大黄鱼倍性检测研究

于2001—2004年对人工诱导大黄鱼的不同发育阶段和不同组织进行倍性检测，胚胎眼泡期采用染色体计数法测定，鱼苗和成鱼阶段用流式细胞法，结果表明，胚胎染色体数目2n=48条为二倍体，3n=72条为三倍体．采用流式细胞法测定鱼苗和成鱼个体时，若受测样品的DNA相对含量值与已知对照组二倍体的比值约为1．5：1，其个体为三倍体；比值约为1：1，则为二倍体，无论用肌肉或尾鳍组织，皆可检测出其个体的倍性。     

A Computational Model for Velocity Separation in Shallow Sea

Based on the hydrodynamical feature and the theoretical velocity profiles of tidal flow and vvind-induced flow in shal-low sea, a computational model is established for the first time, which can separate observed velocity into tidal velocity and wind-induced velocity by use of the least square method. With the model, not only the surface velocities of tidal flow and vvind-induced flow are obtained, but also the bed roughness height is determined and the wind velocity above the wa-ter surface is estimated. For verification of the model, the observed velocity in the Yellow River Estuary and the laborato-ry test is separated, then it is applied to the Yangtze River Estuary. All the results are satisfactory. The research results show that the model is simple in method, feasible in process and reasonable in result. The model is a valid approach to analysis and computation of field dala, and can be applied to separate the observed velocity in shallow sea; at the same time, reasonable boundary conditions of th     

三疣梭子蟹全人工工厂化育苗技术

2000年笔者在日照市水产研究所利用1450m^3水进行梭子蟹全人工工厂化育苗生产，共出Ⅱ-Ⅲ期幼蟹512．7万只，创造了出苗量为3500只／m^3，总产值为150多万元的直接经济效益，同时对幼体培育的密度进行了初步试验，结果表明：从Z1至M期幼体培育密度10－15万／m^3为宜，M期以后密度以0．8－1．5万／m^3为宜。     

新建水坝对下游已建水工建筑物的影响分析

分析了新建水坝及社会经济发展因素对坝下游水流及泥沙的影响，用一个简易河床模型来说明新建大坝后下游河床的演变规律，通过坝下游河床一般演变分析来阐述局部冲刷对已建水工建筑物基础的影响及对已建水工建筑基础的防护措施。     

病毒膜蛋白及其表达和纯化技术的研究进展

病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成，称为膜蛋白，膜蛋白以单聚体，二聚体，三聚体，甚至更多的聚合体的形式存在，具有中和抗体，与靶细胞受体结合，凝集红细胞，诱导细胞融合等多种功能，膜蛋白可采用将其基因整合入载体，并在昆虫细胞，哺乳细胞或细菌中获得体外表达，膜蛋白可在去污剂的作用下形成蛋白质－脂－去污剂的复合物而被溶解，其中去污剂的临界微团浓度，亲水物－亲脂物平衡值，荷电、紫外穿透性等是选择合适去污剂的主要参考因子，溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析，离子交换层析，亲和层析，高效液相层析等技术并结合梯度离心；分级沉淀等进行分离、纯化。     

Rain-Flow and Reverse Rain-Flow Counting Method for the Compilation of Fatigue Load Spectrum

The rain-flow counting method is widely used to compile the fatigue load spectrum. The second stage counting of the rain-flow method is a troublesome process. In order to overcome this drawback, the rain-flow and reverse rain-flow counting method is proposed in this paper. In this counting method, the rule for counting of the rain-flow method is modified, so that the sequence of load-time need not be adjusted. This is a valid and useful method to count cycles and compile the load spectrum and it can be widely used in ocean engineering.     

培养基对海藻真菌PT2菌株的生长及活性代谢产物合成的影响

本文采用PD、GPY、YES、SYE、GPM5种不同培养基培养海藻真菌菌株PT2，并对其在不同培养基中的生长情况、代谢产物的形成及抗肿瘤活性进行分析，旨在了解不同培养基对菌株生长和活性物质合成的影响．结果显示，PT2菌株在不同培养基上的培养特征及生长速率有所不同，液体培养基发酵液抽提物的抗肿瘤活性也有较大差别，其中SYE发酵液抽提物活性最强，对KB、Raji和Hela 3种肿瘤细胞的IC50分别为7．6μg／cm^3、2．4μg／cm^3和3．3μg／cm^3．采用HPLC法对不同抽提物进行分析，发现它们之间的组成成分有显著差异．本文的实验结果表明，不同培养基对该菌株的生长及活性代谢产物的合成具有显著的影响。     

现场海水中有机污染物光学测量方法研究

介绍了一种用光学方法对海水中的有机污染物（主要包括：叶绿素-a、黄色物质、石油）进行测量的光学系统，该系统可同时测量海水中多种有机污染物的种类和浓度。     

苏北盐城凹陷复合含油气系统

苏北盐城凹陷含油气系统可划分为上、下2个复合含油气系统。上复合含油气系统，即Pz、K2p、K2t(生)-K2t、E1f1(储)一E1f1 E1f2(盖)，古生界、中生界浦口组和泰州组为烃源岩层，泰州组和阜宁组一段为储集层，阜宁组一段顶部至二段暗色泥岩为区域盖层，油气沿深大断裂运移，油气成藏关键时刻在古近纪晚期至新近纪早期，有利勘探区在深大断裂附近的中、新生界发育地区；下复合含油气系统，即Pz、K2p(生)-Pz、K2p(储)-K2p3 K2p4(盖)，油气通过断层或不整合运移，油气大量生成的关键时刻为古近纪早期，该系统中气藏分布在古生界被中生界覆盖地区。     

Metabolites from mangrove endophytic fungus Dothiorella sp.

Mangroves are special woody plant communities in the intertidal zone of tropical and subtropical coasts. They prove to be a natural microorganisms and new metabolites storage. In the study of mangrove endophytic fungi metabolites, four new compounds, Compounds 1, 2, 3 and 4, as well as a known octaketide, cytosporone B (5), are isolated from an endophytic fungus, Dothiorella sp., HTF3. They all show cytotoxic activities. The elucidation of these structures is mainly based on 1D/2D NMR and ESI-MS spectral analyses.