东海和黄海表层沉积物生物标志物的分布特征及古生态重建潜力

多参数生物标志物法已被广泛用于重建浮游植物生产力和种群结构.但目前还缺乏这一应用的现代证据,尤其是西太平洋边缘海地区.文中作者对东/黄海的表层沉积物中的浮游植物生物标志物的分布进行研究,发现东海表层沉积物中菜子甾醇、甲藻甾醇、长链烯酮的总含量变化范围为357～3 115 ng/g,黄海的总含量变化范围为2 898 ～6 714 ng/g,硅藻、甲藻和颗石藻生物标志物总量基本具有近岸高、离岸低的趋势,与现场调查资料所得到的趋势基本一致.生物标志物比例结果显示在东海的长江口方向离岸越远硅藻相对比例下降,而在长江口以南则是离岸越远硅藻比例升高.在东海和黄海颗石藻所占比例都很低,但是有离岸越远比例升高的趋势.用生物标志物所重建的种群结构的变化趋势与现场调查资料相符,但其绝对值却与现场资料存在差异.本研究为生物标志物重建种群结构提供了现代数据,但是此方法的定量应用还需做进一步的研究.     

秋季南黄海网采浮游生物的生物量谱

对2006年9月南黄海浮游生物网(孔径为70,160,505μm)采集样品内的浮游生物个体大小的粒径分布进行研究,确定各粒级大小的功能群组成,建立2006年秋季调查水域网采浮游生物的生物量谱,比较分析三个特征水域(黄海近岸、黄海中部及黄海和东海交汇区)的浮游生物生物量谱特征参数的异质性。结果表明:三种网采浮游生物粒级范围主要包括100 pg/个~70 ng/个的浮游植物和70 ng/个~62 mg/个的浮游动物。Sheldon型生物量谱为近似连续的波动曲线,标准型生物量谱为线型。总测区的标准生物量谱斜率和截距为-0.74和18.64,各个特征水域,黄海中部为-0.67和15.60、黄海近岸为-0.64和14.34、黄海、东海交汇区为-0.73和18.03。浮游动物种类多样性对标准生物量谱的特征参数具有较显著的影响。     

复方清痹片糖衣片改薄膜衣片质量标准研究

目的：研究复方清痹片糖衣片改薄膜衣片的质量标准。方法：将原由滑石粉、糖浆、川蜡等辅料包糖衣片改为由胃溶型薄膜包衣预混剂包薄膜衣片,对薄膜衣片质量标准进行研究,对赤芍、防己、黄柏、甘草等进行薄层鉴别研究,并对赤芍中芍药苷进行含量测定方法学研究。结果：赤芍、防己、黄柏、甘草薄层鉴别斑点清晰,专属性强;芍药苷在2.9858～29.8584μg/ml之间有良好的线性关系,平均回收率98.33%,RSD%为1.70%。结论：糖衣片改薄膜衣片工艺可行,质量控制可靠、准确,可用于复方清痹片的质量控制。     

淡化浓海水日晒盐田原核微生物多样性分析

海滨日晒盐田具有稳定且独特的生态系统。淡化浓海水排入盐田制盐,对盐田生态系统的稳定及盐田的可持续发展具有一定的影响。本研究分析天津汉沽淡化浓海水日晒盐田不同季节不同盐度盐池原核微生物多样性现状及群落结构变化,以期为评估淡化浓海水对盐田原核微生物群落影响提供理论依据。采用温度梯度凝胶电泳和序列测定等方法,分析不同月份不同盐度盐池中细菌和古菌的多样性与群落结构变化。分别采用香农-威纳指数、加权丰富度指数和均匀度指数分析盐田微生物群落多样性、丰富度和均匀度。结果表明,淡化浓海水和溴后水中均未检测出细菌和古菌。不同月份不同盐度盐池样本中细菌多样性变化不大,优势菌主要为γ-变形菌门(γ-Proteobacteria)中的Spiribacter salinus和Pseudoalteromonassp.;古菌仅出现在盐度最高的Ⅶ号盐池中,优势古菌为广古菌门(Euryarchaeota)的Halogeometricum sp.。天津汉沽淡化浓海水日晒盐田中细菌的多样性和丰富度较低,春夏两季细菌群落结构较为稳定,秋冬季节细菌群落结构变化明显。盐度不同的盐池中细菌群落结构变化较大,随着盐度逐渐升高, Spiribacter salinus逐渐取代Pseudoalteromonas sp.成为占主要地位的优势菌种。     

利用卫星遥感海面温度、高度联合反演南海北部三维温度场

利用历史观测得到的温度剖面数据,通过严格筛选和插值,建立了南海北部的气候态垂向温度剖面。随后,利用回归统计分析的方法构建了海面温度异常(SSTA)、海面高度异常(SSHA)联合扩展温度剖面的经验回归模型,并采用卫星遥感得到的SST和SSH数据扩展了南海北部的三维海洋温度场,其时间分辨率为天,空间分辨率为0.25°×0.25°。通过与观测数据的对比研究,扩展得到的温度场可以较为准确地反映南海北部温度剖面的结构特征,并且能有效地体现出一些中尺度变化过程。结果表明,本研究反演得到的三维温度扩展场是较为可靠的,它可以作为海洋数值模型的初始场,实现现场观测数据和卫星遥感数据的互补,有助于更好地分析南海北部温度场的三维结构及变化特征。     

Photosynthetic response of floating Ulva prolifera to diurnal changes of in-situ environments on the sea surface

During yellow sea green tide outbreak, the thalli of Ulva prolifera drifted more than 350 km on the sea surface from off shore of Jiangsu Province to Qingdao city, where they were exposed to complex changes of local environments. The purpose of this study is to investigate the response of the thalli to diurnal changes of environments on the sea surface, and a sea surface environment simulator (SSES) was designed to simulate the natural environment of floating U . prolifera mat. A control experiment is designed in the laboratory, which was processed under suitable conditions (20°C for temperature, 72 μmol photons/(m 2 ·s) for light intensity and 30 for salinity), and an in-situ research was conducted to study the photosynthetic responses of floating U . prolifera to diurnal changes of environments. The results show that the photosynthetic efficiency of the thalli decreased gradually with time, and decreased rapidly after 14:00 local time (LT). After exposed to the environment on the sea surface for 6 h, the photosynthetic activity of the thalli decreased significantly. Furthermore, physiological-level and molecular-level experiments revealed that non-photochemical quenching (NPQ), cyclic electron flow (CEF) and energy redistribution between PSI and PSII all played an important role in the strong photosynthetic plasticity of U . prolifera . NPQ is the most important photoprotective responses to environmental changes before 12:00 LT. The results also confirmed that the CEF and energy redistribution between PSI and PSII are the main synergistic eff ects for the thalli to adapt to the environmental changes when the process NPQ cannot work. And the result can further reveal the reason why U . prolifera can adapt to the living condition of long distance drift on the sea surface. The findings of this research could provide a theoretical basis for explaining outbreaks of the green tide and instructing the management of the problem.     

黄海南部小黄鱼洄游分布及种群结构特征

Monthly fishery survey data of the small yellow croaker Larimichthys polyactis in the southern Yellow Sea from2003 and 2013 were employed to evaluate the variation in the resource distribution and biological characteristics(especially body length and sex ratio) in the population on a decadal scale.The results indicated that the small yellow croaker migrated from the Shawai fishing ground to the Dasha fishing ground in spring and was mainly distributed in the central and western parts of the Dasha fishing ground in April and May.Larimichthys polyactis in the Dasha fishing ground migrated eastward to offshore wintering grounds in autumn and reached the central Dasha fishing ground in October and November.The small yellow croaker entered the western waters of the Shawai fishing ground in winter.A large number of age 0+ fish occurred in the Shawai and Jiangwai fishing grounds in October of 2003 and 2013.The body lengths of the spawning stock and wintering stock in 2013 were larger than those in 2003,and the monthly sex ratios(female to male) were significantly less than 1 in both years.The monthly distribution of this fish in the southern Yellow Sea was consistent with a previous finding that "the stock migrated between the wintering grounds in the west of Jeju Island and the Lüsi spawning grounds" but tended to move more northward,with the spawning grounds extending outward.In the past decade,body length variation experienced a decline after an increase,rather than a steady decrease.The sex ratio in the single-stick stow net showed a tendency to increase over the decade,but was either less than or more than 1 depending on the fishing gear;therefore,further studies should be conducted to determine the sex structure.     

无居民海岛植被恢复过程中物种多样性及土壤特征初步研究

2016年4月对厦门无居民海岛猴屿开展了植被和土壤调查,采用全岛调查的方法查清全岛植物种类及生长状况,采用样方调查的方法分析比较人工恢复林和次生林物种多样性和土壤肥力特征,以期为无居民海岛生态修复提供借鉴.结果表明,全岛共有乔木5科5属6种,灌木有10科12属13种,草本植物有21科39属40种.由于被海包围、面积狭小、地形简单,物种传播速度慢,猴屿生物多样性较低,猴屿人工恢复林物种多样性与次生林差异不显著.人工恢复林土壤肥力显著低于次生林,植物生长及其产生的凋落物可以促进土壤肥力的改善.海岛普遍风大、缺水,植被恢复应选用适应海岛环境能力强的物种.猴屿植被破坏导致生态位缺失,进而造成马缨丹生物入侵,影响海岛生态系统稳定性.     

东海陆架盆地南部及邻近陆域中生代地层格架对比

在大量收集东海陆架盆地南部海域和邻近浙闽陆域中生界露头、钻井和地震资料的基础上,按由陆及海的思路,从构造、地层和沉积等方面入手,对海域和陆域中生界进行了划分及对比,探讨了这一区域的中生代地层格架和盆地类型,初步建立了东海陆架盆地南部及邻区中生代地层格架对比关系。东海陆架盆地南部中生代地层格架的厘定具有重要的理论和油气勘探实际意义。     

基于活动目录的通用授权管理平台的设计与实现

考虑应用系统中如何安全、高效的进行统一的人员管理及权限管理问题,研究基于微软活动目录构建通用中间件并以WebService方式发布的解决方案。具体内容包括把活动目录作为统一的人员库;构建通用授权管理平台统一管理用户在不同的软件系统中的角色和权限;该管理平台以WebService的方式向其它的应用系统提供接口。文中示例表明该方案切实可行,应用范围广。