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71.
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Phytoplankton growth rates and mortality rates were experimentally examined at 21 stations during the 2017 spring intermonsoon(April to early May) in the northern and central South China Sea(SCS) using the dilution technique, with emphasis on a comparison between the northern and central SCS areas which had different environmental factors. There had been higher temperature but lower nutrients and chlorophyll a concentrations in the central SCS than those in the northern SCS. The mean rates of phytoplankton growth(μ_0) and microzooplankton grazing(m) were(0.88±0.33) d~(–1) and(0.55±0.22) d~(–1) in the central SCS, and both higher than those in the northern SCS with the values of μ_0((0.81±0.16) d~(–1)) and m((0.30±0.09) d~(–1)), respectively.Phytoplankton growth and microzooplankton grazing rates were significantly coupled in both areas. The microzooplankton grazing impact(m/μ_0) on phytoplankton was also higher in the central SCS(0.63±0.12) than that in the northern SCS(0.37±0.06). The microzooplankton abundance was significantly correlated with temperature in the surface. Temperature might more effectively promote the microzooplankton grazing rate than phytoplankton growth rate, which might contribute to higher m and m/μ_0 in the central SCS. Compared with temperature, nutrients mainly affected the growth rate of phytoplankton. In the nutrient enrichment treatment,the phytoplankton growth rate(μn) was higher than μ_0 in the central SCS, suggesting phytoplankton growth in the central SCS was nutrient limited. The ratio of μ_0/μn was significantly correlated with nutrients concentrations in the both areas, indicating the limitation of nutrients was related to the concentrations of background nutrients in the study stations. 相似文献
73.
中国卤虫早期胚胎发育相关基因的研究--mRNA差异显示技术的三种不同染色方法比较 总被引:1,自引:0,他引:1
利用mRNA差异显示技术研究中国孤雌生殖卤虫胚胎发育的相关基因,并对中国孤雌生殖卤虫差异显示中SYBR Green Ⅰ、硝酸银和溴化乙锭三种染色方法进行了比较。以三种单碱基锚定引物对发育0h,4h,8h,12h,16h和10天的中国孤雌生殖卤虫的RNA进行逆转录和26种随机引物进行PCR反应,经非变性的6%聚丙稀酰胺凝胶电泳后,分别用SYBR Green Ⅰ、硝酸银和溴化乙锭染色,最后进行差异条带的回收和再扩增。结果表明:(1)6种样本的DD-PCR产物显示长200-3.0k不等的扩增片段,条带清晰,并呈现出有无和强弱的变化(2)SYBR Green Ⅰ和硝酸银染色的灵敏度比溴化乙锭染色的灵敏度高(3)经SYBR Green Ⅰ和溴化乙锭染色的凝胶的回收和再扩增效率比硝酸银染色的高(4)水煮法和NAP-25过柱法对凝胶片段的回收效果几乎相当。利用mRNA差异显示技术研究中国孤雌生殖卤虫胚胎发育相关基因时,得到了较好的扩增片段;三种染色方法中SYBR Green Ⅰ染色灵敏度较高,并且SYBR Green Ⅰ染色有利于凝胶片段的回收和再扩增。 相似文献
74.
马尾藻类是重要的经济海藻。自古以来,我国劳动人民就以马尾藻作为药材和食品;近几年来,随着医药工业的发展,马尾藻类不仅越来越广泛地用在医药上,而且成为提取褐藻胶、甘露醇、碘和氯化钾的重要工业原料之一。因此,弄清这类海藻的种类、分布和资源情况是有效地开发、利用马尾藻类海藻的前提。西沙群岛地处我国海防前哨,海洋植物资源很丰富,马尾藻类种类也较多,为了更好地开发利用西沙群岛的海藻资源,使其为祖国的社会主义建设和工、农、兵服务,中国科学院海洋研究所曾于1957年,1958年,特别是无产阶级文化大革命后的1975年,1976年前后四次对西沙群岛的海藻进行了调査,收集了大量的标本。在此基础上,我们对西沙群岛的马尾藻科海藻进行了较为系统的研究,本文仅是这项研究任务的一部分,以后将陆续整理发表。 相似文献
75.
冲绳海槽的形成与发展 总被引:1,自引:0,他引:1
冲绳海槽是位于东海大陆架外缘、东海陆架边缘隆褶带与琉球岛弧之间的一个狭长带状弧间盆地.关于它的成因,已有古琉球弧直接张裂和断裂下陷两种观点,而笔者认为,它是由于菲律宾海板块从早中新世末以来,向欧亚大陆板块下俯冲所形成的沟-弧-盆系列的一个组成部分,在其生成和发展过程中,先后经历了拗陷、断陷和扩张三个阶段.然而,扩张刚刚开始.今后它将进一步裂开,琉球弧将更加向菲律宾海方向推进.因受调查程度的限制,本文将以冲绳海槽北半部的资料为主要依据. 相似文献
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77.
植物硅酸体的研究及在我国第四纪地质学中的初步应用 总被引:8,自引:0,他引:8
本文简述了国外植物硅酸体(植物蛋白石)这门新兴学科的进展情况,介绍了植物硅酸体研究在我国第四纪地质学中如古气候与古环境、地层时代、考古等方面的初步应用。 相似文献
78.
Ji-ning Song Lin Lu Bin Teng Han-il Park Guo-qiang Tang Hao Wu 《Ocean Engineering》2011,38(11-12):1308-1322
Laboratory tests have been conducted on vortex-induced vibration (VIV) of a long flexible riser towed horizontally in a wave basin. The riser model has an external diameter of 16 mm and a total length of 28.0 m giving an aspect ratio of about 1750. Reynolds numbers ranged from about 3000 to 10,000. Fiber optic grating strain gages are adopted to measure the dynamic response in both cross-flow and in-line directions. The cross-flow vibrations were observed to vibrate at modes up to 6 and the in-line reached up to 12. The fundamental response frequencies can be predicted by a Strouhal number of about 0.18. Multi-mode responses and the asymmetry of the bare pipe response in uniform flow were observed and analyzed. The experimental results confirmed that the riser pipe vibrated multi-modally despite it being subject to a uniform current profile and all of the excited modes vibrated at the Strouhal frequency. The asymmetrical distribution of displacement mainly resulted from the modal composition. Higher harmonics of the VIV response such as the third, fourth and fifth harmonics frequencies were found to be steady over the entire duration of the test even if they varied along the length of the riser pipe. 相似文献
79.
In this study, on the basis of the Floquet transform method, a numerical model for the simulation of the vibration isolation via multiple periodic pile rows with infinite number of piles is established. By means of the fictitious pile method due to Muki and Sternberg, the second kind of Fredholm integral equations for the pile rows are developed by using the fundamental solutions for the half‐space and the compatibility conditions between the piles and half‐space. Employing the Floquet transform method, integral equations for the pile rows in the wavenumber domain are then derived. Solution of the integral equations yields the wavenumber domain solution for the pile rows. The space domain solution can then be retrieved by inversion of the Floquet transform. Numerical results show that the proposed model with the Floquet transform method is in a good agreement with those of the conventional direct superposition method. On the basis of the new model, influences of the spacing between neighboring piles, the Young's modulus of the piles, and the pile length on the vibration isolation effect of the pile rows are investigated. Numerical simulations conducted in this study show that compared with the direct superposition method, the efficiency of the proposed model for simulation of the vibration isolation via pile rows is very high. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
80.