Gaussian decomposition and component pigment spectral analysis of phytoplankton absorption spectra

The absorption spectrum of phytoplankton is an important bio-optical parameter for ocean color hyperspectral remote sensing;its magnitude and shape can be affected considerably by pigment composition and concentration.We conducted Gaussian decomposition to the absorption spectra of phytoplankton pigment and studied the spectral components of the phytoplankton,in which the package effect was investigated using pigment concentration data and phytoplankton absorption spectra.The decomposition results were compared with the corresponding concentrations of the five main pigment groups(chlorophylls a,b,and c,photo-synthetic carotenoids(PSC),and photo-protective carotenoids(PPC)).The results indicate that the majority of residual errors in the Gaussian decomposition are0.001 m~(-1),and R~2 of the power regression between characteristic bands and HPLC pigment concentrations(except for chlorophyll b)was0.65 or greater for surface water samples at autumn cruise.In addition,we determined a strong predictive capability for chlorophylls a,c,PPC,and PSC.We also tested the estimation of pigment concentrations from the empirical specific absorption coefficient of pigment composition.The empirical decomposition showed that the Ficek model was the closest to the original spectra with the smallest residual errors.The pigment decomposition results and HPLC measurements of pigment concentration are in a high consistency as the scatter plots are distributed largely near the 1:1 line in spite of prominent seasonal variations.The Wozniak model showed a better fit than the Ficek model for Chla,and the median relative error was small.The pigment component information estimated from the phytoplankton absorption spectra can help better remote sensing of hyperspectral ocean color that related to the changes in phytoplankton communities and varieties.     

Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone,China

Journal of Oceanology and Limnology - Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological significance in Shandong Province, China. Although B....     

Farmer-based Rice Cropping Systems in the Poyang Lake Region,China: Analysis of Characteristics and Policy Implications

This paper examines the spatial characteristics of farmer/household behaviors in regional rice cropping systems (RCS), and the results provide necessary information for developing strategies that will maintain regional food security. Through field study and statistical analysis based on 402 households questionnaires finished in 2014-2015 in the Poyang Lake Region (PLR) of China, we arrived at two main conclusions. First, single- and double cropping rice were found across the study area, but showed a general distribution trend, with double cropping rice in the southeast part (especially in Jinxian county) and single cropping rice in the northwest (particularly in De’an county). Second, the household decisions concerning RCS varied in different parts of the PLR, but double cropping was the dominant type, with about 63.57% of the respondent households in the PLR cultivating double cropping rice. However, the multiple-cropping index of paddy rice was only 1.55. About 3% of interviewed households had altered their RCS during this period. Based on these findings, the local governments should guide farmers’ paddy field cultivation behaviors by increasing the comparative efficiency of rice production, promoting appropriate scale operations and land conversion, as well as optimizing rice growing conditions to improve the multiple cropping index and enhance food provision. Finally, land-use efficiency and more sustainable use of land resources should be improved.     

一例异育银鲫(Carassius auratus gibelio)暴发性出血病病原分析

2016年6月,江苏某异育银鲫(Carassius auratus gibelio)养殖场暴发一种传染性急性出血病,造成养殖银鲫大量死亡。为分析此次疾病病因及流行规律,本研究从发病养殖场采集患出血病的异育银鲫,从细菌、病毒及寄生虫三个方面对病原进行了分析。采用病原菌分离、组织病理学观察、超薄切片电镜观察、病毒核酸分析、回感实验等对病原进行鉴定。结果显示从发病鲫鱼体内分离到病毒一株,未发现寄生虫及细菌感染。经测序及序列分析,该病毒为鲤疱疹病毒Ⅱ型(Cyprinid herpesvirus2,CyHV-2)病毒,组织病理学观察结果显示患病鱼的鳃和肾脏有明显病变,电镜下可观察到病鱼脾脏组织有带囊膜的球形病毒,囊膜直径约为170—200nm,病毒衣壳直径约为110—120nm,核心直径约为60nm,用组织匀浆感染鲫鱼囊胚细胞系(CGB)可稳定地观察到典型的细胞病变,用患病鱼组织匀浆液人工感染异育银鲫的死亡率高达100%,荧光定量PCR检测到该病毒可感染多器官,其中以脾脏中病毒含量最高,其次是脑,肝脏中最少。本研究可为CyHV-2的诊断防控及疫苗研制提供资料。     

氨氮急性攻毒对水产经济动物棘胸蛙(Paa spinosa)蝌蚪死亡率、排氨率、耗氧率及窒息点的影响

以体长(1.932±0.204)cm、体质量(1.386±0.055)g的棘胸蛙蝌蚪为实验动物,在水温(24±0.2)℃、DO(7.30±0.01)mg/L、pH 7.30±0.01条件下,采用静水停食法开展了氨氮对蝌蚪的急性攻毒实验,并以此为基础,测定了不同氨氮质量浓度胁迫下棘胸蛙蝌蚪的排氨率、耗氧率、氧氮比及窒息点。结果表明:(1)蝌蚪对氨氮急性攻毒具明显的运动避毒行为,濒死个体的背部皮肤和肝脏均具明显的色变症状;(2)氨氮对蝌蚪的急性致死率具明显的剂量—时间效应,24h、48h、72h、96h LC50依次为177.5、151.7、148.6和146.8mg/L,毒性时段蓄积程度系数呈持续下降趋势,24—48h、48—72h、72—96h的时段MAC值分别为84.04%、10.1%和5.86%;(3)蝌蚪夜均、昼均、日均及时段排氨率随氨氮质量浓度增加均呈阶梯式下降趋势,其中时段排氨率与对照组均无显著差异的为9.80mg/L实验组,夜均、昼均、日均及时段排氨率与对照组均无显著差异的仅为2.45mg/L实验组(P0.05);(4)氨氮对蝌蚪呼吸耗氧具低毒兴奋效应,其夜均、昼均和日均耗氧率随氨氮质量浓度增加均呈先升后降趋势,峰值氨氮质量浓度范围均为4.90—7.35mg/L(P0.05),与对照组均无显著差异的仅为14.70mg/L实验组(P0.05),窒息点含氧量随氨氮质量浓度增加呈先降后升趋势,谷底出现于4.90mg/L实验组(P0.05),与对照组无显著差异的仅为9.80mg/L实验组(P0.05);(5)实验期间,蝌蚪氧氮比(O:N)波动于18.87—25.34之间,昼均和日均氧氮比(O:N)随氨氮质量浓度增加均依次呈"上升—稳定—下降—再稳定"之趋势,两者的峰值氨氮质量浓度范围分别为4.90—14.70mg/L和4.90—9.80mg/L,夜均氧氮比(O:N)呈先升后降趋势,峰值出现于7.35mg/L实验组(P0.05)。     

基于F型mtDNA D-Loop的厚壳贻贝(Mytilus coruscus)群体遗传多样性研究

为研究东海区厚壳贻贝(Mytilus coruscus)遗传多样性,以厚壳贻贝F mtDNA D-loop为标记,对浙江省舟山嵊山岛、宁波渔山岛、温州南麂岛、福建省宁德湾和莆田南日岛五个海区的厚壳贻贝群体进行了遗传分析。结果表明,厚壳贻贝各群体的遗传多样性差异不明显,在5个群体中,宁德群体的遗传多样性相对最丰富;将5个群体作为一个整体时,呈现出较高的单倍型多样性和较低的核苷酸多样性。对厚壳贻贝5个群体间的遗传分化系数(Fst)和基因流(Nm)进行检测,结果显示群体间Fst值都很低,但Nm值都很高(Nm绝对值1),表明5个群体间存在丰富的基因交流。但宁德群体与浙江沿海的3个群体(嵊山、渔山、温州)的Fst值相对较高,且差异显著(P0.5),表明宁德群体与这3个群体间出现遗传分化。本研究旨在为海洋经济贝类资源的保护管理提供理论依据。     

基于耀斑改正的多光谱水深反演方法研究

利用WorldView-2四波段卫星数据和电子海图数据,基于改进的耀斑改正算法和双波段比值算法,反演获得了3处典型海域的浅水水深。通过不同海域、不同耀斑条件下水深反演实验,探讨了RED,NIR波段在典型四波段水深反演中的作用和影响,发现在双波段比值法水深反演中,引入RED+NIR波段进行耀斑改正处理,可以增加珊瑚、海藻等绿色物质覆盖海底的反射率,有效地提高该类海域的水深反演精度。基于耀斑改正的多光谱水深反演方法,适用于中轻度耀斑条件下,水质较清澈的浅海水深反演,可在国内外典型四波段卫星数据水深反演中推广应用。     

一种新型鲸鱼式潮流能发电装置的设计与试验研究

为了进一步提高潮流能的利用率,提出了一种新型鲸鱼式潮流能发电装置。借鉴风机叶片设计方法及水平轴水轮机的设计原理,利用结构力学、流体力学和CFD相关方法,对该装置的螺旋桨叶、导流筒和固定桩等部件进行了设计研究,从获能效果、装置可靠性和发电功率等方面进行了优化设计,并通过900 W样机试验验证了设计的有效性。试验结果显示,海流高潮期装置最大发电功率可达到980 W,一天内大约有4个缓潮期,此间发电功率明显下降,但持续时间不长,总体平均发电功率在800 W上下;同时潮流能轮机启动流速在0.41 m/s左右,有着良好的低速启动性能。     

滩涂机载LiDAR点云数据滤波方法研究

机载LiDAR点云数据滤波是制作高精度数字地形产品的重要环节。通过分析常用滤波算法基本原理、滩涂机载LiDAR点云数据的特点,提出了一种基于潮汐水位高程约束,移动窗口滤波与三角网渐进加密滤波组合的滤波方法。试验表明:该滤波方法获取的滩涂地形有效点云数据质量与人工滤波结果相当,在一定程度上提高了滩涂区域机载LiDAR点云数据滤波的效率,为快速获取滩涂区域数字高程模型提供了有效途径。     

顾及局部与结构特征的稀疏多项式逻辑回归高光谱图像分类方法

稀疏多项式逻辑回归在分类中仅利用图像光谱信息,导致分类效果不太理想.本文提出了一种顾及局部与结构特征的稀疏多项式逻辑回归高光谱图像分类方法.首先利用加权均值滤波与拓展形态学多属性剖面对原始高光谱图像进行局部与结构特征提取;然后对二者进行加权平均特征级融合以获取更具唯一性的像元特征;最后由稀疏多项式逻辑回归分类器对融合结果进行分类.结果表明,本文方法能有效地提高分类精度,而且具有较强的稳健性.