大都市区的形成机制及其定界：以北京为例

源于欧美的大都市区,在中国具有特殊的形成机制和联系形式。对北京的研究表明,其形成主要由中心城市工业化的扩散和农村非农业化两种过程所决定,并据此提出了中国大都市区定界的初步方法。进一步研究还发现中国已经出现了都市连绵带。     

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Dynamics and relationships of Ca, Mg, Fe in litter, soil fauna and soil in Pinus koraiensis-broadleaf mixed forest

The Liangshui Natural Reserve in Heilongjiang Province of China was selected as the study area. The authors collected the samples of forest litter （Tilia amurensis, Fraxinus mandshurica, Pinus koraiensis, Acer mono, Betula costata, and mixed litter）, soil in humus horizon （0--5cm） and soil horizon （5-20cm）, and soil macrofauna （Oligochaeta, Geophiloporpha and Juliformia） from 2001 to 2002. The role of soil macrofauna in the material cycle was analyzed through comparing the macro-element contents among various parts of the subsystems and using enrichment index （El）. The results indicate that dynamic changes of various litters are very complicated. The contents of Fe in each kind of litter increase firstly, and then decrease in the study period. The changes of macro-element contents are greater in the broad-leaf litter than in the coniferous litter, and the mixed litter is in the middle level, but the differences among them are not significant. The contents of Mg and Fe in humus are higher than those in soil, but the contents of Ca in soil are higher than that in humus. The dynamic changes of macro-element contents in soil and soil fauna are not consistent with those in litter. The diplopod presented obvious enrichment of Ca and Mg （E1〉1）, but it does not significantly enrich Fe. Earthworm has a stronger enrichment ability of Fe than diplopod and scolopendra, but E1〈1. Soil fauna can make great influences on the material cycle of the subsystems.     

RTK技术在老挝甘蒙省钾盐矿勘查区地形图修测中的应用

以老挝人民民主共和国甘蒙省钾盐矿勘查区1∶50000地形图修测项目实例,介绍GPS RTK技术的基本原理和工作流程,总结出这种技术的优势所在以及实践操作中应该注意到的问题。     

南极数值天气预报应用与研究进展

多个业务化的全球预报模式和有限区域预报模式应用于南极地区,已经成为南极天气预报和南极气候诊断研究的主要工具.现今南极地区的数值天气预报模式在模拟南极大气环流和天气系统方面性能不够理想,原因是多方面的:南极天气气候的特殊性(包括边界层的参数化、中尺度气旋等)、模式对南极复杂地形的描述不够充分、由于缺乏观测资料而使得模式的...     

Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

Methods for extraction of microorganism DNA from glacier surface snow

In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard- Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K (1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from glacier surface snow.     

Effect of copper on the growth of shrimps Litopenaeus vannamei: water parameters and copper budget in a recirculating system

Shrimps( Litopenaeus vannamei) were intensively cultured in a recirculating aquaculture system for 98 days to investigate effects of 0.3 mg/L Cu on its performance, Cu budget, and Cu distribution. Shrimps in Cu-treated systems had greater mean final weight(11.10 vs 10.50 g), body length(107.23 vs 106.42 mm), survival rate(67.80% vs 66.40%), and yield(6.42 vs 5.99 kg/m 3), and lower feed conversion ratio(1.20 vs 1.29) than those in control systems but the differences were not significant. Vibrio numbers remained stable(10 4 –10 6 colony forming units/mL) in the rearing tanks of both control and treated systems. Total ammonium-N, nitrite-N, nitrate-N, pH, chemical oxygen demand, 5-day carbonaceous biochemical oxygen demand, and total suspended solids were similar in controls and treatments. Dissolved Cu concentration in the treated systems decreased from 0.284 to 0.089 mg/L while in the control systems it increased from 0.006 2 to 0.018 mg/L. The main sources of Cu in the treated systems were the artificially added component(75.7% of total input), shrimp feed(21.0%), water(2.06%), and shrimp biomass(1.22%). The major outputs of Cu occurred via the mechanical filter(41.7%), water renewal(15.6%), and draining of the sediment trap(15.1%). The foam fractionator removed only 0.69% of total Cu input. Harvested shrimp biomass accounted for 11.68% of Cu input. The Cu concentration of shrimps in the Cu-treated systems(30.70 mg/kg wet weight) was significantly higher than that in control systems(22.02 mg/kg). Both were below the maximum permissible concentration(50 mg/kg) for Cu in seafood for human consumption in China. Therefore, recirculating systems can be used for commercial on-growing of Litopenaeus vannamei without loss of shrimp quality, even in water polluted by 0.30 mg/L Cu. The mechanical filter is the main route for Cu removal.     

湖北省潮汐形变观测异常及干扰识别

对2003—2013年湖北省数字化定点形变潮汐观测中的突跳、阶变、震颤等异常的分析发现,气象因素对形变潮汐观测的影响复杂:1)不同气压场控制影响形变观测的异常形态不同,但响应频率一致;2)台风对形变观测的秒值和分钟值数据在1～4秒和2～4分钟较高频段均有响应;3)台址条件不同,降雨对形变观测产生的畸变影响不同。影响范围一般在0～全日波频段且存在较大的噪声;4)短时大气温度变化对形变观测有一定影响,会使曲线发生抖动或暂态波动,在64～128分钟和128～256分钟较低频段上有响应。水库冲沙、水库蓄水、土石开挖等环境荷载变化对分钟值观测曲线产生明显阶跃、抖动型实时畸变。人员进洞、施工、仪器标定检修等人为活动对观测曲线的影响可直接识别。     

走滑断裂带中挤压阶区内部剪切构造初探

野外地质调查和航片立体镜下解译发现,小江断裂带北段蒙姑和东支大营盘两个挤压阶区内部发育有剪切构造,它们在地表主要表现为断裂槽地、断层陡坎、地裂缝等。挤压阶区内部剪切构造和拉张阶区内部剪切构造一样,是阶区形成后,随着走滑断裂的进一步活动而形成的新生性构造