大洋硅藻席沉积与冰期旋回中的碳-氮循环

成席硅藻勃发与沉积埋藏过程链接了海洋有机碳生成-输出-埋藏以及大洋深部溶解无机碳的生成与储存的全过程,该过程及其对大洋碳-氮循环的潜在重大影响正引起学术界的关注。通过搜集整理一系列文献及数据,对成席硅藻在细胞层面的特殊功能、勃发机制以及硅藻席沉积物的时空分布特征进行了总结,尝试讨论硅藻席沉积对轨道时间尺度上全球碳-氮循环的潜在影响。由于成席硅藻具有一系列特点,包括在弱光条件下生存、利用大液泡来存储营养盐和调控浮力、与固氮蓝细菌共生、尿素循环等,导致成席硅藻在寡营养的层化水体中或者大洋锋面处容易获得生存竞争优势并发生勃发。根据沉积记录,在第四纪冰期,热带-亚热带大西洋以及热带西太平洋-东印度洋出现大规模的Ethmodiscus rex硅藻席勃发并向海洋内部输出大量有机碳。有机碳中的绝大部分都在水柱中发生降解,只有少部分保存到海底沉积物中。推测硅藻席勃发贡献了冰期深海内部“呼吸碳库”的增长以及大气二氧化碳浓度的降低。由于E. rex勃发加快了海洋上层营养盐的周转速率,因此也可能促进了冰期海洋氮储库的扩张。此外,氧同位素14/12期和4/2期间同时出现大洋无机碳碳同位素重值事件和热带-亚热带硅藻席勃发事件,二者之间可能存在机制上的关联。因此,成席硅藻是耦合大洋碳-氮循环的重要组成部分,进一步厘清地质历史时期硅藻席勃发规模及其在海洋元素循环中的作用,有助于解开冰期旋回尺度上的全球碳-氮循环之谜。     

两种虾、贝、藻综合养殖模式的初步比较

利用太平洋牡蛎(Crassostrea gigas)和孔石莼(Ulva pertusavar)与凡纳滨对虾(Litopenaeus vannamei)以2种方式搭配放养,旨在查明其各自的生态特征及养殖效果。实验中对虾设2个放养密度,分别为17尾/m2和34尾/m2;牡蛎、石莼各设1个放养密度,分别为1 700 g/m2,140 g/m2。实验结果表明,经过50 d的实验,2个密度下对虾的生长均是混养明显快于分养模式(高密度混养与分养对虾体重分别为(7.04±0.19)g,(5.20±0.22)g;低密度混养与分养对虾体重分别为(7.89±0.34)g,(5.66±0.30)g)。总产量也是混养模式稍高于分养模式[高密度混养与分养对虾产量分别为(330.92±8.91)g,(189.47±8.05)g;低密度混养与分养对虾产量分别为(264.97±11.46)g,(135.82±7.27)g]。混养模式水体中的浮游植物的含量高于分室循环水模式中的含量,其它水化学指标间没有明显差异。分养循环水模式下虾室中水体过清、透明度过高,从而导致水中光照强度和光色的变化可能是其中对虾生长较慢的主要原因。     

音响驯化技术在鲤养殖中的实验研究

探讨淡水养殖尤其是大水域网箱养鱼中由于投饵方式的不当和投饵量过多导致过剩饵料沉积引起水域环境污染的解决方法。本研究利用鱼类的听觉生物学特性,参考音响驯化技术的有关参数,结合投饵用400 Hz正弦波连续音对鲤(Cyprinus carpioLinnaeus)进行了音响驯化实验。结果表明,最初放声时,实验鱼产生惊愕反应,迅速离声源而去;但驯化6 d后,实验鱼在放声后迅速游向声源,摄食时间由最初的150 s缩短到实验后期的60 s。整个实验阶段放声组与对照组摄食时间差异极显著(P<0.01),摄食率和特定生长率无显著差异(P>0.05)。根据实验鱼的生长参数,求得了参考投饵量曲线和方程。本实验结果为音响驯化技术在淡水鱼养殖中的推广应用提供了基础数据。     

利用三维可视化平台快速计算地质灾害区塌方量的方法研究

基于三维可视化平台,利用离散点内插的方法构建地质灾害区表面TIN模型,并利用C#语言实现地质灾害区塌方量的算法.在理论上该方法所描述的地面模型逼真且能保持原始数据的原有精度,而且采用TIN进行体积计算,易于计算机实现.通过塌方区实例数据验证该方法具有速度快、精度高、作业效率好等优点,可以满足地质灾害应急需求.     

海绵共栖细菌抗菌活性及其群体感应信号的研究

利用平板涂布法从南海海绵生物样品中分离海绵共栖细菌,以枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌及酿酒酵母作为实验用指示菌对海绵共栖细菌进行了抗菌活性筛选。利用气相质谱联用仪(GC-MS)建立了群体感应信号分子酰基高丝氨酸内酯类化合物(AHLs)的检测方法,分析了活性菌株粗提液中信号分子的产生及种类。同时采用16S rDNA同源性和系统发育分析对活性菌株进行种属鉴定和系统发生学研究。结果表明,在分离到的94株海绵共栖细菌中18株(占19.1%)细菌具有抗菌活性。通过GC-MS检测确定大多数活性菌株都含有AHLs,因此提出了海绵共栖细菌抗菌活性与群体感应之间可能存在必然联系的推测。通过细菌分类鉴定,结果显示具有抗菌活性的细菌大部分属于芽孢杆菌属(Bacillus)。     

Experimental,Numerical and Simplified Theoretical Model Study for Internal Solitary Wave Load on FPSO with Emphasis on Scale Effect

Scale effect of ISWs loads on Floating Production Storage and Offloading(FPSO) is studied in this paper. The application conditions of KdV, eKdV and MCC ISWs theories are used in the numerical method. The depthaveraged velocities induced by ISWs are used for the velocity-inlet boundary. Three scale ratio numerical models λ=1, 20 and 300 were selected, which the scale ratio is the size ratio of numerical models to the experimental model.The comparisons between the numerical and former experimental results are performed to verify the feasibility of numerical method. The comparisons between the numerical and simplified theoretical results are performed to discuss the applicability of the simplified theoretical model summarized from the load experiments. Firstly, the numerical results of λ=1 numerical model showed a good agreement with former experimental and simplified theoretical results. It is feasible to simulate the ISWs loads on FPSO by the numerical method. Secondly, the comparisons between the results of three scale ratio numerical models and experimental results indicated that the scale ratios have more significant influence on the experimental horizontal forces than the vertical forces. The scale effect of horizontal forces mainly results from the different viscosity effects associated with the model’s dimension.Finally, through the comparisons between the numerical and simplified theoretical results for three scale ratio models, the simplified theoretical model of the pressure difference and friction forces exerted by ISWs on FPSO is applied for large-scale or full-scale FPSO.     

浙江6个列岛大型底栖藻类分类多样性

本文基于对七星列岛大型底栖藻类的实地调查,结合浙南3个列岛(七星列岛、南麂列岛和洞头列岛)和浙北3个列岛(渔山列岛、中街山列岛和马鞍列岛)大型底栖藻类物种组成的有关文献,构建大型底栖藻类基础数据库。采用平均分类差异指数△~+、分类差异变异指数∧~+、G-F多样性测度指数和相似性系数开展分类多样性研究。结果显示:6个列岛藻类在目级和科级的相似性很高,其值均在0.800以上;洞头列岛的△~+值最高(77.42),中街山列岛最低(72.65),这表明洞头列岛大型底栖藻类类群间的亲缘关系较远,中街山列岛藻类类群间的亲缘关系较近;七星列岛的∧~+值最高,达942.00,南麂列岛最低,仅865.12,这表明南麂列岛大型底栖藻类在不同分类阶元分布最均匀,七星列岛的藻类分布较集中;南麂列岛无论是F指数、G指数还是G-F指数的值均比其他5个列岛高,依次为18.62,4.40和0.76,这说明南麂列岛藻类的生态位分化程度较高。本研究构建了6个列岛大型底栖藻类资源与分类多样性基础数据库,为有效保护和管理大型底栖藻类提供了理论依据。     

基于微卫星(SSR)分子标记的中国对虾渤海湾增殖亲虾群体及回捕群体的遗传多样性分析

Eight microsatellite markers were used to analyze genetic diversity, level of inbreeding, and effective population size of spawner and recaptured populations of Chinese shrimp(Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay in 2013. A total of 254 and 238 alleles were identified in the spawner and recaptured populations, respectively, and the numbers of alleles(N_a) were 8–63 and 6–60, respectively. The numbers of effective alleles(N_e) were 2.52–21.60 and 2.67–20.72, respectively. The polymorphism information content ranged from 0.529 to 0.952. The observed heterozygosity(H_o) values(0.638–0.910 and 0.712–0.927) were lower than the expected heterozygosity(H_e) values(0.603–0.954 and 0.625–0.952), which indicated that the two populations possessed a rich genetic diversity. In 16 tests(2 populations×8 loci), 13 tests deviated from the HardyWeinberg equilibrium. F_(is) values were positive at seven loci and the inbreeding coefficients(F) of the two populations estimated by trio ML were 13.234% and 11.603%, suggesting that there was a relatively high degree of inbreeding. A certain level of inbreeding depression had occurred in the Chinese shrimp population. F_(st) values ranged from 0 to 0.059, with a mean of 0.028, displaying a low level of genetic differentiation in the two populations. Effective population sizes(3 060.2 and 3 842.8) were higher than the minimum number suggested for retaining the evolutionary potential to adapt to new environmental conditions. For enhancement activity in 2014,the ideal number of captured shrimp spawners should have ranged from 7 686 to 19 214 to maintain genetic diversity and effective population size. Further strategies to adjust the balance of economic cost, fishing effort and ideal number of shrimp spawners to maintain a satisfactory effective population size for ensuring the sustainability of Chinese shrimp are proposed.     

台湾海峡夏季与冬季的水体及热盐通量观测

利用在台湾海峡附近的下放式声学多普勒流速剖面仪(Lowered Acoustic Doppler Current Profiler,LADCP)观测资料和温盐观测资料,通过对连续站的两个季节观测进行正压和斜压潮流分析从而去除潮流得到准定常流,并在此基础上计算了南海和东海之间通过台湾海峡输运的水体及热盐通量。结果表明:台湾海峡大部分海域是半日潮海区(正规半日潮及不正规半日潮海区),半日潮主要分量为太阴半日分潮M2;台湾海峡的水体输运及热盐通量呈现明显的季节变化:夏季台湾海峡内表现为一支东北流向的海流,即台湾海峡暖流,存在3.3 Sv(1Sv=106 m3/s)的东北向水体输运,冬季东北季风较强,西南方向的海流加强,混合层可达到底部,存在1.8 Sv的东北向水体输运。与此对应的热盐通量分别为:夏季热通量为0.34×1015 W,盐通量为118.6×109 g/s;冬季热通量为0.14×1015 W,盐通量为72.9×109 g/s。该结果对台湾海峡通量的研究给出了一个直接观测的准确值,并为相关的数值研究提供了参考。     

汶川8.0级地震发震断层的累积地震位错研究

2008年5月12日,四川省汶川县内发生MS8.0地震。此次地震沿龙门山中央断裂产生1条长达200km的同震地表破裂带。文中选择位于地震地表破裂带北段的南坝镇、凤凰村以及南段的映秀镇这3个地点,以被断层错断的河流阶地为研究对象,对多级阶地面上的地震地表破裂及断层陡坎地貌进行了野外实测工作。经过测量数据的计算和分析,得到了各级阶地上断层陡坎的高度,该值即为该阶地记录的地震断层的累积垂直位错量。若以本次地震的垂直位错量作为古地震位错量的均值,则可计算得到每级阶地累积的地震次数。研究结果表明,各点T1阶地形成以来仅经历过1次事件,即本次地震事件;T2阶地形成以来约经历了5次事件;T3阶地形成以来约经历了9～11次事件;T4阶地形成以来约经历了20次事件。在本文研究的基础上,结合前人的阶地测年数据,则可获得古地震复发间隔的可靠数据