Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Current and thermohaline characteristics of the Arabian Sea during January 1998

Based on a ship survey during January 1998, the characteristics of the flow, the thermohaline properties and the volume transport of the Arabian Sea are discussed. A strong westward flow exists between 10.5?N and 11?N, part of which turns to the south as the Somali current near the coast at about 10?N and the rest turns north. At the passage between the African continent and the So- cotra Island, the northern branch separates into two flows: the left one enters the passage and the right one flows eastward along the southern slope of the island. Off the island the flow separates once more, most of it meandering northeast and a small fraction flow- ing southeast. Volume transport calculation suggests that the tidal transport is one or two orders of magnitude smaller than the total transport in this region and it becomes more important near the coast. The average velocity of the flow in the upper layer (0-150 m) is about 20 cm s-1, with a maximum of 53 cm s-1 appearing east of the Socotra Island, and the subsurface layer (200-800 m) has an aver- age velocity of 8.6 cm s-1; the velocity becomes smaller at greater depths. The depth of the seasonal thermocline is about 100 m, above which there is a layer with well mixed temperature and dissolved oxygen. High-salinity and oxygen-rich water appears near the surface of the northern Arabian Sea; a salinity maximum and oxygen minimum at 100 m depth along 8?N testifies the subduction of surface water from the northern Arabian Sea. Waters from the Red Sea and the Persian Gulf also influence the salinity of the area.     

Preliminary Study on Applicability of Microsatellite DNA Primers from Parasite Protozoa Trypanosoma cruzi in Free-living Protozoa

1　Introduction　Generallyknownasacodominantgeneticmarker ,microsatellitehasbeenwidelyusedinstudiesonpopu lationgenetics,high resolutiongenotyping ,genemap ping ,evolution ,linkageanalysis ,conservationbiology ,behaviouralecology ,relationsbetweenparasite…     

VARIATIONAL ITERATION SOLVING METHOD FOR SEA-AIR OSCILLATOR MODEL OF INTERDECADAL CLIMATE FLUCTUATIONS

1IN T R O D U C T IO NInteractionsbetween the ocean and atmosphere con-tributeto climatefluctuationosver a broad spectrum oftime scales.Studiesof those interactionhsave farfo-cused on El Ni o-SouthernOscillation(ENSO)phe-nomenon thathas a period of3to4yea…     

A search for periodicities from a ULX in the LINER galaxy NGC 4736

We report our findings on a new quasi-periodic oscillation (QPO) and a long period from the ultraluminous X-ray source (ULX) X-2 in nearby galaxy NGC 4736 based on the Chandra and XMM-Newton archival data. To examine the timing properties, power density spectra of the source have been obtained using Fast Fourier Transform. Also the spectral parameters of the source have been calculated by obtaining and fitting the energy spectra. Power density spectrum of this source reveals a QPO peak at \(0.73_{-0.14}^{+0.16}~\mathrm{mHz}\) with an fractional rms variability of 16 % using the Chandra data (in the year 2000-lower state of the source). The XMM-Newton data analysis indicates a peak at \(0.53_{-0.35}^{+0.09}~\mathrm{mHz}\) with a fractional rms variation of 5 % (in the year 2006-higher state of the source). These recovered QPOs overlap within errors and may be the same oscillation. In addition, we detect a long periodicity or a QPO in the Chandra data of about (5.2±2.0)×10^?5 Hz (～5.4 h) over 3 σ confidence level. If this is a QPO, it is the lowest QPO detected from a ULX. The mass of the compact object in ULX X-2 is estimated using the Eddington luminosity and a disk blackbody model in the range (10–80) M _⊙.     

A proposal for the theoretical analysis of the interactive coupled effects between urbanization and the eco-environment in mega-urban agglomerations

Mega-urban agglomerations are strategic core areas for national economic development and the main regions of new urbanization. They also have important roles in shifting the global economic center of gravity to China. However, the development of mega-urban agglomerations has triggered the interactive coercion between resources and the eco-environment. The interactive coupled effects between urbanization and the eco-environment in mega-urban agglomerations represent frontier and high-priority research topics in the field of Earth system science over the next decade. In this paper, we carried out systematic theoretical analysis of the interactive coupling mechanisms and coercing effects between urbanization and the eco-environment in mega-urban agglomerations. In detail, we analyzed the nonlinear-coupled relationships and the coupling characteristics between natural and human elements in mega-urban agglomerations. We also investigated the interactive coercion intensities between internal and external elements, and the mechanisms and patterns of local couplings and telecouplings in mega-urban agglomeration systems, which are affected by key internal and external control elements. In addition, we proposed the interactive coupling theory on urbanization and the eco-environment in mega-urban agglomerations. Furthermore, we established a spatiotemporal dynamic coupling model with multi-element, multi-scale, multi-scenario, multi-module and multi-agent integrations, which can be used to develop an intelligent decision support system for sustainable development of mega-urban agglomerations. In general, our research may provide theoretical guidance and method support to solve problems related to mega-urban agglomerations and maintain their sustainable development.     

2008年春季中国南方冰雪冻害林木折损分析

An abrupt ice and snow storm disaster which occurred in the spring of 2008 se-verely destroyed forests over a surprisingly large portion of southern China.A transect crossing Jinggang Mountain-Jitai Basin-Yushan Mountain-Wuyi Mountain was selected as the study area.The authors integrated field data collected in two field surveys to analyze the impacts of the disturbance on forests.The following results were obtained.(1) The extent of damage to plantations along the transect decreased in the order of slash pine > masson pine > mixed plantation > Chinese fir.Slash pine is an introduced species from southern America which is characterized by fast growth,low wood quality and rich oleoresin,and showed a damage rate of 61.3% of samples,of which 70.4% cannot recover naturally.Masson pine is the native pioneer species of forests with harder wood,and 52.5% were damaged due to turpentine,of which 60.9% cannot recovery naturally.Chinese fir is a local tree species and samples showed a rate of 46% and a relative rate of 32.5%,lower than the mixed plantation.(2) From west to east along the transect,we can see that evergreen broad-leaved forest of the western transect on Jinggang Mountain showed the lightest damage extent,and a Cryp-tomeria plantation at an altitude of 700 m was severely destroyed while Chinese fir showed light damage below 700 m and relatively severe damage above 900 m.Masson pine and slash pine in the central transect in Jitai Basin were damaged severely due to turpentine ac-tivities,and closed natural secondary deciduous broad-leaved forest was damaged severely due to high ice and snow accumulation on intertwined shrubs.Masson pine aerial-seeding plantations below 400 m along the eastern transect in Xingguo and Ningdu counties were nearly undamaged for small tree sizes,and Chinese fir at 500-900 m altitude showed a lighter damage extent.However,masson pine which was distributed above 400 m and planted in the 1960s,was severely damaged due to turpentine.     

Modified multispectral space for color representation

IntroductionIn color reproduction and communication, met-amer exists inherently when the conventionalcondition matching used. Therefore , the colordifference introduced makes the accuracy of colorrepresentation be lowered.In order to eli minatemetamer CIE developed the scheme so-calledspectra matching in Publication 15 .2 and speci-fied that using band-pass filters with 5 nminter-val to sample and then reconstruct the originalcolor signal ,thus the color difference will disap-pear[1].Spectra…     

Respiration and Calcification of <Emphasis Type="Italic">Crassostrea gigas</Emphasis>: Contribution of an Intertidal Invasive Species to Coastal Ecosystem CO<Subscript>2</Subscript> Fluxes

Respiration and calcification rates of the Pacific oyster Crassostrea gigas were measured in a laboratory experiment in the air and underwater, accounting for seasonal variations and individual size, to estimate the effects of this exotic species on annual carbon budgets in the Bay of Brest, France. Respiration and calcification rates changed significantly with season and size. Mean underwater respiration rates, deducted from changes in dissolved inorganic carbon (DIC), were 11.4 μmol DIC g⁻¹ ash-free dry weight (AFDW) h⁻¹ (standard deviation (SD), 4.6) and 32.3 μmol DIC g⁻¹ AFDW h⁻¹ (SD 4.1) for adults (80–110 mm shell length) and juveniles (30–60 mm), respectively. The mean daily contribution of C. gigas underwater respiration (with 14 h per day of immersion on average) to DIC averaged over the Bay of Brest population was 7.0 mmol DIC m⁻² day⁻¹ (SD 8.1). Mean aerial CO₂ respiration rate, estimated using an infrared gas analyzer, was 0.7 μmol CO₂ g⁻¹ AFDW h⁻¹ (SD 0.1) for adults and 1.1 μmol CO₂ g⁻¹ AFDW h⁻¹ (SD 0.2) for juveniles, corresponding to a mean daily contribution of 0.4 mmol CO₂ m⁻² day⁻¹ (SD 0.50) averaged over the Bay of Brest population (with 10 h per day of emersion on average). Mean CaCO₃ uptake rates for adults and juveniles were 4.5 μmol CaCO₃ g⁻¹ AFDW h⁻¹ (SD 1.7) and 46.9 μmol CaCO₃ g⁻¹ AFDW h⁻¹ (SD 29.2), respectively. The mean daily contribution of net calcification in the Bay of Brest C. gigas population to CO₂ fluxes during immersion was estimated to be 2.5 mmol CO₂ m⁻² day⁻¹ (SD 2.9). Total carbon release by this C. gigas population was 39 g C m⁻² year⁻¹ and reached 334 g C m⁻² year⁻¹ for densely colonized areas with relative contributions by underwater respiration, net calcification, and aerial respiration of 71%, 25%, and 4%, respectively. These observations emphasize the substantial influence of this invasive species on the carbon cycle, including biogenic carbonate production, in coastal ecosystems.