A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.     

基于RS与GIS的北京市热岛研究

基于普适性单通道算法,利用2005年5月6日的TM数据反演北京市区的实际地表温度。在此基础上,分析了北京市区的热环境特征、热岛分布的位置。运用GIS空间分析工具,分析了地表温度与地形特征和地表覆盖类型之间的关系。结果表明,北京市热岛分布具有环状和带状分布特征,高程较低处和坡度较小处更容易形成热岛中心,不同地表覆盖类型的热特征具有显著的差异。     

创伤弧菌和哈氏弧菌双重PCR检测方法的建立及初步应用

根据创伤弧菌(Vibrio vulnificus)的溶细胞毒素基因序列和哈氏弧菌(Vibrio harveyi)的toxR基因序列,分别设计并合成两对特异性引物,通过PCR反应条件优化,测试两种菌的特异性和敏感性,建立双重PCR方法,同时快速检测V.vulnificus和V.harveyi。结果表明:纯培养V.vulnificus和V.harveyi的检测灵敏度分别是12 cfu/mL和18 cfu/mL,与无乳链球菌、海豚链球菌、副溶血弧菌及美人发光杆菌无交叉反应;此PCR检测方法具有良好的特异性、敏感性,具快速、高效等优点,对细菌V.vulnificus和V.harveyi诊断与防治具有较好的临床应用性。     

我国硅灰石矿产资源及勘查开发现状和发展建议

我国硅灰石矿资源储量居世界第一,且产量居世界前列。随着多年的勘查与开发,硅灰石矿的开发利用已由传统领域向新的应用领域探索拓展。本文在收集整理现有硅灰石矿床、海关进出口等数据资料的基础上,在全国范围内搜集了77个矿产地,其中超大型8处,划分了7个重要成矿区,总结了我国硅灰石矿产开发利用现状、进出口贸易情况,对硅灰石矿产的行业发展提出了建议。     

经典平差模型的扩展

将经典测量平差的概括模型(附有等式限制条件的条件平差模型)扩展为附不等式约束的平差模型,同时将其定义为测量平差统一模型,并在一定的条件下实现这种平差模型与概括平差模型间的相互转换.在最小二乘平差原则下,作者结合虚拟观测方程处理不等式约束的思路,给出附不等式约束平差的最小二乘显式解,分析其统计性质,并修正传统测量平差结果的统一表达式.按本文所提方法,附不等式约束的平差问题,完全可以利用传统的平差方法和平差程序求解,方法简单可行.     

基于GIS的城市工程建设多测合一管理平台设计与实现

近年来,为了加快疏解企业办事堵点、痛点,深化"放管服"改革,优化营商环境建设,最大限度惠企便民,国内许多城市开始深化工程建设项目审批制度改革,全面开展工程建设项目多测合并、联合测绘和成果共享工作.结合东莞市工程建设项目审批的实际情况,探讨多部门协同的多测合一(联合测绘)管理流程,构建多测合一全过程管理数据库,开发东莞市...     

基于分数阶时间导数的黏弹性衰减VTI介质中平面波传播

目前在地震勘探频带范围内通常假设品质因子Q与频率无关,且呈衰减各向同性.事实上,相比较速度各向异性,介质的衰减各向异性同样不可忽视.本文将衰减各向异性和速度各向异性二者与常Q模型相结合,建立了黏弹性衰减VTI介质模型,并基于分数阶时间导数理论,给出了对应的本构关系和波动方程.利用均匀平面波分析和Poynting定理,推...     

Carbon isotope evidence of last glacial climate variations in the tropical NW Leizhou Peninsula,South China

Zhong, W., Cao, J., Xue, J., Ouyang, J., Wang, H., Tang, X. & Gao, X. 2012 (January): Carbon isotope evidence of last glacial climate variations in the tropical NW Leizhou Peninsula, South China. Boreas, Vol. 41, pp. 102–112. 10.1111/j.1502‐3885.2011.00224.x. ISSN 0300‐9483. Bulk organic δ¹³C values of sediment sequences are commonly used to indicate past climatic changes, although the causes and mechanisms leading to the observed organic carbon isotope responses are presently not fully understood. Based on two sedimentary sequences, namely a peat profile and a fluvial and swamp sequence from the tropical NW Leizhou Peninsula in South China, we interpret more negative bulk organic δ¹³C values to suggest wetter and warmer conditions, implying a stronger East Asian (EA) summer monsoon, and less negative δ¹³C values to indicate drier and cooler conditions, reflecting a weakening of the EA summer monsoon. A warm and wet period occurred between c. 48.0 and 28.0 cal. ka BP. In the study region, a climate shift occurred at c. 22.0 (or 20.0) cal. ka BP, and the driest and coldest period occurred between c. 19.0 and 16.0 cal. ka BP. After c. 12.0 cal. ka BP, the climate changed towards wetter and warmer conditions. Several possible millennial‐scale dry and cold oscillations characterized possibly by C4 plants, or by more positive organic δ¹³C values during the period when C3 plants dominated, are cautiously and tentatively interpreted as showing a similarity with Heinrich events and other cold millennial‐scale oscillations evidenced from a Greenland ice core and Chinese stalagmite. We demonstrate that bulk organic δ¹³C records of sedimentary sequences in the study region have potential for indicating the changes in vegetation composition that were closely associated with climate variation during the last glacial period.     

The rule of release of carbon and heavy metals during weathering of rocks—A preliminary study

Release of carbon from rocks during their weathering is closely linked to its global circulation. The contents and chemical forms of inorganic carbon in limestone, mudstone, granite and basalt as well as in their weathering and pedogenic products have been examined. The results show that a geochemical process of carbon release from the rocks in the early stage of weathering is followed by its accumulation in the subsequent weathering products. The environmental geochemical behavior of some heavy metals related to inorganic carbon release and its accumulation are also studied in this paper. Experimental results show that the capacity of manganese to associate with carbonates increases with increasing concentrations of the latter, but the behavior of copper is opposite to that of manganese. Translated by Chen Mingzhong, Chengdu Library, Academia Sinica.     

利用卫星测高资料反演海底地形研究

论述了利用卫星测高数据反演海底地形地解析算法和统计算法的基本原理和数学模型，在此基础上，基于最小二乘配置理论，提出了统计算法的改进模型。使用新模型在南中国海地区进行了海底地形反演计算，并将反演结果与实际船测水深进行比对，进一步验证改进模型的可靠性和有效性。