病毒膜蛋白及其表达和纯化技术的研究进展

病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成，称为膜蛋白，膜蛋白以单聚体，二聚体，三聚体，甚至更多的聚合体的形式存在，具有中和抗体，与靶细胞受体结合，凝集红细胞，诱导细胞融合等多种功能，膜蛋白可采用将其基因整合入载体，并在昆虫细胞，哺乳细胞或细菌中获得体外表达，膜蛋白可在去污剂的作用下形成蛋白质－脂－去污剂的复合物而被溶解，其中去污剂的临界微团浓度，亲水物－亲脂物平衡值，荷电、紫外穿透性等是选择合适去污剂的主要参考因子，溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析，离子交换层析，亲和层析，高效液相层析等技术并结合梯度离心；分级沉淀等进行分离、纯化。     

Effects of sediment properties on surface-generated ambient noise in a shallow ocean

Surface-generalized ambient noise in a shallow ocean waveguide with a sediment layer possessing a specific class of density and sound speed distributions capable of describing a realistic seabed environment is considered in this analysis. This class of non-uniform sediment layer has the density and sound speed distributions varying with respect to depth as a generalized-exponential and an inverse-square function, respectively. The study invokes a formulation developed by Kuperman and Ingenito (Kuperman, W. A., Ingenito, F., 1980. Spatial correlation of surface-generated noise in a stratified ocean. J. Acoust. Soc. Am., 67, 1988-1996.) for surface noise generation, in conjunction with the analytical solutions for the Helmholtz equation corresponding to the sediment layer, to arrive at an analytical expression convenient for numerical implementation. The intensity and spatial correlation of the noise sound field are analyzed with respect to the variation of the system parameters, including frequency, sediment layer thickness, sound speed gradient, with emphasis on the effects of sediment properties on the ambient noise field. The results have demonstrated that the intensity of the noise field is relatively sensitive to the variation of the parameters, but the spatial correlation is affected to a less extent, suggesting that the energy distribution, rather than the spatial structure, of the noise field is more susceptible to the environmental variations.     

中国海洋天然活性物质开发新技术21世纪发展战略

海洋天然活性物质是指海水、沉积物和在海洋环境生存的动植物体中所含有的具有生理活性的物质，一般情况下则指海洋生物体中的生物活性物质。几千年来，人们一直在探索和发现这类物质，并扩大它的应用，在当今人类回归大自然的浪潮中，海洋天然活性物质的开发成了国际热点之一 [1～ 9]。本文在分析了国外的进展，我国的水平与差距后，提出了我国海洋天然活性物质开发新技术的发展战略。     

乙酰褐藻酸丙二酯的制备与表征

为了改变褐藻酸丙二酯的溶解性,本文以氢碘酸为催化剂,将褐藻酸丙二酯与乙酐反应,制备了乙酰褐藻酸丙二酯。生成物不溶于水,可溶于多种中等极性的有机溶剂。本文用IR,~1H-NMR,HGPC对其进行了表征,并对反应条件对产率的影响进行了探讨。     

诊断台风异常路径的三层权重控制点方案

本方案采用850、700、500hPa三个层次的环境平均流场作控制点法。综合考虑这些层次上的引导气流在不同时、空域中的相对重要性。试验结果表明,其效果比单一取某层作引导为佳。且对用来预测台风的未来24小时路径是否异常具有一定的参考价值。     

Neural network for wave forecasting among multi-stations

Unlike in the open sea, the use of wind information for forecasting waves may encounter more ambiguous uncertainties in the coastal or harbor area due to the influence of complicated geometric configurations. Thus this paper attempts to forecast the waves based on learning the characteristics of observed waves, rather than the use of the wind information. This is reported in this paper by the application of the artificial neural network (ANN), in which the back-propagation algorithm is employed in the learning process for obtaining the desired results. This model evaluated the interconnection weights among multi-stations based on the previous short-term data, from which a time series of waves at a station can be generated for forecasting or data supplement based on using the neighbor stations data. Field data are used for testing the applicability of the ANN model. The results show that the ANN model performs well for both wave forecasting and data supplement when using a short-term observed wave data.     

走航测深数据的自动采集

本文介绍应用IBM微机实现走航实时水深及定位数据的自动采集系统。给出了联机系统方框图及采集、传送数据的程序流程图。并对IBM-PC/XT微机与测深仪之间的接口、数码转换和数据处理作了简要的叙述。     

弧后盆地的形成与演化探讨：以东亚陆缘区为例

通过对弧后盆地大地构造体制的讨论，作者认为基属活化作用的产物根据地质，地球物理，地球化学等资料的分析，作者提出结论认为，由于东亚岛弧系岩石圈的均衡作用及海沟外侧冷却大洋岩石圈块体的下沉拖曳牵引等作用，使软流圈在岛弧系下方发生分异，这种分异作用带动东亚陆缘向东扩张，从而产生弧后的张开。     

莫桑比克罗非鱼孵卵期间口腔粘膜变化的研究

用组织学、组织化学和酶技术,对雌性莫桑比克罗非鱼在孵卵前后口腔粘膜的组织结构、抗菌酶的比活力变化进行初步研究。结果表明:在口腔粘膜的上皮中,杯状细胞是一个重要的结构,它随着雌鱼的生理状态的改变而变化。雄鱼、雌鱼和孵卵期的雌鱼口腔粘膜上皮中的杯状细胞数量上的差别显著。孵卵期的雌鱼口腔粘膜类似于哺乳动物的子宫粘膜也是受性激素影响的靶器官。该种鱼的口腔粘膜中存在着两种抗菌酶;蛋白质水解酶和壳多糖酶。孵卵前后,这两种酶的比活力分别提高1.4—2.03倍。说明孵卵时,口腔粘膜为使卵及仔鱼健康的发育,增强了抗菌能力。研究结果证明:抗菌力的增加与口腔粘膜中的杯状细胞的大量增生有着密切的关系。     

潮汐对瞬态气候响应模拟的影响研究

In this study, the impact of oceanic processes on the sensitivity of transient climate change is investigated using two sets of coupled experiments with and without tidal forcing, which are termed Exp_Tide and Exp_Control,respectively. After introducing tidal forcing, the transient climate response(TCR) decreases from 2.32 K to 1.90 K,and the surface air temperature warming at high latitudes decreases by 29%. Large ocean heat uptake efficiency and heat storage can explain the low TCR in Exp_Tide. Approximately 21% more heat is stored in the ocean in Exp_Tide(1.10×10~(24) J) than in Exp_Control(0.91×10~(24) J). Most of the large ocean warming occurs in the upper 1 000 m between 60°S and 60°N, primarily in the Atlantic and Southern Oceans. This ocean warming is closely related to the Atlantic Meridional Overturning Circulation(AMOC). The initial transport at mid-and high latitudes and the decline in the AMOC observed in Exp_Tide are both larger than those observed in Exp_Control. The spatial structures of AMOC are also different with and without tidal forcing in present experiments. The AMOC in Exp_Tide has a large northward extension. We also investigated the relationship between AMOC and TCR suggested by previous studies using the present experiments.