扇贝外套腔内的共栖生纤毛虫 Ⅰ.蠕状康纤虫(Cohnilembus verminus)的形态学研究

研究扇贝外套腔内栖生的纤毛虫一蠕状康纤虫Cohnilembusverminus（Muller，1786）的形态、核器及纤毛图式。结果显示其口器结构为；M1呈单列结构，约占体长的1／2，M2由4组毛基粒构成，PM起自M2末端，Sc约由5个毛基粒构成。此外，还列表比较了不同作者报导的C．verminus种群及其它6种已知的康纤虫的主要性状特征。     

南海深海盆地沉积柱样中的浊流沉积

本文根据南海深海盆地三个沉积柱样的粒度结构、地球化学、微体古生物等特征分析,深讨了南海深海盆地细粒沉积物的浊积现象。结果表明,位于南海北部陆架斜坡上KL37孔的浊流沉积现象并不明显;位于陆架斜坡和深海盆地交界处的KL29孔存在着大量的浊积层,属于浊流沉积和半远洋沉积环境;位于南海盆地中部的KL91孔虽然已属于远洋性沉积环境,但除出现火山灰沉积外,浊流沉积作用仍然是相当活跃的。     

黄河入海泥沙对渤海和黄海沉积作用的影响

渤海平均水深18m，最大水深70m，是一个较浅的海湾。黄海平均水深38m，其中部为水深大于50m的盆地。它们的共同特征是陆地环绕。沿岸河流输沙特征对沉积作用过程有重大影响，特别是黄河入海泥沙的影响尤为显著。 关于黄河人海泥沙对渤海和黄海沉积作用的影响，前人已从不同角度进行过讨论。黄河是世界上著名的多沙性河流。由于黄河尾闾长期以来不断发生变迁，纵横摆动于华北平原之上，交替注入渤海和黄海，因此，在讨论黄河入海泥沙在中国近海沉积中的作用时，必须把渤海和黄海作为一个整体，甚至南涉东海。本文试图在以往工作的基础上，概括讨论黄河入海泥沙对渤海和黄海海底沉积作用的影响。     

中国沿岸水域养殖贝类及其养殖笼网污损苔虫7新种

拟小孔苔虫属Microporella和斑孔苔虫属Fenestrulina均隶于唇口目Cheilostomida有囊亚目Ascophorina皮壳次目 Lepraliomorpha裂孔超科Schizoporelloidea拟小孔苔虫科Microporellidae。这两个属的主要区别在于：拟小孔苔虫属具有鸟头体，而斑孔苔虫属无鸟头体。我们在研究中国沿岸水域养殖贝类及其养殖笼网的污损苔虫时发现，中国一些学者以前所报道的“纤毛拟小孔苔虫”并非 Pallas(1766)所鉴定的种，而是包括不同于纤毛拟小孔苔虫Microporella ciliata Pallas，1766的5个不同的独立种，即本文所描述的拟小孔苔虫属的5个新种：空穴拟小孔苔虫 Microporella vacuatus sp.nov.、小筛网拟小孔苔虫Microporella cribellata sp.nov.、无齿拟小孔苔虫Microporella inermis sp.nov.、异北方拟小孔苔虫 Microporella antiborealis sp.nov.和项链拟小孔苔虫 Microporella monilifera sp.nov.。这5个新种与纤毛拟小孔苔虫的主要区别是:5新种的口刺为2~6根，除无齿拟小孔苔虫的口上卵胞仅具一列边缘孔外，其他4新种卵胞前表面还有分散的穿孔；而纤毛拟小孔苔虫的口刺为6根，口上卵胞细颗粒状，无穿孔。研究中我们还发现前人所定名为“马氏斑孔苔虫”的种并非 Audouin(1826)所描述的种，而是4种不同于马氏斑孔苔虫 Fenestrulina mallusii Audouin，1826的独立种，其中两种不属于污损苔虫的范畴，另外的两种即本文所描述的斑孔苔虫属的两新种，即中华斑孔苔虫Fenestrulina Sinica sp.nov.和东方斑孔苔虫Fenestrulina orientalis sp.nov.。这两个新种与马氏斑孔苔虫的主要区别在于：两新种口刺均为4~6根，受孕个虫有2根裸露的口刺；而马氏斑孔苔虫的口刺为2~3根，受孕个虫无裸露的口刺。     

Nitrogen forms and decomposition of organic carbon in the southern Bohai Sea core sediments

Study on form characteristics of nitrogen in marine sediments is the primary method to research its biogeochemical cycling and nitrogen form characteristics in core sediments can reflect the process and results of early diagenesis in a certain degree. In this paper, Sequential extraction process in natural grain size was used for studying the existent forms of nitrogen in five core sediments of the southern Bohai Sea for the first time. Nitrogen was divided into two parts -transferable and fixed based on whether it could be extracted by the reagent. Distributions and early diagenesis of transferable nitrogen forms in the southern Bohai Sea were researched integratedly. Results indicate that IEF - N and OSF-N are predominant forms in transferable part in the studied core sediments. Contents of different nitrogen forms vary differently with depth, and have different diagenesis process. Decomposition constant of organic nitrogen (ON) and OC are about 15.51 × 10~(-3)a~(-1)and 4.79× 10~(-3)a~(-1) respective     

东海虾类的生态群落与区系特征

有关东海的虾类，刘瑞玉(1959，1963，1964)、董聿茂(1959，1980，1986)等已做过大量的调查研究。近10多年来，由于东海传统的主要经济鱼类资源衰退，捕食虾类的鱼类减少，因此，使虾类生存空间扩大，这有利于虾类资源的繁衍生长，使虾类资源发生量增多，数量增长较快。东海区三省一市近几年虾类产量达到80×104~90×104t，其中浙江省为60×104~70×104t，拖虾作业已成为东海区一大作业方式，对促进海洋捕捞业的发展起重要作用。20世纪80年代中后期，我们开展了东海虾类资源开发调查，尔后又继续与拖虾生产船相结合进行监测调查，积累了一些资料，本文就东海虾类生态群落结构与区系特征进行探讨。     

乳糖诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichia coli)中的表达

采用廉价的乳糖替代IPTG诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichiacoli)JM109中表达,对诱导产物表达的培养基、培养条件、诱导剂的浓度和诱导时机进行了优化,将优化条件用于5L自控发酵罐进行高密度培养。结果表明,对rAPC表达的条件进行优化后,乳糖诱导rAPC的表达率可达26.8%,与IPTG的诱导结果接近;在高密度培养中,OD600最大达21.8。研究结果可为乳糖替代IPTG大规模生产rAPC奠定基础。     

锯缘青蟹NAGase的催化机理的判断

以p-硝基苯酚-β-D-氨基葡萄糖苷（pNP-NAG）为底物，研究产物类似物：p-羧基苯酚和苯酚对青蟹N-乙酰-β-D-氨基葡萄糖苷酶（NAGase，Ec3．2．1．52）活力的影响．结果表明：p-羧基苯酚和苯酚对该酶有抑制的作用，IC50分别为20．0和100．0mmol／dm^3．p-羧基苯酚和苯酚对酶的抑制均表现为竞争性类型，其抑制常数K1分别为5．03和25．67mmol／dm^3．结合产物N-乙酰-β-D-氨基葡萄糖（NAG）抑制作用表现为反竞争性类型，判断NAGase水解pNP-NAG反应为有序双双反应（Ordered Bi Bi）．     

Mixed layer variability in Northern Arabian Sea as detected by an Argo float

Seasonal evolution of surface mixed layer in the Northern Arabian Sea (NAS) between 17° N–20.5° N and 59° E-69° E was observed by using Argo float daily data for about 9 months, from April 2002 through December 2002. Results showed that during April - May mixed layer shoaled due to light winds, clear sky and intense solar insolation. Sea surface temperature (SST) rose by 2.3 °C and ocean gained an average of 99.8 Wm⁻². Mixed layer reached maximum depth of about 71 m during June - September owing to strong winds and cloudy skies. Ocean gained abnormally low ∼18 Wm⁻² and SST dropped by 3.4 °C. During the inter monsoon period, October, mixed layer shoaled and maintained a depth of 20 to 30 m. November - December was accompanied by moderate winds, dropping of SST by 1.5 °C and ocean lost an average of 52.5 Wm⁻². Mixed layer deepened gradually reaching a maximum of 62 m in December. Analysis of surface fluxes and winds suggested that winds and fluxes are the dominating factors causing deepening of mixed layer during summer and winter monsoon periods respectively. Relatively high correlation between MLD, net heat flux and wind speed revealed that short term variability of MLD coincided well with short term variability of surface forcing.