Antialgal and antilarval activities of bioactive compounds extracted from the marine dinoflagellate <Emphasis Type="Italic">Amphidinium carterae</Emphasis>

With the global ban on the application of organotin-based marine coatings by the International Maritime Organization, the development of environmentally friendly, low-toxic and nontoxic antifouling compounds for marine industries has become an urgent need. Marine microorganisms have been considered as a potential source of natural antifoulants. In this study, the antifouling potential of marine dinoflagellate Amphidinium carterae, the toxic and red-tide microalgae, was investigated. We performed a series of operations to extract the bioactive substances from Amphidinium carterae and tested their antialgal and antilarval activities. The crude extract of Amphidinium carterae showed significant antialgal activity and the EC₅₀ value against Skeletonema costatum was 55.4 μg mL^?1. After purification, the isolated bioactive substances (the organic extract C) exhibited much higher antialgal and antilarval activities with EC₅₀ of 12.9 μg mL^?1 against Skeletonema costatum and LC₅₀ of 15.1 μg mL^?1 against Amphibalanus amphitrite larvae. Subsequently, IR, Q-TOFMS, and GC-MS were utilized for the structural elucidation of the bioactive compounds, and a series of unsaturated and saturated 16- to 22-carbon fatty acids were detected. The data suggested the bioactive compounds isolated from Amphidinium carterae exhibited a significant inhibiting effect against the diatom Skeletonema costatum and Amphibalanus amphitrite larvae, and could be substitutes for persistent, toxic antifouling compounds.     

胶东金翅岭金矿构造控矿规律及找矿预测

金翅岭金矿是胶东招远北部的一个典型石英脉型金矿,构造是金矿最为重要的控矿因素。在前人资料的基础上,综合分析了矿区构造控矿特征,总结了构造控矿规律:矿脉分支复合,表现为NE向矿脉产生SN,NNE向次级矿脉,NNE向矿脉产生NE向次级矿脉;矿体厚度与金品位呈负相关,深部次级矿脉群在P4脉下盘出现;矿脉侧伏角和倾伏向变化处矿体富集,表现为SW向侧伏的P4矿脉,矿体富集于-240 m,侧伏角变大,同时倾伏向由SW向转变为NW向的部位。最后建立了平面和剖面P4矿脉的波形函数,并对深部进行了预测。     

Safety Assessment of Buried Pipeline during Pile Driving Vibration in Offshore Engineering

To assess the impact of pile driving on adjacent submarine pipelines during the reconstruction of a pier berth, the local damage model of submarine pipelines is established to explore the safety thresholds of the particle peak velocity and horizontal displacement. The results are analyzed and adjusted by the existing standards and the corresponding literatures. Then, a three-dimensional numerical model is presented to assess the feasibility of the construction of piles by the obtained safety limits, in which the nonlinear behavior of the soil and stress–seepage coupling analysis are considered. After the construction, the safety of submarine pipelines is rechecked by the measured value of the particle peak velocity and horizontal displacement. Meanwhile, the propagation law of vibration, the horizontal displacement of underground soil, and the pore pressure during pile driving are explored. The results indicate that the construction of piles of 2# mooring pier did not cause damage to adjacent submarine pipelines. However, the construction of piles of 1# mooring pier which is nearer may cause damage to submarine pipelines.     

大兴安岭十八站—韩家园地区晚中生代火山岩年龄、地球化学特征及其构造意义

大兴安岭十八站—韩家园地区发育晚中生代基性-中酸性火山岩。选取粗面安山岩、粗面岩、流纹岩进行年代学和岩石地球化学研究。粗面安山岩LA-ICP-MS锆石U-Pb年龄为125.2±0.9 Ma,为早白垩世火山作用的产物。岩石地球化学特征表明,中酸性火山岩属于高钾钙碱性-钾玄岩系列。岩石稀土元素总量∑REE=121.42×10~(-6)~154.43×10~(-6),轻、重稀土元素分异明显(La/Yb)_N=19.25~31.98,在稀土元素配分图上显示右倾型,除一个流纹岩样品具显著负Eu异常外,多数样品无明显Eu负异常(δEu=0.45~0.90)。岩石具弱富集大离子亲石元素Ba、Sr,明显亏损高场强元素Nb、Ta、Ti的特征。结合区域资料,认为十八站—韩家园地区早白垩世中酸性火山岩形成于蒙古-鄂霍茨克洋闭合机制下后碰撞伸展背景。粗面安山岩、粗面岩和流纹岩系同源岩浆部分熔融与结晶分异作用的结果,岩浆来源于受俯冲流体交代的富集岩石圈地幔。     

Comparison of single- and dual-permeability models in simulating the unsaturated hydro-mechanical behavior in a rainfall-triggered landslide

Landslide-prone slopes in earthquake-affected areas commonly feature heterogeneity and high permeability due to the presence of cracks and fissures that were caused by ground shaking. Landslide reactivation in heterogeneous slope may be affected by preferential flow that was commonly occurred under heavy rainfall. Current hydro-mechanical models that are based on a single-permeability model consider soil as a homogeneous continuum, which, however, cannot explicitly represent the hydraulic properties of heterogeneous soil. The present study adopted a dual-permeability model, using two Darcy-Richards equations to simulate the infiltration processes in both matrix and preferential flow domains. The hydrological results were integrated with an infinite slope stability approach, attempting to investigate the hydro-mechanical behavior. A coarse-textured unstable slope in an earthquake-affected area was chosen for conducting artificial rainfall experiment, and in the experiment slope, failure was triggered several times under heavy rainfall. The simulated hydro-mechanical results of both single- and dual-permeability model were compared with the measurements, including soil moisture content, pore water pressure, and slope stability conditions. Under high-intensity rainfall, the measured soil moisture and pore water pressure at 1-m depth showed faster hydrological response than its simulations, which can be regarded as a typical evidence of preferential flow. We found the dual-permeability model substantially improved the quantification of hydro-mechanical processes. Such improvement could assist in obtaining more reliable landslide-triggering predication. In the light of the implementation of a dual-permeability model for slope stability analysis, a more flexible and robust early warning system for shallow landslides hazard in coarse-textured slopes could be provided.