Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase encoding gene inGracilaria/Gracilariopsis lemaneiformis

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.     

Timing analysis of the black hole candidate EXO 1846-031 with insight-HXMT monitoring

We present the observational results from a detailed timing analysis of the black hole candidate EXO 1846-031 during its outburst in 2019 with the observations ...     

Detection of a high-confidence quasi-periodic oscillation in radio light curve of the high redshift FSRQ PKS J0805–0111

In this work,we have searched for quasi-periodic oscillations(QPOs)in the 15 GHz light curve of the FSRQ PKS J0805-0111 monitored by the Owens Valley Radio Observatory(OVRO)40 m telescope during the period from 2008 January 9 to 2019 May 9,using the weighted wavelet Z-transform(WWZ)and the Lomb-Scargle Periodogram(LSP)techniques.This is the first time to search for a periodic radio signal in the FSRQ PKS J0805-0111 by these two methods.Both methods consistently reveal a repeating signal with a periodicity of 3.38±0.8 yr(>99.7%confidence level).In order to determine the significance of the periods,the false alarm probability method was applied,and a large number of Monte Carlo simulations were performed.As possible explanations,we discuss a number of scenarios including the thermal instability of thin disks scenario,the spiral jet scenario and the binary supermassive black hole scenario.We expect that the binary black hole scenario,where the QPO is caused by the precession of binary black holes,is the most likely explanation.FSRQ PKS J0805-0111 thus could be a good binary black hole candidate.In the binary black hole scenario,the distance between the primary black hole and the secondary black hole is about 1.71×10¹⁶ cm.     

基于新生代活动构造背景的地震重点危险区域综合研究——以安徽为例

基于地震的发生与断裂的活动性密切相关,为了科学合理并尽可能准确地判定未来地震重点危险区域,以安徽为例,利用由深浅部对比、宏微观构造解析所给出的新生代构造活动性最新研究成果,结合地震活动性资料研究结果,对一些重点部位的地震危险性进行了综合研究。结果表明,根据长时间尺度的地质构造最新研究揭示的活动性较强区域,与根据近代地震活动性资料研究反映的危险性较大区域,呈现较好的一致性特征,从而为未来地震重点危险区域的判定提供了新的较为可靠的依据和方法。     

山东省地震台网中心网站建设

主要介绍了山东省地震中心网站的页面栏目设计和页面具体内容,介绍了网站的具体功能,以及网站制作的技术与方法,通过访问该网站可以了解到山东省地震台网中心的各种信息,并且能够查询到山东省2002年以来大于2.0级的地震.     

Building damage in Dujiangyan during Wenchuan Earthquake

A field damage survey of 1,005 buildings damaged by the Wenchuan Earthquake in Dujiangyan City was carried out and the resulting data was analyzed using the statistical method. It is shown that buildings that were seismically designed achieved the desired seismic fortification target; they sustained less damage than the non-seismically designed buildings. Among the seismically designed buildings investigated, RC frame buildings performed the best in terms of seismic resistance. Masonry buildings with a ground story of RC frame structure were the second best, and masonry buildings performed the worst. Considering building height, multistory buildings sustained more severe damage than high-rise buildings and 2- and 3-story buildings. Compared to residential buildings, public buildings, such as schools and hospitals, suffered more severe damage.     

海洋动物体石油烃污染评价标准参考值的探讨

目前,国内外尚无颁布任何海洋生物石油污染评价标准,为了满足海洋生物石油污染评价的需要,本文采用综合分析与毒理学分析相结合的方法,探讨和提出了海洋动物石油污染评价标准参考值,海洋鱼类、贝类和甲壳类的石油污染评价标准参考值（干重）分别为１５、７０ 和２５ ｍ ｇ／ｋｇ。     

Attribution of trends in meteorological drought during 1960–2016 over the Loess Plateau,China

This study uses two forms of the Palmer Drought Severity Index(PDSI), namely the PDSI_TH(potential evapotranspiration estimated-by the Thornthwaite equation) and the PDSI_PM(potential evapotranspiration estimated by the FAO Penman-Monteith equation), to characterize the meteorological drought trends during 1960–2016 in the Loess Plateau(LP) and its four subregions. By designing a series of numerical experiments, we mainly investigated various climatic factors' contributions to the drought trends at annual, summer, and autumn time scales. Overall, the drying trend in the PDSI_TH is much larger than that in the PDSI_PM. The former is more sensitive to air temperature than precipitation, while the latter is the most sensitive to precipitation among all meteorological factors. Increasing temperature results in a decreasing trend(drying) in the PDSI_TH, which is further aggravated by decreasing precipitation, jointly leading to a relatively severe drying trend. For the PDSI_PM that considers more comprehensive climatic factors, the drying trend is partly counteracted by the declining wind speed and solar radiation. Therefore, the PDSI_PM ultimately shows a much smaller drying trend in the past decades.     

宁阳县蒋集镇水库土坝非稳定性探析

宁阳县蒋集镇地处汶河南岸,黄淮分水岭北侧,最大海拔高程361m.全镇区域面积12．7万km^2,其中山区、丘陵占2／3,岩石类型以花岗片麻岩为主。20世纪50-60年代兴建了117座水库塘坝,其中小（一）型水库2座,小（二）型水库15座,塘坝100座,总库容1700余万m^2,坝体以土坝为主。由于当时施工机械落后,技术有限,经费不足,造成工程质量较低,因而不少土坝产生渗流、流土、管涌、滑坡等现象。     

茯苓酸调节AMPK/GSK-3β/Nrf2信号通路对子宫内膜异位症大鼠内膜基质细胞铁死亡的影响

目的：探索茯苓酸调节腺苷酸活化蛋白激酶（AMPK）/糖原合成酶激酶3β（GSK-3β）/核因子E2相关因子2（Nrf2）信号通路对子宫内膜异位症（EM）大鼠基质细胞铁死亡的影响。方法：将51只SD大鼠于腹腔内异位移植子宫内膜组织构建EM大鼠模型,并将其随机分为模型组、茯苓酸低剂量（3.5 mg/kg）组、茯苓酸高剂量（7.0 mg/kg）组、茯苓酸高剂量（7.0 mg/kg）+盐酸多索啡（0.2 mg/kg）组,每组各12只,另取12只SD大鼠只开腹不移植,设为假手术组,药物分组处理后测定各组大鼠异位子宫内膜体积、异位子宫内膜组织铁死亡指标［谷胱甘肽（GSH）、丙二醛（MDA）水平、铁含量］、血清及异位子宫内膜组织炎症因子［肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）］水平、异位子宫内膜组织铁死亡相关蛋白［长链脂酰辅酶A合成酶4（ACSL4）、环加氧酶2（PTGS2）］与AMPK/GSK-3β/Nrf2通路相关蛋白表达。原代培养EM大鼠子宫内膜基质细胞,并将其随机分为对照组、茯苓酸低浓度（40 mg/L）组、茯苓酸高浓度（80 mg/L）组、茯苓酸高浓度（80 mg/L）+盐酸多索啡（10 μmol/L）组,以药物分组处理后,以CCK-8法、流式细胞术分别测定各组细胞增殖及凋亡;以试剂盒检测各组细胞铁死亡指标GSH及MDA水平、铁含量;以免疫印迹实验检测各组细胞铁死亡相关蛋白及AMPK/GSK-3β/Nrf2通路相关蛋白表达。结果：与假手术组相比,模型组大鼠异位子宫内膜体积、MDA水平及铁含量、血清及异位子宫内膜组织TNF-α及IL-6水平、ACSL4及PTGS2蛋白表达升高（P<0.05）,GSH水平、p-AMPK/AMPK、p-GSK-3β/GSK-3β及Nrf2蛋白表达降低（P<0.05）;与模型组相比,茯苓酸低、高剂量组大鼠异位子宫内膜体积、MDA水平及铁含量、血清及异位子宫内膜组织TNF-α及IL-6水平、ACSL4及PTGS2蛋白表达降低（P<0.05）,GSH水平、p-AMPK/AMPK、p-GSK-3β/GSK-3β及Nrf2蛋白表达升高（P<0.05）,且高剂量茯苓酸作用更强;盐酸多索啡可减弱高剂量茯苓酸对EM模型大鼠的作用。与对照组相比,茯苓酸低、高浓度组大鼠子宫内膜基质细胞活力、MDA水平及铁含量、ACSL4及PTGS2蛋白表达降低（P<0.05）,凋亡率、GSH水平、p-AMPK/AMPK、p-GSK-3β/GSK-3β及Nrf2蛋白表达升高（P<0.05）,且高浓度茯苓酸作用更强;盐酸多索啡可减弱高剂量茯苓酸对EM大鼠内膜基质细胞的作用。结论：茯苓酸可减轻EM大鼠炎症及铁死亡,抑制其子宫内膜基质细胞增殖及异位内膜生长,其作用机制可能是通过激活AMPK/GSK-3β/Nrf2信号通路来实现的。