移植操作胁迫对天鹅湖大叶藻存活、生长及光合色素含量的影响

2009年4月利用植株枚订移植法在山东荣成天鹅湖海域开展大叶藻植株移植,于5~10月监测移植大叶藻植株的存活率,并逐月采集自然和移植大叶藻植株,比较了植株生长、叶绿素含量和类胡萝卜素含量的变化,分析了移植操作胁迫对大叶藻存活、生长、叶绿素含量和类胡萝卜素含量的影响。结果显示,移植后1~2个月,移植植株的平均成活率较高,至移植后4个月,平均成活率降至57.8%,之后保持稳定。移植后6个月内,叶长移植植株均极显著低于自然植株;根长和茎节长在移植后1个月时移植植株显著低于自然植株,移植2个月后与自然植株无明显差异,甚至显著高于自然植株;叶绿素含量和类胡萝卜素含量在移植后1个月时移植植株显著低于自然植株,移植2个月后则与天然植株无明显不同,甚至显著高于自然植株,但10月份移植植株的叶绿素含量明显下降。结果表明,移植操作对大叶藻植株的地上部分产生了严重的胁迫作用和较长的胁迫时间,而移植植株的地下部分及叶片叶绿素和类胡萝卜素在经过短期胁迫后,能够通过自身的补偿机制分别实现快速生长和显著增加,从而利于植株的扎根、固着和提高植株的光合作用能力。     

Effect of copper on the growth of shrimps Litopenaeus vannamei: water parameters and copper budget in a recirculating system

Shrimps( Litopenaeus vannamei) were intensively cultured in a recirculating aquaculture system for 98 days to investigate effects of 0.3 mg/L Cu on its performance, Cu budget, and Cu distribution. Shrimps in Cu-treated systems had greater mean final weight(11.10 vs 10.50 g), body length(107.23 vs 106.42 mm), survival rate(67.80% vs 66.40%), and yield(6.42 vs 5.99 kg/m 3), and lower feed conversion ratio(1.20 vs 1.29) than those in control systems but the differences were not significant. Vibrio numbers remained stable(10 4 –10 6 colony forming units/mL) in the rearing tanks of both control and treated systems. Total ammonium-N, nitrite-N, nitrate-N, pH, chemical oxygen demand, 5-day carbonaceous biochemical oxygen demand, and total suspended solids were similar in controls and treatments. Dissolved Cu concentration in the treated systems decreased from 0.284 to 0.089 mg/L while in the control systems it increased from 0.006 2 to 0.018 mg/L. The main sources of Cu in the treated systems were the artificially added component(75.7% of total input), shrimp feed(21.0%), water(2.06%), and shrimp biomass(1.22%). The major outputs of Cu occurred via the mechanical filter(41.7%), water renewal(15.6%), and draining of the sediment trap(15.1%). The foam fractionator removed only 0.69% of total Cu input. Harvested shrimp biomass accounted for 11.68% of Cu input. The Cu concentration of shrimps in the Cu-treated systems(30.70 mg/kg wet weight) was significantly higher than that in control systems(22.02 mg/kg). Both were below the maximum permissible concentration(50 mg/kg) for Cu in seafood for human consumption in China. Therefore, recirculating systems can be used for commercial on-growing of Litopenaeus vannamei without loss of shrimp quality, even in water polluted by 0.30 mg/L Cu. The mechanical filter is the main route for Cu removal.     

Spatiotemporal patterns of road network and road development priority in three parallel rivers region in Yunnan, China: An evaluation based on modified kernel distance estimate

Road network is a critical component of public infrastructure,and the supporting system of social and economic development.Based on a modified kernel density estimate(KDE)algorithm,this study evaluated the road service capacity provided by a road network composed of multi-level roads(i.e.national,provincial,county and rural roads),by taking account of the differences of effect extent and intensity for roads of different levels.Summarized at town scale,the population burden and the annual rural economic income of unit road service capacity were used as the surrogates of social and economic demands for road service.This method was applied to the road network of the Three Parallel River Region,the northwestern Yunnan Province,China to evaluate the development of road network in this region.In results,the total road length of this region in 2005 was 3.70×104km,and the length ratio between national,provincial,county and rural roads was 1∶2∶8∶47.From 1989 to 2005,the regional road service capacity increased by 13.1%,of which the contributions from the national,provincial,county and rural roads were 11.1%,19.4%,22.6%,and 67.8%,respectively,revealing the effect of′All Village Accessible′policy of road development in the mountainous regions in the last decade.The spatial patterns of population burden and economic requirement of unit road service suggested that the areas farther away from the national and provincial roads have higher road development priority(RDP).Based on the modified KDE model and the framework of RDP evaluation,this study provided a useful approach for developing an optimal plan of road development at regional scale.     

基于模糊小波网络的伺服系统参数辨识研究

伺服系统大多是非线性系统,难以对其建立准确的控制模型,其位置、和速度检测信号易受干扰,而小波网络具有多分辨率特性和任意逼近能力。利用其非线性映射能力对系统的输入输出关系进行模拟,将小波网络和模糊规则结合对系统的位置和速度进行辨识,动态调整网络的权值W和模糊规则,将非线性映射的问题转化为求解系统最优解,从而产生一种新的系统辨识方法,并以永磁伺服系统为例,设计了辨识的结构模型和策略,实验表明该算法可以达到较高的系统控制要求。     

颗粒态有机物在长江口及其邻近海域的夏季分布和影响因素初析

利用气相色谱法对长江口及其邻近海域的表层颗粒态木质素(p-lignin)进行测定和分析,结合粒度、有机碳(OC%)、叶绿素a(Chl a)、碳稳定同位素(δ13C)等参数研究颗粒态有机物的夏季分布,并对其分布影响因素进行了初步分析。结果表明,悬浮颗粒物粒度组成以粘土和粉砂为主,平均粒径为7.9μm;OC%的值为0.57—7.41%,Chl a的值为0.35—3.71μg/L,δ13C的值为-25.7‰—–16.6‰,在口门外水华站位出现OC%、Chl a和δ13C的最大值,表明浮游生物的现场生产是主要贡献;紫丁香基酚类(S)、香草基酚类(V)和肉桂基酚类(C)8种木质素酚单体的含量Λ8(相对于总有机碳的含量)为0.0406—1.47mg/100mg OC;紫丁香基系列与香草基系列的质量比值(S/V)的分布范围较宽,为0.5—1.6之间,均值为0.8;肉桂基含量与香草基含量比值(C/V)的分布范围为0.02—0.2之间,均值为0.09;香草基酚类的酸醛比值[(Ad/Al)v]在0.24—2.30之间。盐度、总悬浮颗粒物(TSM)浓度是控制长江口内区与邻近海域颗粒态有机物来源与分布差异的重要控制因素,颗粒态木质素在向海输送过程中还会受到矿物组分、生物降解、浮游生物现场生产等各种因素的作用,使其组成成分和性质发生改变。木质素等参数表明最大浑浊带尽管对颗粒态有机物向海输送有改造作用,但是影响区域有限。     

海山区浮游生态学研究

本文综述了海山浮游生态学研究的现状。海山是大洋中常见的地形单元,在有的海山附近鱼类和底栖生物的生物量比周围大洋中要多,针对这些鱼类和底栖生物的饵料来源提出了三种假说,第一种假说认为海山区特殊的物理环境导致初级生产力较高(经典假说),第二种假说认为海山区的饵料不是本地生产的,地形和流场使得外来的浮游生物在海山区富集或通量加大(外来营养补充假说),第三种假说认为海山上部的颗粒有机物营养较高,是鱼类和底栖生物的营养来源之一(颗粒有机物补充假说)。对海山浮游生态学的研究基本围绕这几个假说进行。目前只在9座海山进行了浮游生态学研究,这些研究表明单个假说并不能解释海山附近鱼类生物量较大的现象,海山鱼类和底栖生物的营养来源可能有很多,这些假说阐述的机制并不互相矛盾,海山生物之间的营养关系也比原来想象的复杂得多。已有研究都是针对其中某个假说进行验证研究,建议我国即将进行的海山浮游生态学研究针对三种假说进行海上调查,以提供完备的数据支持。     

论证南海海疆国界线

追溯探查历史图件,根据1946年内政部方域司编制的一组"南海诸岛位置图"(南海诸岛位置图,南沙群岛,中沙群岛,西沙群岛,西沙群岛永兴岛及石岛,南沙群岛太平岛)和1948年出版的"中华民国行政区域图"分析,南海诸岛外围的断续线段是我国在南海的海疆国界线;当时划定,是与陆域国界相连的,海上国界是陆域国界的延伸,以断续线段表示是国际地图上对海疆国界线通用的表示方法,当时即获国际认可。上述结果为南海划界提供了科学依据。     

Spatial distribution pattern of seafloor hydrothermal vents to the southeastern Kueishan Tao offshore Taiwan Island

Investigations of the diffusion activities both within and outside the seafloor hydrothermal vents, as well as related mineral genesis, have been one of the key focuses of ocean biogeochemistry studies. Many hydrothermal vents are distributed close to the southern Okinawa Trough on the less-than-30-m deep shallow seafloor off Kueishan Tao, northeast of Taiwan Island. Investigations of temperature, pH and Eh at four depths of hydrothermal plume were carried out near Kueishan Tao at the white(24.83°N, 121.96°E) and yellow(24.83°N, 121.96°E) vents. An 87 h of temperature time series observation-undertaken near the white vent showed that tide is the main factor affecting the background environment. Based on the observed data, 3-dimensional sliced diffusion fields were obtained and analyzed. It was concluded that the plume diffused mainly from north to south due to ebb tide. The yellow vent's plume could effect as far as the white vent surface. From the temperature diffusion field, the vortices of the plume were observed. The Eh negative abnormality was a better indicator to search for hydrothermal plumes and locate hydrothermal vents than high temperature and low pH abnormalities.     

基于三维荧光光谱-平行因子技术联用的浮游藻化学分类学技术研究

An in vivo three-dimensional fluorescence method for the determination of algae community structure was developed by parallel factor analysis(PARAFAC) and CHEMTAX. The PARAFAC model was applied to fluorescence excitation-emission matrix(EEM) of 60 algae species belonging to five divisions and 11 fluorescent components were identified according to the residual sum of squares and specificity of the composition profiles of fluorescent. By the 11 fluorescent components, the algae species at different growth stages were classified correctly at the division level using Bayesian discriminant analysis(BDA). Then the reference fluorescent component ratio matrix was constructed for CHEMTAX, and the EEM–PARAFAC–CHEMTAX method was developed to differentiate algae taxonomic groups. The correct discrimination ratios(CDRs) when the fluorometric method was used for single-species samples were 100% at the division level, except for Bacillariophyta with a CDR of 95.6%. The CDRs for the mixtures were above 94.0% for the dominant algae species and above 87.0% for the subdominant algae species. However, the CDRs of the subdominant algae species were too low to be unreliable when the relative abundance estimated was less than 15.0%. The fluorometric method was tested using the samples from the Jiaozhou Bay and the mesocosm experiments in the Xiaomai Island Bay in August 2007. The discrimination results of the dominant algae groups agreed with microscopy cell counts, as well as the subdominant algae groups of which the estimated relative abundance was above 15.0%. This technique would be of great aid when low-cost and rapid analysis is needed for samples in a large batch. The fluorometric technique has the ability to correctly identify dominant species with proper abundance both in vivo and in situ.     

中国贝类养殖对海洋碳循环的贡献评估

造成全球暖化的主要原因是温室气体的过量排放,其中CO2的贡献率达60 %,贝类养殖具有碳沉积作用。依据农业部渔业局编制的《中国渔业统计年签》,以2001年到2010年的年平均产量计算贝类捕获和养殖的碳沉积能力,并评估其碳沉积潜力;计算牡蛎、蛤、扇贝与贻贝四种贝壳单位面积的碳沉积能力并与森林、珊瑚礁的碳沉积能力进行比较分析。本文对我国浅海贝类养殖所具有的碳沉积能力进行评估,以了解贝类养殖对海洋碳循环的贡献,可为争取国家碳份额的合法权益提供基础数据。分析表明我国近十年贝类总产量稳定在1100万吨以上,并有增加的趋势,其中海水养殖贝类约占87.34 %。贝类养殖和捕获总产量的碳沉积和海水养殖产量的碳沉积量分别为58.57、51.15万吨/年,碳沉积能力分别相当于122.28、106.78万公顷的造林,可分别减少大气CO2增加量的0.0125 %、0.0109 %。牡蛎、蛤、扇贝与贻贝的单位面积碳沉积速率分别为1.573、0.388、0.301、1.039吨碳/(公顷?年);牡蛎和贻贝高于森林的碳沉积能力0.479吨碳/(公顷?年);但低于珊瑚礁的碳沉积能力1.8吨碳/(公顷?年)。我国贝类淡、海水养殖产量可分别创造约268.4万元/年、12,711.2万元/年的碳权商机。