性类固醇激素及其受体在半滑舌鳎性腺分化发育过程中的表达与生理功能

首次用17β-雌二醇(E_2)及其受体(ERα)、睾酮受体(AR)的多克隆抗体在半滑舌鳎(Cynoglossus semilaevisG(u|¨)nther)性腺分化发育过程中进行免疫细胞化学定位研究。结果表明:在半滑舌鳎原始性腺中已有E_2、ERα和AR的分布和表达;卵原细胞、初级卵母细胞、精原细胞、初级精母细胞、次级精母细胞对E_2及ERα、AR抗体均呈现不同程度的阳性反应,在精细胞和精子中对E_2和ERα呈免疫阴性反应,而AR则一直保持较强表达,揭示了半滑舌鳎性腺中类固醇激素可能通过旁分泌调控机制刺激卵子和精子发育和成熟。性类固醇激素及其受体免疫阳性物定位在生殖细胞的胞质、胞膜和核膜等广泛部位并有表达,在性腺分化和不同发育时期的表达强度有所不同,表明其在性腺分化发育过程各阶段所起生理功能不同。这些结果为证明性类固醇激素及受体参与调节半滑舌鳎性腺分化发育提供了重要形态学新证据。     

A preliminary study of variations of the Changjiang Diluted Water between August of 1999 and 2006

A large area hypoxia has been already reported respectively by two interdisciplinary surveys off the Changjiang Estuary since summer of 1999 and 2006. The hypoxic zone shows distinct year-to-year variations. Observed oceanographic data are first analysized and reveal a big difference for the Changjiang Diluted Water (CDW) between these two periods. These great changes are related to the tremendous reduction of the freshwater discharge and variations of wind fields between these two years. It is also found that the monthly mean intrusion of Kuroshio and its branches has increased in the northern East China Sea (ECS), but decreased in the southern ECS in August of 2006 as compared with 1999 on the base of general circulation models. Then, the Regional Ocean Modelling Systems is applied to the East China Sea to evaluate the contributions and relative importance of impacts from the river discharge, wind forcing and open boundary data. Our simulations reproduce the phenomena that more fresh water extends northeastward in 2006 and forms a negative SSS anomaly to the northeast of the river mouth as compared with 1999, which is consistent with observations. The five group numerical tests suggest that the wind forcing dominates the CDW variations followed by the Kuroshio and its branches. The study implies important roles played by hydrodynamic processes on the variability of hypoxic zone in the study areas.     

牙鲆受精的细胞学研究

通过组织切片,对牙鲆(Paralichthys olivaceus)的受精过程进行细胞学观察发现,这一过程包括精子入卵、卵母细胞第二次成熟分裂排出第二极体、雌雄原核形成与融合,直到合子进行第一次有丝分裂,在受精的整个过程中所有观察过的受精卵都只发现一个精子形成原核,并最终与卵原核结合形成合子,由此判断牙鲆的受精方式是单精受精.本实验的研究结果,不仅能够从理论上丰富海水鱼类的受精生物学基础研究资料,又可为生产中牙鲆染色体操作遗传育种提供依据.     

Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder Sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes,12 confirmed hybridization of the paternal and matemal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.     

达州市气候变化的特征分析

基于达县测站1961-2007年的地面常规观测资料,主要采用小波分析,结合趋势分析、功率谱分析等方法分析了达州市47年来近地表气温、降水随时间的变化特征.结果表明,达州市近50年来冬季(年平均)地表气温变暖幅度约为0.9℃(0.4℃),增温速率接近0.18℃/10a(0.08℃/10a),低于全球的升温幅度.利用小波分析方法研究冬季气温的周期性发现,达州市的冬季气温存在着明显的8年和50年以上的特征时间尺度和周期性振荡.通过对降水的趋势分析发现,夏季降水的增加幅度比年均显著.小波分析方法结果显示达州市的夏季降水同样存在50年以上的特征时间尺度,但在小尺度上20世纪70年代末前后存在不同的变化特征.     

水资源开发利用引起的环境负效应——以玛纳斯河流域为例

通过对玛纳斯河流域环境负效应研究,确定了水资源开发利用过程中引起环境负效应的制约因子,引入地下水动态均衡方法进行半定量分析,明确地下水量的变化为引起环境负效应的直接因素,为定量进行水资源综合开发与利用、防止环境负效应的产生提供方法和依据。     

基于PALSAR数据的DEM提取方法研究

针对PALSAR Level 1.1数据,研究使用NASA/JPL提供的开源干涉软件包ROI_PAC Version 3.0提取DEM.ROI_PAC的目前版本只能处理Level 1.0数据,因此,文章在分析了ROI_PAC软件包处理流程的基础上,提出处理Level 1.1数据的方法,并用PALSAR Level 1.1数据对该方法做了验证.干涉重建DEM与参考DEM的对比结果表明,二者的差异均值为0.27 m,标准差为±9.24 m,80%像元点的高程误差在±10 m以内.     

矢量图形与影像图像一体化实现

本文在阐述影像金字塔相关理论（构建方法、影像切割等）的基础上,采用VC2005编程实现了在同一GIS系统中进行矢量数据和栅格数据叠加显示的功能模块。该模块可确定是否加载影像,并根据当前矢量图显示范围自动加载不同分辨率的影像图。     

基于线段的基本线/线拓扑关系计算

空间拓扑关系是空间数据建模、空间查询、分析、推理、制图综合、数据质量控制、数据更新等的基础。目前空间拓扑关系研究的主要成果仍然集中在拓扑关系的描述方面,在计算方面的阐述都非常薄弱,不足以指导系统开发人员编程实现。本文研究了IR2中两个简单线目标间拓扑关系的计算方法,重点分析了线/线拓扑关系计算的特点,提出了一种基于线段的线/线拓扑关系计算方法,并用VC＋＋采用底层开发模式实现了基本线/线拓扑关系的计算。     

ETM遥感图像在措勤东部第四系含水性研究中的应用

措勤东部属于极端缺水的地区,利用遥感技术开展第四系松散堆积物含水性调查具有重要作用。本文选用ETM753与8波段融合图像作为遥感解译底图,结合地形地貌、地质和气象影响,建立残积、冲积、洪积、冲洪积、冰川永久积雪、湖积、沼泽和坡积的识别知识体系,划定第四系松散堆积物的高、中、低、差四个含水等级,利用G IS实现第四系松散堆积物及其含水性的高程、坡度和坡向分布,为区内水资源的深入调查提供有力支持。