the-art simulations of the Ocean General Circulation Model(OGCM) for Earth Simulator(OFES). Asian monsoon leaves clear seasonal footprints on the eastern Indonesian seas. Consequently, the subsurface waters(around 24.5σ_θ and at ～150 m) in both the Maluku Sea and the Halmahera Sea stem from the South Pacific(SP) during winter monsoon, but during summer monsoon the Maluku Sea is from the North Pacific(NP), and the Halmahera Sea is a mixture of waters originating from the NP and the SP. The monsoon impact decreases with depth, so that in the Maluku Sea, the intermediate water(around 26.8σ_θ and at ～480 m) is always from the northern Banda Sea and the Halmahera Sea water is mainly from the SP in winter and the Banda Sea in summer. The deep waters(around27.2σ_θ and at ～1 040 m) in both seas are from the SP, with weak seasonal variability. At the interannual timescale,the subsurface water in the Maluku Sea originates from the NP/SP during El Ni?o/La Ni?a, while the subsurface water in the Halmahera Sea always originates from the SP. Similar to the seasonal variability, the intermediate water in Maluku Sea mainly comes from the Banda Sea and the Halmahera Sea always originates from the SP. The deep waters in both seas are from the SP. Our findings are helpful for drawing a comprehensive picture of the water properties in the Indonesian seas and will contribute to a better understanding of the ocean-atmosphere interaction over the maritime continent.     

深海细菌太平洋火色杆菌中α-淀粉酶基因amy608的克隆表达及其酶学性质研究

从来自深海沉积物的太平洋火色杆菌(Flammeovirga pacifica)的基因组中发现了一个全长为1 875 bp的α-淀粉酶基因amy608.该基因在数据库中找不到具有同源性的序列,而所编码的Amy608蛋白与已注册蛋白的氨基酸序列相似性最高仅为56%,但具有α-淀粉酶水解活性所必需的保守基序DXEXD.进化树分析表明其属于糖苷水解酶13(GH13)家族第二亚家族.构建了p ColdΙ-amy608表达载体,在大肠杆菌中进行重组Amy608蛋白的异源表达,并采用镍离子亲和层析柱对重组蛋白进行了纯化.酶学性质分析表明,重组酶Amy608的最适作用温度为40℃,在40℃保温4 d后,仍保留70%以上的酶活,显示出良好的中温热稳定性;最适p H值为7.0,p H值范围在6~9时仍保留60%以上的酶活力,表明该酶具有较宽的p H值作用范围.Ca2+、Na+、K+对α-淀粉酶Amy608有激活作用,尤其是Ca2+可使酶活显著提高40%.薄层色谱分析结果显示该酶水解可溶性淀粉的最终产物以葡萄糖为主,表明该酶是一个糖化型淀粉水解内切酶.这些结果表明Amy608为GH13家族第二亚家族中一个新型的α-淀粉酶.     

南海沉积物细菌胞外蛋白酶在家族水平上的多样性

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。     

Preface to the special issue “Tectonics and depositional infilling of western Pacific marginal sea”

Marine Geophysical Research -     

A study on one-dimensional consolidation of layered structured aquitard soils in a leakage system

Analytical solutions expressed using the hydraulic head for the one-dimensional consolidation of layered structured aquitard soils in a leakage system are deduced by transforming the n-layered structured soils into n + i (i = 1,2,…, n)-layered soils with gradually changing thicknesses, therein considering the behaviors of structured aquitard soils. The solutions are given in the case of instantaneous and linear decreasing confined aquifer water levels. By comparing three different examples, the effects of the structured behaviors on the consolidation characteristics are analyzed for the instantaneous decline of the confined aquifer water level.     

Lateral dynamic response of a partially embedded pile subjected to combined loads in saturated soil

In this article, an analytical solution is proposed to investigate the lateral dynamic response of a pile which is partially embedded in saturated soil layer and subjected to combined lateral and vertical loads. The saturated soil is described by Biot’s poroelastic theory and the resistance of soil is derived by potential function method. The governing equation of the pile is solved by coupling soil resistance and continuity conditions between the pile and the soil. The dynamic impedances of the pile are then obtained through transfer matrix method. To verify the validity of the proposed procedure, the present solution is compared with available solution for an idealized case. Finally, a parametric study is performed to investigate the effects of various parameters on the stiffness and damping properties of the pile-soil system. It is found that permeability of the soil and vertical load has significant effects on the dynamic response of the pile.     

Analytical Theory for One-Dimensional Consolidation of Soil Induced by Time-Dependent Pumping and Loading

Analytical solutions are derived for one-dimensional consolidation induced by time-dependent pumping and loading, in which both the effects of different pumping types and loading conditions are considered, i.e., step pumping, cyclic pumping, constant loading, and step loading. Based on the solutions obtained, some diagrams are prepared and the relevant consolidation behavior of soil is discussed in detail. It has been shown that the consolidation behavior is greatly influenced by pumping velocity, loading pressure, pumping and loading type.     

Geophysical investigations of crust-scale structural model of the Qiongdongnan Basin, Northern South China Sea

Rifting of the Qiongdongnan Basin was initiated in the Cenozoic above a pre-Cenozoic basement, which was overprinted by extensional tectonics and soon after the basin became part of the rifted passive continental margin of the South China Sea. We have integrated available grids of sedimentary horizons, wells, seismic reflection data, and the observed gravity field into the first crust-scale structural model of the Qiongdongnan Basin. Many characteristics of this model reflect the tectonostratigraphic history of the basin. The structure and isopach maps of the basin allow us to reconstruct the history of the basin comprising: (a) The sediments of central depression are about 10 km thicker than on the northern and southern sides; (b) The sediments in the western part of the basin are about 6 km thicker than that in the eastern part; (c) a dominant structural trend of gradually shifting depocentres from the Paleogene sequence (45–23.3 Ma) to the Neogene to Quaternary sequence (23.3 Ma–present) towards the west or southwest. The present-day configuration of the basin reveals that the Cenozoic sediments are thinner towards the east. By integrating several reflection seismic profiles, interval velocity and performing gravity modeling, we model the sub-sedimentary basement of the Qiongdongnan Basin. There are about 2–4 km thick high-velocity bodies horizontal extended for a about 40–70 km in the lower crust (v > 7.0 km/s) and most probably these are underplated to the lower stretched continental crust during the final rifting and early spreading phase. The crystalline continental crust spans from the weakly stretched domains (about 25 km thick) near the continental shelf to the extremely thinned domains (<2.8 km) in the central depression, representing the continental margin rifting process in the Qiongdongnan Basin. Our crust-scale structural model shows that the thinnest crystalline crust (<3 km) is found in the Changchang Sag located in the east of the basin, and the relatively thinner crystalline crust (<3.5 km) is in the Ledong Lingshui Sag in the west of the basin. The distribution of crustal extension factor β show that β in central depression is higher (>7.0), while that on northern and southern sides is lower (<3.0). This model can illuminate future numerical simulations, including the reconstruction of the evolutionary processes from the rifted basin to the passive margin and the evolution of the thermal field of the basin.     

南海西部海域新生代构造古地理演化及其对油气勘探的意义

南海西部海域构造复杂,主要发育有北东—北东东向、北西向和近南北向3组深大断裂。其中,北西向断裂与板块汇聚、碰撞有关,多具走滑性质;北东—北东东向断裂具有与中国东部裂谷盆地相似的发育特点,呈张扭性质;近南北向断裂可能是南海在扩张活动期间于洋、陆壳过渡部位形成的走滑调节断裂,是洋盆扩张的西部边界。新生代里,南海经历了4次成盆事件与3期扩张活动,盆地经历了古新世—中始新世陆缘断陷、渐新世—早中新世扩展与中中新世以来的热沉降3个演化阶段。陆缘断陷阶段的充填系列主要是北东—北东东向与北西向的河流—冲积扇、湖泊沼泽等陆相沉积及火山岩等;盆地扩展阶段表现为中-小型断陷、断-坳陷逐渐复合与联合为大-中型坳陷,古地理格局逐渐由河流与湖沼陆相环境演变为滨海至浅海相的沉积环境;热沉降阶段的成盆活动逐渐减弱以至停止,地层表现超覆,盆地出现联合迹象。结合以往勘探与油气资源调查成果分析,认为南海西部海域陆架陆坡区发育的大-中型沉积盆地石油地质条件良好,蕴藏着丰富的油气资源,勘探潜力巨大。     

Effect of Groundwater Fluctuations on Pore Pressures and Earth Pressures on Coastal Excavation Retaining Walls

Pore water and earth pressures acting on retaining structures are investigated using an efficient coastal double-layered excavation model to determine offshore excavation responses to groundwater fluctuations outside foundation pits. Total pore water pressure includes excess pore water pressure (due to groundwater fluctuations) and steady pore water pressure (due to steady seepage) determined using one-dimensional consolidation theory of double-layered soil and one-dimensional steady-state flow theory, respectively. Rankine's active and passive earth pressures are obtained from pore water pressure. This method is applicable to arbitrary groundwater fluctuation conditions. How physical parameters affect pore water pressure is numerically investigated using examples, demonstrating the method's practicality for calculating pore water and earth pressures.