A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians

To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.     

A Comparison of Simulated Particle Fluxes Using NEMURO and Other Ecosystem Models in the Western North Pacific

JGOFS has revealed the importance of marine biological activity to the global carbon cycle. Ecological models are valuable tools for improving our understanding of biogeochemical cycles. Through a series of workshops, the North Pacific Marine Science Organization (PICES) developed NEMURO (North Pacific Ecosystem Model Understanding Regional Oceanography) a model, specifically designed to simulate the lower trophic ecosystem in the North Pacific Ocean. Its ability to simulate vertical fluxes generated by biological activities has not yet been validated. Here compare NEMURO with several other lower trophic level models of the northern North Pacific. The different ecosystem models are each embedded in a common three-dimensional physical model, and the simulated vertical flux of POM and the biomass of phytoplankton are compared. The models compared are: (1) NEMURO, (2) the Kishi and Nakata Model (Kishi et al., 1981), (3) KKYS (Kawamiya et al., 1995, 2000a, 2000b), and (4) the Denman model (Denman and Peña, 2002). With simple NPZD models, it is difficult to describe the production of POM (Particulate Organic Matter) and hence the simulations of vertical flux are poor. However, if the parameters are properly defined, the primary production can be well reproduced, even though none of models we used here includes iron limitation effects. On the whole, NEMURO gave a satisfactory simulation of the vertical flux of POM in the northern North Pacific.     

Growth stimulation and inhibition of natural phytoplankton communities by model organic ligands in the western subarctic Pacific

The influence of organic ligands on natural phytoplankton growth was investigated in high-nitrate low-chlorophyll (HNLC) waters and during a phytoplankton bloom induced by a mesoscale iron enrichment experiment (SEEDS II) in the western subarctic Pacific. The growth responses of the phytoplankton in the treatments with iron complexed with model ligand were compared with those with inorganic iron or a control. Desferrioxamine B and protoporphyrin IX were used as models for hydroxamate-type siderophore and tetrapyrrole-type cell breakdown ligand, respectively. In the HNLC water, iron associated with protoporphyrin IX especially stimulated smaller phytoplankton (<10 μm) growth, 1.5-fold more than did inorganic iron. Surprisingly, only the addition of protoporphyrin IX stimulated small phytoplankton growth, suggesting that these cell breakdown ligands might be more bioavailable for them. The protoporphyrin IX’s stimulatory effect on small phytoplankton was not observed during bloom decline phase. The growth of phytoplankton was inhibited in the treatment with desferrioxamine B-complexed iron, suggesting its low bioavailability for the natural phytoplankton community. Its inhibitory effects were particularly pronounced in pico-eukaryotic phytoplankton. During the iron-induced bloom, the phytoplankton’s iron-stress response gradually increased with the desferrioxamine B concentration, suggesting that the competition for iron complexation between natural ligands and desferrioxamine B affected phytoplankton growth. However, the pico-eukaryotes did seem better able to utilize the desferrioxamine B-complexed iron during the bloom-developing phase. These results indicate that the iron bioavailability for phytoplankton differs between bloom-developing and bloom-decline phases.     

A search for millimeter wave spectral lines from comet Sugano-Saigusa-Fujikawa (1983e)

The search for radio spectral lines from Comet Sugano-Saigusa-Fujikawa (1983e) was conducted using the 45-m telescope of Nobeyama Radio Observatory. The frequency ranges of 44.0–46.0 and 47.5–49.5 GHz were surveyed down to $\text{ΔT}{}_{\mathrm{<mtext>A</mtext>}}{}^1\text{(}\text{rms}\text{) = 20–30}\text{mK}$, with a beam size of ～35 arc sec. Upper limits have been established for spectral lines of atomic hydrogen, CS, OCS, SO₂, H₂CO, CH₃OH, HCCCCCN, HCOOCH₃, CH₃OCH₃, and CH₃CH₂CN. The J = 5?4 line from HCCCN in the vibrational ground state possibly has been detected but not confirmed. The suggested total amount of HCCCN in the coma is consistent with the possible picture that HCCCN is the main parent molecule of CN.     

The multiple chronological techniques applied to the Lake Suigetsu SG06 sediment core,central Japan

The varved sediment of Lake Suigetsu (central Japan) provides a valuable opportunity to obtain high‐resolution, multi‐proxy palaeoenvironmental data across the last glacial/interglacial cycle. In order to maximize the potential of this archive, a well‐constrained chronology is required. This paper outlines the multiple geochronological techniques being applied – namely varve counting, radiocarbon dating, tephrochronology (including argon–argon dating) and optically stimulated luminescence (OSL) – and the approaches by which these techniques are being integrated to form a single, coherent, robust chronology. Importantly, we also describe here the linkage of the floating Lake Suigetsu (SG06) varve chronology and the absolute (IntCal09 tree‐ring) time scale, as derived using radiocarbon data from the uppermost (non‐varved) portion of the core. This tie‐point, defined as a distinct (flood) marker horizon in SG06 (event layer B‐07–08 at 1397.4 cm composite depth), is thus derived to be 11 255 to 11 222 IntCal09 cal. years BP (68.2% probability range).     

Fossil worm tubes from the presumed cold-seep carbonates of the Miocene Hayama Group, Central Miura Peninsula, Japan

Abstract Fossil worm tubes were collected from the Hayama Group, Miura Peninsula, Japan, together with abundant fossils of Calyptogena-Acharax clams. The fossil worm tubes were well preserved and coated with milky white amorphous silica. Most of the tubes were 1-3 mm in diameter, and up to 10 cm in length. Worm tubes were found in siltstone and limestone, and formed network-like assemblages. Elemental mapping on the tube cross-sections revealed the localization of sulfur, zinc and iron at the worm tubes, which suggests that sulfur-related metabolism and deposition occurred in association with the worm tubes. High resolution analysis revealed the localization of zinc-sulfur (sphalerite, ZnS) on the tubes, while iron-sulfur (pyrite, FeS₂) was localized at the center of the tubes. The spatially separate sphaleritization and pyritization imply that epiphytic and endosymbiotic microorganisms perform different sulfur metabolisms, such as sulfate-reduction and sulfide-oxidation.     

Dust Trails of 8P/Tuttle and the Unusual Outbursts of the Ursid Shower

We calculate the position of dust trails from comet 8P/Tuttle, in an effort to explain unusual Ursid meteor shower outbursts that were seen when the comet was near aphelion. Comet 8P/Tuttle is a Halley-type comet in a 13.6-year orbit, passing just outside of Earth's orbit. We find that the meteoroids tend to be trapped in the 12:14 mean motion resonance with Jupiter, while the comet librates in a slightly shorter period orbit around the 13:15 resonance. It takes 6 centuries to decrease the perihelion of the meteoroid orbits enough to intersect Earth's orbit, during which time the meteoroids and comet separate in mean anomaly by 6 years, thus explaining the 6-year lag between the comet's return and Ursid outbursts. The resonances also prevent dispersion along the comet orbit and limit viewing to only one year in each return. We identified past dust trail encounters with dust trails from 1392 (Dec. 1945) and 1378 (Dec. 1986) and predicted another outburst on 2000 December 22 at around 7:29 and 8:35 UT, respectively, from dust trails dating to the 1405 and 1392 returns. This event was observed from California using video and photographic techniques. At the same time, five Global-MS-Net stations in Finland, Japan, and Belgium counted meteors using forward meteor scatter. The outburst peaked at 8:06±07 UT, December 22, at zenith hourly rate ∼90 per hour, and the Ursid rates were above half peak intensity during 4.2 h. We find that most Ursid orbits do scatter around the anticipated positions, confirming the link with comet 8P/Tuttle and the epoch of ejection. The 1405 and 1392 dust trails appear to have contributed similar amounts to the activity profile. Some orbits provide a hint of much older debris being present as well. This work is the strongest evidence yet for the relevance of mean motion resonances in Halley-type comet dust trail evolution.