APPLICATION OF GEOGRAPHICAL PARAMETER DATABASE TO ESTABLISHMENT OF UNIT POPULATION DATABASE

Now GIS is turning into a good tool in handling geographical, economical, and population data, so we can obtain more and more information from these data. On the other hand, in some cases, for a calamity, such as hurricane, earthquake, flood, drought etc., or a decision-making, such as setting up a broadcasting transmitter, building a chemical plant etc., we have to evaluate the total population in the region influenced by a calamity or a project. In this paper, a method is put forward to evaluate the population in such special region. Through exploring the correlation of geographical parameters and the distribution of people in the same region by means of quantitative analysis and qualitative analysis, unit population database (1km× 1km) is established. In this way, estimating the number of people in a special region is capable by adding up the population in every grid involved in this region boundary. The geographical parameters are obtained from topographic database and DEM database on the scale of     

GPS揭示的现今地壳运动与地震前兆特征

利用“中国地壳运动观测网络”产出的GPS观测资料,采用球坐标系下非连续变形算法对资源进行计算,以此为基础,对我国大陆地块及其边界带运动与形变进行了分析。对GPS基准站的连续观测序列采用小波变换进行分解变换,分析了部分震例,探讨了从GPS连续观测序列提取地震前兆的方法。初步取得两点认识：（1）7级以上的大地震主要发生在走滑运动量大的活动边界,且与高应变率地块有关,这可以作为地震中长期预测判据;（2）GPS基准站连续观测序列中的低频段,地震前6个月或稍长时段有一定的前兆异常出现,这可以作为地震中短期预测判据。     

福建省地壳形变观测网络建设

介绍了福建省地壳形变观测网络的建设。福建省地壳形变观测网络，以GPS观测技术为主，结合精密水准、精密重力和流动地磁测量，将3者重合到GPS观测基准站上获取观测信息，初步构成一个时空相结合，点线面与长中短兼顾的高精度、高时空分辨率的地壳形变观测网络。该网络可动态监测福建省东南沿海地区地壳运动变化，为地震预测预报提供背景信息，将取得显著的社会效益和经济效益。     

大姚6级双震前的地震前兆特征

在连续形变观测资料中除了存在于整个时间域的短周期潮汐信息(固体潮)外，还有出现在某个时间段的长周期非潮汐信息．如何从时间或空间上认识和提取这些信息．成为认识地震过程和进行地震预报关键和迫切的问题之一。利用小波分析的时一频特性和奇异性检测特性，对大姚6级双震前的连续形变观测资料进行了处理，提取潮汐资料中的非潮汐信息。发现在地震前震中附近的形变台站都接收到了相同频段的异常信号．周期为几天到十几天，出现在各个台站的时间也大致相同。这些在震前具有一定时、空分布的信息有可能是地震前兆。     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

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Combined effects of light intensity and NH^＋4-enrichment on growth,pigmentation, and photosynthetic performance of Ulva prolifera （Chlorophyta）

The aim of this study was to investigate the effects of light intensity and enhanced nitrogen supply on the growth and photosynthesis of the green-tide macroalga, Ulva prolifera. Thalli of U. prolifera were grown in natural or NH 4 +-enriched seawater under two different light intensities for 7 days, and then the growth rate, pigmentation, and photosynthetic performance of the thalli were evaluated. The results show that the relative growth rate(RGR) was markedly higher under the high light level than under the low light level. Enrichment with NH 4 + enhanced the RGR under high light intensity, but did not affect RGR under low light intensity. In low light conditions, NH 4 +-enrichment resulted in a marked decrease in the maximal photosynthetic rate( P m) and the maximum carbon fixation rate( V max), but it did not affect the half saturation constant for carbon( K 0.5) or the ratio of V max to K 0.5, which reflects the carbon acquisition efficiency. In high light conditions, P m, K 0.5, and the dark respiration rate( R d) increased under NH 4 + enrichment, but V max and the V max / K 0.5 ratio decreased. Regardless of the light intensity, NH 4 +-enrichment did not affect the apparent photosynthetic efficiency( α), which refl ects the ability of the alga to use light energy at low light levels. Under both low and high light intensities, the chlorophyll a(Chl a), chlorophyll b(Chl b), and carotenoids(Car) contents in thalli were higher in NH 4 +-enriched than in natural seawater, except that there was a decrease in the Chl b content of thalli in NH 4 +-enriched seawater under low light intensity. Therefore, NH 4 + enrichment improved the growth and photosynthetic performance of U. prolifera under high light intensity, but not under low light intensity. We discuss the possible mechanisms underlying these physiological responses.     

Phylogenetic analysis of epibacterial communities on the surfaces of four red macroalgae

Macroalgal surfaces are prone to being attached by bacteria. Epibacterial community structures on marine macroalgae are host-specific but temporally and spatially variable. In this study, we investigated the structure of epibacterial communities on the surfaces of four red macroalgae, Gracilaria lemaneiformis, Gloiopeltis furcata, Mazzaella sp. and Porphyra yezoensis, by analyzing the sequences of 16 S r RNA gene libraries. Healthy individuals of all macroalgae species were collected in winter from a farm at Dalian, China. The results showed that the epibacterial communities were mainly dominated by α-Proteobacteria, γ-Proteobacteria and Bacteroidetes. Deinococcus-Thermus, Spirochaetes and ε-Proteobacteria were also found. The majority of cloned sequences shared the greatest similarity to those of culturable organisms. A large portion of sequences from the α-Proteobacteria homed in Roseobacter clade, i.e., genera Ahrensia, Roseovarius, Litoreibacter, Octadecabacter, Thaiassobacter and Sulfitobacter, while members of Bacteroidetes mainly belonged to family Flavobacteriaceae. The cloned sequences could be separated into 66 OTUs at 0.01 distance value, and rare common OTUs were found among libraries. At genus level, Pseudoalteromonas dominated Gr. lemaneiformis and Gl. furcata libraries, accounting for 72.2% and 47.3%, respectively. Sulfitobacter dominated P. yezoensis library, accounting for 35.4%. A previously undefined cluster within Deinococcus-Thermus dominated Mazzaella sp. library, accounting for 24.6% of the all. These results indicated that a broad range of bacteria inhabited the surfaces of these macroalgae.     

NUMERICAL SIMULATION OF INTERANNUAL AND INTERDECADAL VARIABILITY OF SURFACE WIND OVER THE TROPICAL PACIFIC

A global atmospheric general circulation model (L9R15 AGCMs) forced by COADS SST was integrated from 1945 to 1993. Interannual and interdecadal variability of the simulated surface wind over the tropical Pacific was analyzed and shown to agree vey well with observation. Simulation of surface wind over the central-western equatorial Pacific was more successful than that over the eastern Pacific. Zonal propagating feature of interannual variability of the tropical Pacific wind anomalies and its decadal difference were also simulated successfully. The close agreement between simulation and observation on the existence of obvious interdecadal variability of tropical Pacific surface wind attested to the high simulation capability of AGCM.