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271.
Diagnosis of iridovirus in large yellow croaker by PCR 总被引:1,自引:0,他引:1
A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection. 相似文献
272.
The effects of scattering and resonance on the energy dissipation of an internal tide were investigated using a two-dimensional
model which is a reassembled version of the theoretical generation model devised by Rattray et al. (1969) for internal tide. The basic character of the scattering process at the step bottom was first investigated with a
wide shelf model. When the internal wave incited from a deep region (Region II) into the shallow shelf region (Region I),
a passing wave into the shallow region, a reflected wave into the deep region, and a beam-like wave, i.e. a scattered wave
(SW), emanated at the step bottom. The SW, which consists of the superposition of numerous internal modes, propagated upward/downward
into both regions. The general properties of the SW were well expressed around the shelf edge, even in the present model with
viscosity effect. The amplitude of the SW decreased dramatically when the depth of the velocity maximum of the incident internal
wave in Region II corresponded with the depth of the shelf edge. In the narrow shelf model, where the decay distance of the
internal wave in Region I is longer than the shelf width, the incident internal wave reflected at the coast to form a standing
wave. When the internal wave in Region I is enhanced by the resonance, the energy of the SW in Region II is also intensified.
Furthermore, the energy of the modes in Region II predominated when the velocity maximum is identical to that of the dominant
mode in Region I. These results suggest that the spatial scale of shelf region is a very important factor governing the energy
dissipation of the internal tide through reflection and scattering in a narrow shelf. 相似文献
273.
本文从地质地层结构、新构造运动和人为(过量抽取地下热水)两方面因素探讨地面沉降的形成机理,并提出控制地面沉降的对策。 相似文献
274.
INTRODUCTIONCryptomonasisunicellularbiflagellatewhichisacommongenusofCryptophyta .Thecellsareusuallystronglycompressedlaterally .ThereareoneortwolargeChloroplaststhatcontainchlorophylla ,c ,somecharacteristiccarotenoidsandphycobilinpigments (Huetal.,1 997;Wei… 相似文献
275.
An experimental set-up is developed and proved to be effective for laboratory study of an underwater towed system. The experimental technique gives a practical method for monitoring the kinematic and dynamic performance of an underwater towed system in a ship towing tank. Both the theoretical and experimental results in the investigation indicate that the hydrodynamic response of a towed vehicle to the wave induced motion of a towing ship can be significantly reduced by applying a two-part tow method. A comparison of the numerical and experimental results in the investigation demonstrates that the numerical simulation results are close to the experimental data, overall agreement between experimental and theoretical results is satisfactory. The results qualitatively verify the mathematical model of a two-part underwater towed system proposed by Wu and Chwang [Wu, J., Chwang, A.T., 2000. A hydrodynamic model of a two-part underwater towed system. Ocean Engineering 27 (5), 455–472]. 相似文献
276.
盐藻和β-胡萝卜素研究述评 总被引:9,自引:1,他引:9
养殖盐藻生产β-胡萝卜素是近十几年新兴起的研究领域,因其应用前途广内,现已成为微藻生物学的研究前沿和热点,预计本世纪末和下世纪初将有更大发展。鉴于我国在这方面文章不系统、不全面,本文从盐藻生物学、养殖学、β-胡萝卜素异构体、β-胡萝卜素形成生理等角度全面概述了该领域的研究现状,并提出存在的问题与研究方向。 相似文献
277.
278.
279.
二色桌片参酶解液对转化细胞的效应 总被引:3,自引:2,他引:3
用二色桌片参(Mensamariaintercedens)酶解液处理转化的人胚肺成纤维细胞(NLF)后,细胞生长受到显著抑制,抑制率达64.84%,光镜下细胞透光性加强,铺展较好,细胞核形更为规则,核畸形现象减少;线粒体结构趋于正常;软琼脂集落实验表明,细胞集落形成率由23x10-5下降至12.5x10-5,裸小鼠致瘤实验显示抑瘤率为82.39%,差异显著(p<5%)。结果提示,二色桌片参酶解液可能逆转转化的HLF细胞的恶性表型,对其有一定的诱导分化作用。 相似文献
280.
Aproposeddirectionalfunctionandwind-wavedirectionalspectrum¥WenShengchang;WuKejian;GuanChanglong;SunShicaiandZhangDacuo(Recei... 相似文献