日本囊对虾Marsupenaeus japonicus)组织蛋白酶D基因克隆及表达分析

组织蛋白酶D是溶酶体天冬氨酸蛋白酶家族的主要成员,广泛参与动物机体细胞内蛋白质的降解过程,对维持细胞稳态和正常代谢具有重要的作用。为研究组织蛋白酶D在甲壳动物非特异性免疫和幼体发育过程中的作用,本研究采用RACE技术首次克隆得到日本囊对虾组织蛋白酶D基因cDNA序列,命名为MjCatD,其中开放阅读框长为1161 bp,编码386个氨基酸残基。序列分析和同源建模显示该基因编码的蛋白含有保守的N-糖基化位点、天冬氨酸蛋白酶签名序列、酶活化位点和非消化性组织蛋白酶D的特征序列,并且呈保守的双叶形结构。同源性比较和系统进化分析发现,MjCatD与斑节对虾、美洲螯龙虾和脊尾白虾相似性较高,并且与它们紧密聚为一支。实时荧光定量PCR结果显示,MjCatD基因在日本囊对虾多个组织中均有表达,其中肝胰腺中表达量最高。在白斑综合征病毒(white spot syndrome virus, WSSV)感染后3～24 h日本囊对虾肝胰腺中MjCatD的表达量逐渐下降,而在48 h急剧上调至最高表达量并且与对照组差异极显著(P<0.01)。此外,MjCatD基因在幼体发育不同阶段中也表现出明显的变化趋势。以上研究表明,MjCatD基因可能参与日本囊对虾先天免疫反应和幼体发育过程。     

西北太平洋浪流相互作用对有效波高的影响研究

西北太平洋强流区会对海浪的特征和分布产生显著的影响,尤其是研究台风过程中海流与海浪的相互作用具有重要的研究意义。本文以ROMS海洋模式和SWAN海浪模式为基础,构建了浪流耦合模式系统,对2013年10月6-17日间的台风“丹娜丝”、“百合”、“韦帕”过程中西北太平洋浪流相互作用中海流对有效波高的影响进行了研究。通过对比模式模拟有效波高与浮标观测资料,发现耦合后的有效波高比非耦合结果更接近观测值,耦合模式中海流的存在对有效波高的分布有明显的影响。研究表明,特别是在有效波高峰值处,海流引起的有效波高增大最大可达1 m。海浪浪向及流向的空间分布以及中国近海浮标处浪向与流向的时间序列表明,流向与浪向反向时,海流的影响造成有效波高增大;二者同向时,有效波高减小。海流对有效波高的调整会沿着海浪传播的方向传播相当一段距离。在西北太平洋的海浪场计算中,引入海流的耦合模式计算结果对改善强流区海浪预报具有重要意义,并且海流的模拟精度对于高精度的海浪预报非常重要。     

秋季南黄海浮游动物分布及其影响因素

基于2007年秋季在南黄海（32°20'~37°00'N;124°E以西）进行的浮游动物及环境因子大面调查;分析了秋季南黄海浮游动物种类组成、分布特征及其影响因素;主要结果如下:共鉴定浮游动物113种（不包括25种浮游幼体）;中华哲水蚤（Calanus sinicus）、强壮滨箭虫（Aidanosagitta crassa）、磷虾幼体（Euphausia larvae）和小齿海樽（Doliolum denticulatum）是秋季优势种;浮游动物丰度为（156.37±12.04）ind/m3;生物量为（172.57±10.41）mg/m3;与历史调查数据相比;本航次浮游动物丰度和生物量相对处于较高水平;磷虾幼体分布趋势与中华假磷虾（Psudeuphausia sinica）一致;说明秋季是中华假磷虾种群的一个重要的补充时期;小齿海樽在南黄海的大量出现系自身种群补充的结果;精致真刺水蚤（Euchaeta concinna）和肥胖软箭虫（Flaccisagitta enflata）主要分布在深水区;在近岸海区很少出现。中华哲水蚤、强壮滨箭虫丰度高值区倾向分布于海洋锋附近;进一步佐证了海洋锋对浮游动物的积聚作用。     

基于浮标数据的卫星雷达高度计海浪波高数据评价与校正

卫星雷达高度计是海浪有效波高（significant wave height,SWH）观测的重要手段之一,本文利用时空匹配方法对T/P、Jason-1、Envisat、Jason-2、Cryosat-2和HY-2A共6颗卫星雷达高度计SWH数据与NDBC（National Data Buoy Center,NDBC）浮标SWH数据进行对比验证,并对雷达高度计SWH数据进行校正。全部卫星雷达高度计SWH数据时间跨度为1992年9月25日到2015年9月1日,对比验证NDBC浮标共53个,包括7个大洋浮标。精度评价发现除T/P外,各卫星雷达高度计SWH的RMSE都在0.4~0.5 m之间,经过校正后,RMSE都有显著下降,下降程度最大为13.82%;对于大洋浮标,评价结果RMSE在0.20~0.28 m之间,结果明显优于全部NDBC浮标的精度评价结果;HY-2A卫星雷达高度计SWH在经过校正后数据质量与国外其他5颗卫星雷达高度计SWH数据质量差异较小。     

南海周边相对海平面变化特征及2004年苏门答腊地震影响分析

利用南海周边1989-2014年的潮汐资料和GPS长期观测资料,分析了南海周边相对海平面变化特征,以及2004年苏门答腊地震对该区域相对海平面变化的影响。研究结果表明,南海周边的相对海平面变化以上升为主,平均上升速率（4.53±0.20） mm/a,高于全球平均速率,且2004年后上升趋势加剧;南海周边相对海平面呈现6类较典型的变化特征,并存在与板块构造相对应的分区聚集现象,形成了中国东南和越南沿海、马来半岛、加里曼丹岛北部、菲律宾群岛等4个变化特征区。受2004年苏门答腊大地震的影响,马来半岛、南沙和西沙海域的地壳形变由上升趋势转为下沉,加剧了相对海平面的上升;中国东南沿海和菲律宾群岛受地震影响较小;越南沿海和加里曼丹岛北部区域的地震影响还有待进一步研究。     

琼东南盆地深水区新近系海底扇沉积特征与资源潜力

综合利用钻井、岩心、薄片及分析化验资料研究了琼东南盆地深水区新近系海底扇沉积特征,并利用最新的三维地震资料,通过井震精细标定、多属性融合技术、方差体切片、三维地貌砂体镂空等综合技术手段,精细刻画了海底扇砂体的空间分布特征。研究结果表明,深水区新近系海底扇是由陆架区的砂体滑塌并二次搬运形成,形成过程具有多期次性。受不同物源的影响,海底扇岩性和物性存在较大的差异。海底扇岩性及沉积构造具有砂质滑塌、碎屑流、浊流和深水底流改造的特征。海底扇的沉积微相、厚度、砂泥比和砂泥岩空间配置关系直接控制了地震振幅反射强度和频率的变化。砂体纵向叠置,横向连片,并被后期泥质水道切割分块形成多个岩性圈闭。综合分析认为,深水区海底扇砂体发育区烃源条件优越,储盖配置关系和圈闭条件良好,具备形成大中型岩性油气藏的有利条件,具有较大的油气勘探潜力。     

Radar-based collision avoidance for unmanned surface vehicles

Unmanned surface vehicles (USVs) have become a focus of research because of their extensive applications. To ensure safety and reliability and to perform complex tasks autonomously, USVs are required to possess accurate perception of the environment and effective collision avoidance capabilities. To achieve these, investigation into realtime marine radar target detection and autonomous collision avoidance technologies is required, aiming at solving the problems of noise jamming, uneven brightness, target loss, and blind areas in marine radar images. These technologies should also satisfy the requirements of real-time and reliability related to high navigation speeds of USVs. Therefore, this study developed an embedded collision avoidance system based on the marine radar, investigated a highly real-time target detection method which contains adaptive smoothing algorithm and robust segmentation algorithm, developed a stable and reliable dynamic local environment model to ensure the safety of USV navigation, and constructed a collision avoidance algorithm based on velocity obstacle (V-obstacle) which adjusts the USV’s heading and speed in real-time. Sea trials results in multi-obstacle avoidance firstly demonstrate the effectiveness and efficiency of the proposed avoidance system, and then verify its great adaptability and relative stability when a USV sailing in a real and complex marine environment. The obtained results will improve the intelligent level of USV and guarantee the safety of USV independent sailing.     

Numerical Analysis of Gravity Coring Using Coupled Eulerian-Lagrangian Method and a New Corer

The shortcomings of gravity corers in sampling marine sediments have been observed extensively in various field tests. In order to optimize the coring, this article provides an alternative numerical way to model the gravity coring and analyze the sampling effect. Based on this analysis, a new hydraulic hammer corer is devised. A coupled Eulerian-Lagrangian method with capability of simulating the problem involving extreme deformation, penetration is used to simulate the coring process. The results show that the hydrostatic pressure and deviator stress increase and reach their peak when the pile tip is slightly above or at the level of the observation point and then drop rapidly when the pile tip slides below the observation point. In addition, the stress path indicates that the soil element sustains plastic compression before yielding and then expands until recovering to the original state. The obvious “under-sampling” phenomenon is also well-captured by the finite element model.     

Effects of Mudstone Particle Content on Shear Strength of a Crushed Sandstone–Mudstone Particle Mixture

The present study focuses on the shear strength, deformation, and particle crushing of sand which is mixed by crushed sandstone and mudstone particles. Two triaxial tests—one for unsaturated specimens and the other for saturated specimens—were performed, along with sieve analysis tests. Results obtained from the triaxial tests indicate that, with the increment of the mudstone particle content by weight, the angle of internal friction decreases, the cohesion increases and then decreases, and both the initial angle of shearing resistance at the atmospheric pressure and decremental angle increase and then decrease. Compared to the unsaturated specimens, the deviator stress or normalized deviator stress of the saturated specimens at the same axial stress may decrease due to the wetting action. Thus, the shear strength of the tested materials may be reduced by the wetting action. Results of sieve analysis tests indicate that the mixing of some mudstone particles into the sandstone particle mixture may reduce the amount of particle crushing, but the wetting action may increase the amount. The amount of the crushed particle may arrive at a minimum of 40% of mudstone particle content by weight.     

Study on Longitudinal Deformation Profile of Rock Mass in a Subsea Tunnel

During tunnel excavation, the deformation of surrounding rock due to the unloading of rock mass will vary with time. However, the measured displacement of surrounding rock is only a part of the actual longitudinal deformation profile. There is a need to analyze the longitudinal deformation profile to identify the deformation state and evaluate the stability of surrounding rock mass. In the present article, the variation of pre-deformation of surrounding rock due to excavation was analyzed, and the release coefficient was obtained from the measured results. For the Qingdao subsea tunnel, the measured crown settlement of surrounding rock was analyzed using the regression analysis method, and the longitudinal deformation profile of rock mass was simulated using the numerical calculation method. Moreover, based on the conditions of the subsea tunnel, a solid-fluid coupling model test was carried out to check the reliability of the numerical calculation results. The results of the model test were consistent with the numerical calculation results.