Temporal and geographical trends in the genotoxic effects of marine sediments after accidental oil spill on the blood cells of striped beakperch (Oplegnathus fasciatus)

To determine the impact of genetic toxicity caused by the Hebei Spirit oil spill on December 7, 2007, we measured DNA damage in the blood cells of striped beakperch in vitro after exposure to extracts from sediments in the Taean area. The objective of this study was to investigate temporal changes of toxic effects caused by residual PAHs in the sediments up to 18 months after an oil spill. In conclusion, DNA damage had reduced over this 18-month period; that is, the sediments recovered quickly from the oil pollution. In addition, statistically significant correlations between PAHs and DNA damage were observed. Because the comet assay is sensitive to DNA damage induced by genotoxic substances from the polluted sediments, the comet assay can be considered a useful tool as a biomarker in investigating genetic toxicity in environmental monitoring and elucidating the recovery of oil pollution after oil spill as well.     

Hebei Spirit oil spill monitored on site by fluorometric detection of residual oil in coastal waters off Taean, Korea

The spatiotemporal distributions of dissolved and/or dispersed oil in seawater and pore water were monitored on site by fluorometric detection method after the Hebei Spirit oil spill. The oil concentrations in intertidal seawater, 15 days after the spill, were as high as 16,600 μg/L and appeared to decrease below the Korean marine water quality standard of 10 μg/L at most sites 10 months after the spill. Fluorometric detection of oil in pore water was introduced to eliminate the effects of grain size for the quantification of oil in sediments and to better explain spatial and temporal distribution of oil pollution at sandy beaches. The fluorescence detection method was compared with the conventional laboratory technique of total petroleum hydrocarbon analysis using gas chromatography. The method of fluorescence detection of oil was capable of generating results much faster and more cost-effectively than the traditional GC technique.     

Assessment of butyl- and phenyltin pollution in the coastal environment of Korea using mussels and oysters

Butyl- and phenyltin residues in bivalves were quantified around the coast of Korean peninsula in 1995–1998 and 2001. Butyltin compounds were detected at all the sites surveyed, whereas phenyltins were detected at 41 out of 67 sites in 1995–1998. Tributyltin (TBT) concentrations in bivalves ranged 48–2800 ng Sn/g on dry weight basis. Triphenyltin (TPT) concentrations ranged <3–1820. The mean TPT concentration was 3.5 times less than that of TBT. Spatial distribution of TBT was closely related to boating and dry-docking activities. TPT concentration in bivalves showed significant correlation with TBT. Concentrations of TBT in bivalves, 2001 are not significantly changed compared to those in 1995–1998. However, TPT concentrations in bivalves steeply decreased in 2001. High concentration of TBT and lesser extent TPT and their nation-wide distribution indicate that organotins are one of major organic pollutants in the marine environment of Korea.     

SWAT application to estimate design runoff curve number for South Korean conditions

Runoff estimations based on the standard USDA–NRCS curve number (CN) table without calibration have a tendency to give inaccurate results when the CN values are applied in South Korea which has many high slope watersheds and that has a continental monsoon climate. Particularly for the design flood estimation, accurately calibrated CN values are required because the estimated peak flow is very sensitive to the selection of CN. However, the lack of flood data makes it difficult to calibrate and assign runoff CNs to Korean watersheds. Even if sufficient data are available to estimate CN values, it is also difficult to obtain the direct flows by separating base flows from total runoff hydrographs due to the temporal irregularity of rainfall events and the resulting complex pattern of runoff. Therefore, an alternative method for estimating CNs needs to be developed to overcome these issues. The purpose of this study is to present a method for estimating runoff CNs using the soil and water assessment tool (SWAT) model which can take into account watershed heterogeneities such as climate conditions, land use and soil types. The proposed CN estimation method uses the simulated flow data by SWAT instead of using measured flow data. This method has advantages in estimating CN values spatially for each subbasin division considering watershed characteristics. The use of daily data can reduce the sensitivity to the abnormality that is commonly involved in flow data with a small time scale. The SWAT‐based CN estimation method, combined with the asymptotic CN method, was applied to the Chungju dam watershed in South Korea. A regression equation was then developed from this approach, which was used to estimate CN values that decrease exponentially as rainfall amounts increase and that converge to 60·6 and 79·4 without and with considering subsurface lateral flow, respectively. Furthermore, the CN values for the antecedent moisture conditions were determined using the probabilistic approach. The CN associated with the 50% probability for the Chungju dam watershed is 87·8 which can be taken to be representative of antecedent moisture condition (AMC) II. The CN_I and CN_III associated with 90% and 10% probabilities are 78·9 and 94·1, respectively. The estimated CN_II = 87·8 differs markedly from the geographic information system (GIS)‐based CN 65·0, which implies that the standard USDA–NRCS CN method should be calibrated to the studied area of interest. Copyright © 2010 John Wiley & Sons, Ltd.     

Characterization of cholinesterases in marbled sole, Limanda yokohamae, and their inhibition in vitro by the fungicide iprobenfos

Cholinesterases (ChEs) have been characterized in marbled sole (Limanda yokohamae) for use as a possible biomarker of pollution exposure. In brain, ChEs existed almost exclusively (>95%) as acetylcholinesterase (AChE) whereas in muscle, about 20-30% of ChE activity was in the form of butyrylcholinesterase (BChE; pseudocholinesterase). Acetylthiocholine and butyrylthiocholine (identified in mammalian studies as diagnostic substrates for AChE and BChE respectively) were hydrolyzed mainly, but not exclusively, by these enzymes. The inhibitors BW284C51 and iso-OMPA (identified in mammalian studies as diagnostic inhibitors of AChE and BChE respectively) were not specific for these enzymes in marbled sole. Brain AChE and muscle AChE and BChE were characterized in terms of their kinetic properties (KM etc.) and optimal conditions (substrate concentration, protein concentration, pH etc.) were established to allow routine assays of ChE activity to proceed under pseudo-first order conditions. The sensitivity of ChEs to a locally significant pesticide, iprobenfos (IBP; kitazin) was established in terms of IC50 concentrations. Brain AChE was relatively insensitive to IBP, but muscle AChE and BChE were sensitive to IBP concentrations in the high nM range. However, ambient IBP concentrations in Korean coastal waters are usually not high enough to cause detectable ChE inhibition in this species.     

Application of a fuzzy operator to susceptibility estimations of coal mine subsidence in Taebaek City,Korea

Quantitative determination of locations vulnerable to ground subsidence at mining regions is necessary for effective prevention. In this paper, a method of constructing subsidence susceptibility maps based on fuzzy relations is proposed and tested at an abandoned underground coal mine in Korea. An advantage of fuzzy combination operators over other methods is that the operation is mathematically and logically easy to understand and its implementation to GIS software is simple and straightforward. A certainty factor analysis was used for estimating the relative weight of eight major factors influencing ground subsidence. The relative weight of each factor was then converted into a fuzzy membership value and integrated as a subsidence hazard index using fuzzy combination operators, which produced coal mine subsidence susceptibility maps. The susceptibility maps were compared with the reported ground subsidence areas, and the results showed high accuracy between our prediction and the actual subsidence. Based on the root mean square error and accuracy in terms of success rates, fuzzy γ-operator with a low γ value and fuzzy algebraic product operator, specifically, are useful for ground subsidence prediction. Comparing the results of a fuzzy γ-operator and a conventional logistic regression model, the performance of the fuzzy approach is comparative to that of a logistic regression model with improved computational. A field survey done in the area supported the method’s reliability. A combination of certainty factor analysis and fuzzy relations with a GIS is an effective method to determine locations vulnerable to coal mine subsidence.     

The effect of ozone and aerosols on the surface erythemal UV radiation estimated from OMI measurements

Surface erythemal UV radiation is mainly affected by total column ozone, aerosols, clouds, and solar zenith angle. The effect of ozone on the surface UV radiation has been explored many times in the previous studies due to the decrease of ozone layer. In this study, we calculated the effect of aerosols on the surface UV radiation as well as that of ozone using data acquired from Ozone Monitoring Instrument (OMI). First, ozone, aerosol optical depth (AOD), and surface erythemal UVB radiation measured from satellite are compared with those from ground measurements. The results showed that the comparison for ozone was good with r ² of 0.92. For aerosol, there was difference between satellite measurements and surface measurements due to the insufficient information on aerosol in the retrieval algorithm. The r ² for surface erythemal UV radiation was high (～0.94) but satellite measurements showed about 30% larger values than surface measurements on average by not considering the effect of absorbing aerosols in the retrieval process from satellite measurements. Radiative amplification factor (RAF) is used to access the effect of ozone and aerosol quantitatively. RAF for ozone was 0.97～1.49 with solar zenith angle. To evaluate the effect of aerosol on the surface UV radiation, only clear-sky pixel data were used and solar zenith angle and total column amount of ozone were fixed. Also, RAF for aerosol was assessed according to the single scattering albedo (SSA) of aerosols. The results showed that RAF for aerosol with smaller SSA (< 0.90) was larger than that for with larger SSA (> 0.90). The RAF for aerosol was 0.09～0.22 for the given conditions which was relatively small compared to that for ozone. However, considering the fact that aerosol optical depth can change largely in time and space while the total column amount of ozone does not change very much, it needs to include the effect of aerosol to predict the variations of surface UV radiation more correctly.     

The effect of grain size on the viscosity and yield stress of fine-grained sediments

In debris flow modelling,the viscosity and yield stress of fine-grained sediments should be determined in order to better characterize sediment flow.In particular,it is important to understand the effect of grain size on the rheology of fine-grained sediments associated with yielding.When looking at the relationship between shear stress and shear rate before yielding,a high-viscosity zone（called pseudoNewtonian viscosity） towards the apparent yield stress exists.After yielding,plastic viscosity（called Bingham viscosity） governs the flow.To examine the effect of grain size on the rheological characteristics of fine-grained sediments,clay-rich materials（from the Adriatic Sea,Italy; Cambridge Fjord,Canada; and the Mediterranean Sea,Spain）,silt-rich debris flow materials（from La Valette,France） and silt-rich materials（iron tailings from Canada） were compared.Rheological characteristics were examined using a modified Bingham model.The materials examined,including the Canadian inorganic and sensitive clays,exhibit typical shear thinning behavior and strong thixotropy.In the relationships between the liquidity index and rheological values（viscosity and apparent yield stress）,the effect of grain size on viscosity and yield stress is significant at a given liquidity index.The viscosity and yield stress of debris flow materials are higher than those of low-activity clays at the same liquid state.However the viscosity and yield stress of the tailings,which are mainly composed of silt-sized particles,are slightly lower than those of low-activity clays.