Methods for extraction of microorganism DNA from glacier surface snow

In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard- Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K (1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from glacier surface snow.     

构建旅游中心城市之研究——以荆门市为例

在探讨旅游中心城市概念、地位及作用的基础上，以荆门市为例，分析了荆门市旅游产业发展中存在的问题、研究了荆门市建设为区域旅游中心城市的条件和优势，包括较为发达的城市经济、丰富的资源、良好的区位、科学的规划、完善的设施等，并借用中心地理论来说明荆门市应该成为区域旅游中心城市，最后列出了具体思路。     

Physicochemistry and Mineralogy of Storm Dust and Dust Sediment in Northern China

Dust sediments collected from 1995 to 1998 in Beijing, Dunhuang, Inner Mongolia, Kashi, the Kunlun Mountains, Lanzhou, Ningxia, the Taklimakan Desert, and Xi‘an, China, were characterized in terms of their physical, chemical, and mineralogical properties. Most aerosols and dust analysed ranged in texture from silty clay to clay loam. Their median particle diameters (Mds) generally ranged between 5 to 63μm,coinciding with those of loess from central China and the finest sand from northwestern China. The dust sediments were characterized by a predominance of SiO2 and Al2O3, followed by K2O. Their SiO2/Al2O3and K2O/SiO2 molar ratios ranged from 5.17 to 8.43 and from 0.009 to 0.0368, respectively. The mass concentration spectrum during a dust storm showed a single peak, rather than the triple peak generally observed under clear sky conditions. The dominant minerals were chlorite, illite, calcite, and dolomite.These physical, chemical, and mineralogical properties were consistent with those of aeolian soils and loess in western and central China. The results suggest that aerosols and fine-gained fractions of dust sediments collected in northern China are mainly composed of soil material transported from the arid and semiarid regions of China and Mongolia by prevailing winds. The rate of deposition and properties of dust falling on eastern China were strongly influenced by meteorological conditions, season, latitude, longitude, and altitude of the sampling sites.     

High-resolution satellite image segmentation using Hölder exponents

Texture in high-resolution satellite images requires substantial amendment in the conventional segmentation algorithms. A measure is proposed to compute the Hölder exponent (HE) to assess the roughness or smoothness around each pixel of the image. The localized singularity information is incorporated in computing the HE. An optimum window size is evaluated so that HE reacts to localized singularity. A two-step iterative procedure for clustering the transformed HE image is adapted to identify the range of HE, densely occupied in the kernel and to partition Hölder exponents into a cluster that matches with the range. Hölder exponent values (noise or not associated with the other cluster) are clubbed to a nearest possible cluster using the local maximum likelihood analysis.     

Simulating the moderating effect of a lake on downwind temperatures

A steady-state, two-dimensional numerical model is used to simulate air temperatures and humidity downwind of a lake at night. Thermal effects of the lake were modelled for the case of moderate and low surface winds under the cold-air advective conditions that occur following the passage of a cold front. Surface temperatures were found to be in good agreement with observations. A comparison of model results with thermal imagery indicated the model successfully predicts the downwind distance for which thermal effects due to the lake are significant.     

新疆和田河流域柽柳沙堆的生物地貌发育过程

Tamarix nabkha is one of the most widespread nabkhas,distributing in the arid region of China.Based on the observations outdoors and the simulation experiments in laboratories,analysis in this paper refers to the biological geomorphologic features and growth process of Tamarix nabkhas in the middle and lower reaches of the Hotan River,Xin- jiang.And the results indicate that the ecological type of Tamarix in the study area is a kind of Tugaic soil habitat based on the deep soil of the Populus Diversifolia f...     

基于大型科研基础设施共享的区域创新网络研究——以山东省为例

在产业革命和科技革命的背景下,创新在区域发展中发挥着越来越重要的作用,网络化创新模式成为当下创新的主流模式之一.基于创新网络视角,利用大型科研仪器共享大数据,对山东省创新网络的空间特征和主体特征进行分析,研究发现:山东省创新网络在形态上呈现出以济南为创新核心辐射带动济宁和淄博等周边边缘城市发展,同时形成东部青岛、威海和...     

基于引文分析法的“点-轴系统”理论研究述评

论文采用引文分析法,对陆大道院士所著《关于“点-轴”空间结构系统的形成机理分析》一文的349篇引文进行分析,从引文数量、引文作者、发文期刊、研究案例地、学科领域、研究主题、引用动机7个方面,探究“点-轴系统”理论的研究现状。结果表明：自2002年以来,“点-轴系统”理论的引文数量整体呈上升趋势,研究主题聚焦于区域经济和旅游发展;研究案例地以省级和市级中观尺度为主,多为单一型研究区,主要集中在西北丝绸之路经济带、东北辽宁经济圈、西南藏滇川渝、东部长三角四大热点区域;引文质量普遍较高,并且具有多学科交叉应用的特点,但批判性思考和创造性提升相对较少。基于此,论文从拓展研究和实践应用2个角度对“点-轴系统”理论未来发展进行了展望,以期为相关研究提供参考。     

白令海峡治理的合作机制研究

近年来随着北极冰盖的融化,北极通航已成为可能,而白令海峡作为北极航道重要的出口/入口,其战略地位也将逐步显现并被各国关注。在讨论白令海峡的治理与合作时,首先要明确白令海峡是用于国际航行的海峡,而作为海峡沿岸国的美国和俄罗斯对于白令海峡的管控与合作将成为影响未来北极航行的重要因素。为同时保障海峡沿岸国与使用国的利益,可借鉴马六甲海峡的治理经验,在现有治理机制的基础上进一步深化海峡沿岸国与海峡使用国的合作机制。中国作为海峡使用国也有必要积极参与白令海峡的合作治理。