Generation of reactive oxygen species in different fractions of the coelomocytes of holothurian Eupentacta fraudatrix in response to the thermostable toxin of Yersinia pseudotuberculosis in vitro

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).     

地倾斜台站数字化、模拟观测数据频率特性研究

分析了四川、云南的部分地倾斜数字化观测与模拟观测资料的特性，从频率范围，周期10天以上的FFT谱特性，固体潮波振幅，相位特征值等方面，论述了地倾斜数字化资料较模拟记录资料的信息组成变化，并对资料的分析与应用进行了初步探讨。     

Strong Tides in 1964 and 1982

1StrongtideandastronomicalconditionsPartial solar eclipse occurred 4 times in 1964, 1982 and 2000 respectively. Time interval is about 3 Saros periods (one Saros period is 18 years and 10.33～11.33 days). Total lunar eclipse occurred 2 times in 1964 and 2000 respectively and 3 times in 1982. However, there was no lunar eclipse in 1966, 1984 and 2002. It seems that they had similar astro-nomical conditions and the best was in 1982. The studies about the effect of tide on the global climate…     

Theory of stability, and regulation and control of ecological system in oasis

Starting with analysis on the evolving course of oasis and the characteristics and evolution of transitional zone between oasis and desert, in consideration of ecological elements including plant stomata resistance, area covered by vegetation, and physical elements including albedo of vegetation and bare soil, atmosphere temperature, and humidity, under the condition of the balance among net radiation flux, latent heat flux, and sensible heat flux, the following are calculated: temperatures of vegetation and bare soil in different conditions, as well as the evapotranspiration rate of ecosystem. Analysis on evapotranspiration rate indicates that it depends on both the climate of environment and the physiological and ecological conditions of plants. On certain conditions, the evapotranspiration rate of transitional zone between oasis and desert (i.e. area covered by vegetation less than 20%), in some parameter domains, appears in bifurcation or multiequilibrium state. Meanwhile, in such area, ecosystem is extremely unstable. Any minor change to the balance will cause either increase or reduction of area covered by vegetation in ecosystem, on the basis of discussion on the emergency of these phenomena. This paper is attempting to propose an effective way of destruction and rebuilt ecosystem in transitional zone. The way is to control the evaporation of plant through selecting anti-drought country plant with big stomata resistance, and modify the roughness of the underlying surface in ecosystem by establishing rational interspace structure of plant community, so as to put the degenerative ecosystem into the natural succession track. This primary theory is being verified through observation and analysis on historical data.     

用PCI中断实现射电望远镜跟踪的方法

讨论运用PCI 9054(美国PLX公司生产的接口芯片)作为接口芯片的PCI(Peripheral Component Interconnect)板卡的软硬件设计,以实现天线跟踪的两个时间同步中断。利用标准秒信号中断作为系统时钟同步信号,并同步产生时间间隔为20ms(或40、50ms,可选)的中断信号,来处理天线跟踪指令输出。中断信号通过PCI中断口INTA#接入计算机,在驱动中识别不同的中断信号,并在应用程序响应中断处理后,命令ACU(Antenna Control Unit)机,实现射电天文望远镜的同步跟踪。其控制过程分3部分阐述:硬件设计、驱动程序设计、安装及应用;着重讨论了前两者的设计方法及思路。     

Modelling the April 15 spectrum of Supernova 1993 J

We present the modeling of the ultraviolet and optical spectra obtained simultaneously on 1993 April 15 with the HST and at Lick Observatory. A Monte Carlo code is employed in the modeling and a comparison is made between models reported by different groups. With an atmosphere similar to the Sun in chemical composition, the observed spectral lines are well reproduced by a power law density structure of index around 20 except the strong H and HeI λ5876 lines which have peculiar absorption profiles. The photospheric velocity is found to be 9500 km/s and the blackbody temperature of the spectrum is 7990 K. For H and HeI λ5876, we suggest a two-component density structure which has a smoother layer located immediately outside the steeply decreasing inner envelope. The power law indices are most probably 20 and 3, respectively, with the transition point at about 13 000 km/s. In addition, this outer smooth layer serves to flatten the far UV spectrum as observed.     

Isolation and pharmacological activities of bromophenols from Rhodomela confervoides

Four bromophenols were isolated from the extract of marine red alga,Rhodomela confervoides by column chromatography and HPLC methods. By means of spectroscopic methods inclding IR, MS, 1D, and 2D-NMR techniques, their structures were elucidated as (1) 3-bromo-4,5-dihydroxy benzoic acid methyl ester; (2) bis (2,3-dibromo-4,5-dihydroxybenzyl) ether; (3) 3,4-dibromo-5-(methoxymethyl)-1,2-benzenediol and (4) 3-bromo-4,5-dihydroxy-benzaldehyde. Compound 1 was first isolated from the algae in nature, and 1, 4 were found to have selective cytotoxic activities against KB, Bel 7402 and A549 cells, 2 showed powerful antibacterial activities againstStaphylococcus aurens andPseudomonas aeruginosa. Supported by National “863” Program (No. 2004AA625030, 2001AA620503), Quingdao marine sciences project (No. 04-2-NN-26) and Key Knowledge Innovative Project of the Chinese Academy of Sciences (KZCX3-SW-215).     

用遗传算法解算病态方程

对应用遗传算法解决病态方程问题进行了探讨。利用拟合法而不是通过法方程求解参数，从而避免了法方程系数求逆，使病态方程的解答有了较好的结果。通过模拟计算并和其他方法进行比较，证明该方法是可行的和有效的。     

澳门附近海域沉积物污染状况及特征分析

掌握澳门附近海域沉积物的污染状况及特征,对于合理安排澳门海洋海岸工程疏浚泥的海洋倾倒,防止造成海洋污染有十分重要的意义。根据1997—1999年连续三年对澳门附近主要区域的海洋沉积物采样测定结果,通过分析认为澳门附近海域沉积物污染具有下列特征:1)沉积物中重金属和油类含量普遍较高,除了总Hg外均高于珠江口海区的其他港口;2)沉积物中污染物的分布受人类活动的影响明显,澳门内港受船只排污、码头作业、陆地工业和生活污水排放的影响,是澳门附近海域沉积物污染最为严重区域;3)受水质交换的影响明显,澳门外港、路环岛东北等区域受珠江径流影响水质交换较快,沉积物中污染物的含量较低,而内港由于水质交换较差,沉积物中污染物的含量相对较高;4)与沉积物的成分组成(吸附)有关,大部分污染物与沉积物中粉砂有较好的相关性,表明粉砂对污染物质有较广泛性的吸附能力,尤其是Cu、Zn、Cd、油类、有机质。