不同类型建闸河口闸下淤积模拟计算

针对闸下淤积问题建立三维水动力和泥沙数学模型,在挟沙能力公式中考虑浮泥的影响,采用枚举法和最小二乘法相结合率定公式中的参数,较好地反映了建闸河口概化物理模型闸下三维水流和含沙量分布.采用该数学模型研究了短引河河道型、短引河滩槽型和长引河河道型三种类型建闸河口闸下淤积特性.结果表明,在计算条件相同的情况下,建闸河口闸下淤积速率和淤积总量与闸下河道长度、滩槽宽度有关;短引河河道型闸下初始淤积速率最大,长引河河道型闸下初始淤积速率最小;长引河河道型闸下累积淤积厚度最大,短引河滩槽型闸下累积淤积厚度最小;长引河河道型闸下河道淤积总量最大,短引河滩槽型闸下河道淤积总量最小;闸下河道两侧滩地的归槽水对闸下河道减淤作用明显.     

湛江红树林保护区现代沉积物粒度特征及其对风暴事件的响应

沉积物粒度是描述沉积环境的重要指标之一,利用沉积物粒度参数可以反演沉积物堆积过程、沉积水动力特征.通过对湛江红树林国家级自然保护内海湾、河口、潮坪区的红树林表层沉积物粒度分析的研究,结果表明：河口与海湾区的红树林沉积物以泥质为主;有堤坝保护的河口区红树林沉积物颗粒细,为悬移质体含量高且连续稳定的低能环境;红树林发育差的潮坪区红树林沉积物为砂含量较大且水动力较强的高能环境.并通过推算海湾区沉积物的沉积年代,结合样品粒度分析的结果得出了该地区风暴潮发生的大致时间.     

饲料中不同蛋白质和脂肪水平对克氏螯虾（Procambarusclarkii）生长性能、体组成和消化酶活性的影响

采用3×3因子方法,进行了饲料中不同蛋白质和脂肪水平对克氏螯虾f均重（7．03±0．05）g]生长性能、体组成和消化酶活性的影响研究。试验设3个蛋白质水平（24％、27％和30％1和3个脂肪水平（4％、7％和10％）,共9组,每组4个重复,每个重复10尾虾,为期8周。结果表明,蛋白质和脂肪水平对成活率无显著影响（P〉0...     

噻唑烷酸对肝组织GSH含量的影响及其作用机制的研究

建立丁胱亚磺酰亚胺(L-Buthionine Sulfoximine,BSO)排空小鼠肝组织谷胱甘肽(Glutathione,GSH)模型,研究噻唑烷酸(N-acetyl-glucosamine-thiazolidine-4(R)-carboxylic acid,GlcNAcCys)提高肝组织GSH含量,保护肝脏免受外源性毒物、药物损伤的可能机制。正常对照组小鼠腹腔注射生理盐水,BSO组及GlcNAcCys不同剂量组小鼠注射BSO(6mmol/kg体质量,i.p.)。2 h后,正常对照组和BSO组注射生理盐水,GlcNAcCys低、中、高剂量组分别注射不同剂量的GlcNAcCys(200、4009、00 mg/kg体质量)。6 h后,用Ellman’s法测定肝匀浆总巯基(Total Sulfhydryl,T-SH)含量;荧光分光光度法测定GSH含量;用试剂盒检测肝匀浆中谷胱甘肽还原酶(Glutathione Reductase,GR)和谷胱甘肽-S转移酶(Glutathione S-transferase,GST)活性;RT-PCR法检测谷氨酰半胱氨酸连接酶催化亚基(Glutamyl-L-cysteine Ligase,GCL)基因表达。GlcNAcCys能够提高肝匀浆中T-SH和GSH含量,增强抗氧化酶GR、GST活性,RT-PCR结果显示,GlcNAcCys能够诱导GCL mRNA的表达。GlcNAcCys能够提高肝组织T-SH、GSH的含量,增强GST、GR酶活力,增强肝脏的解毒功能,保护肝脏免受毒性中间代谢物的损伤。GlcNAcCys提高肝组织T-SH、GSH含量的机制与其诱导GCLmRNA的表达有关。     

双壳类分子生物标志物对海水重金属的响应评述

海洋双壳类动物的组织和器官对微量重金属具有高富集能力,诱导机体内的某些分子生物标志物(molecular biomarkers,MBMs)发生变化,可用于指示海水中的重金属污染水平。近10年来,许多学者在双壳类MBMs对海水重金属的响应规律方面进行了研究,其中,金属硫蛋白(MTs)、热激蛋白70(HSP70)、抗氧化防御系统等受到了较多的关注。本文在介绍这些MBMs结构和功能的基础上,重点评述了各种MBMs对海水重金属的响应特征,并提出了影响双壳类MBMs应用于海水重金属监测的因素及进一步研究的方向。     

舟山近岸海域表层沉积物中5种重金属元素的污染及潜在生态风险评价

利用2006年8月采集于舟山本岛南部近岸海域的表层沉积物监测数据,采用Hakanson生态风险指数法,分析了Hg、As、Pb、Cd和Cu等5种重金属元素含量,并进行了潜在生态风险评价,结果表明:舟山近岸海域表层沉积物中Hg、As、Pb和Cd元素的含量均符合国家海洋沉积物质量一类标准;各重金属元素均为低污染水平,潜在生态...     

日本蟳血细胞及血液生化指标的研究

以光镜和电镜观察了日本蝠（Charybdisjaponica）血细胞的形态结构,分析了各类血细胞的大小、数量比例及功能,并测定了不同发育时期及饥饿状态的日本蝽血清主要生化指标。结果表明：日本蝠血细胞可分为4种类型,从小到大依次为：无颗粒细胞、小颗粒细胞、中间型细胞和大颗粒细胞;其核质比分别为：53．23％、33．64％...     

Analysis of characteristics of time-frequency correlations between runoff, tidal range and salinity in the Changjiang Estuary

Power spectrum and cross-wavelet transform analysis was adopted to study the time-frequency characteristics and multiscale correlations between runoff, tidal range and salinity in the Changjiang Estuary based on the runoff data collected at the Datong Station, the tidal range measured at the Baozhen Station, and the salinity at the Baogang Station from 2008 to 2009. The variations of the salinity showed significant periodicity at scales of 2-3, 7-8, 14-15 and 26-30 d. The correlation between the salinity and the runoff and the tidal range were found to be significantly related to shock at scales of 5-7, 14-15, 26-30 d and 0.5 a. The correlation between the runoff and the salinity was mainly in the same phase, while the correlation between the tidal range and the salinity was in the antiphase. Different frequency bands were related to different degrees, and their relevance increased as the resonance frequency decreased. In addition, changes of the seasonal runoff were obvious. Specifically, a point of discontinuity was reached in early June with a cycle of 7-8 d, which coincided with the periodicity of plum rains in the Changjiang-Huaihe region. High-frequency changes (8-16 d period) of the salinity corresponded to the time domain in January-April 2008, February-April 2009 and October-December 2009 and exhibited an approximately 0.5 a (184 d) long frequency oscillation. Short-period changes were found to be stronger than long-period changes. Cross-wavelet transforms for the salinity, the runoff and the tidal range revealed local features in the time domain, while the significant levels of different periodic oscillations were observed in the frequency domain. The correlation characteristics of the salinity and the runoff were significant in the 80-90 d frequency domain, indicating that the major impact of the runoff on the salinity was reflected in seasonal changes. The tidal range on the small scale of 14-15 and 30-32 d was more obvious than the runoff.     

真鲷（Pagrus major）vasa基因5′端启动子克隆及表达载体构建

采用染色体步移（Genome walking）的方法克隆了真鲷vasa基因5′侧翼序列,采用生物信息学方法分析潜在的顺式作用元件,并与斑马鱼核心启动子进行比对,在此基础上构建了绿色荧光蛋白表达载体。序列分析结果显示：克隆得到的真鲷vasa基因5′侧翼序列序列长度为2762bp,其中包括TATA-box、CAAT-box...     

大菱鲆（Scophthalmus maximus）胚胎发育的形态学和组织学研究

采用显微镜和连续切片技术,观察了大菱鲆胚胎发育阶段的形态学和组织学特征。结果表明,大菱鲆胚胎发育主要经历6个时期;在14℃,胚体经108h即可孵化出膜。胚胎在64细胞期出现纬裂,分裂球分化为外层的包被层和内部的深层细胞;多细胞期形成卵黄合胞体层;低囊胚期形成囊胚腔。受精后26h30min胚盾出现。胚盘下包65%时,头突...