产新琼四糖的海洋微泡菌属AG1热稳定性β-琼胶酶的过量表达与鉴定

从微泡菌属AG1（Microbulbifer sp. AG1）克隆得到1302 bp大小的琼胶酶基因,该基因编码产物为一个成熟蛋白（413个氨基酸残基）外加一个信号肽（20个残基）。将不含信号肽片段的琼胶酶在E. coli BL21 （DE3）中进行了异源表达和纯化。使用琼脂糖作为底物,该重组琼胶酶的最适反应温度和pH分别为60℃和7.5。该重组酶表现出优良的热稳定性,在50℃和60℃下处理1 h,重组琼胶酶仍能分别保持67%和19%的残余酶活力。除了SDS,重组琼胶酶对于其他测试的抑制剂、去垢剂和尿素变性剂有着较好的抗性。利用薄层色谱和以对硝基苯-α/β-D-吡喃半乳糖苷为底物的酶活力分析结果表明,该重组琼胶酶为β型琼胶酶,它水解琼脂糖的主要终产物为新琼四糖,而且不同聚合度的酶解产物具有抗氧化活性。     

Effect of Shale Reservoir Characteristics on Shale Oil Movability in the Lower Third Member of the Shahejie Formation,Zhanhua Sag

To reveal the effect of shale reservoir characteristics on the movability of shale oil and its action mechanism in the lower third member of the Shahejie Formation(Es3l), samples with different features were selected and analyzed using N2 adsorption, high-pressure mercury injection capillary pressure(MICP), nuclear magnetic resonance(NMR), high-speed centrifugation, and displacement image techniques. The results show that shale pore structure characteristics control shale oil movability directly. Movable oil saturation has a positive relationship with pore volume for radius > 2 μm, as larger pores often have higher movable oil saturation, indicating that movable oil is present in relatively larger pores. The main reasons for this are as follows. The relatively smaller pores often have oil-wetting properties because of organic matter, which has an unfavorable effect on the flow of oil, while the relatively larger pores are often wetted by water, which is helpful to shale oil movability. The rich surface provided by the relatively smaller pores is beneficial to the adsorption of immovable oil. Meanwhile, the relatively larger pores create significant pore volume for movable oil. Moreover, the larger pores often have good pore connectivity. Pores and fractures are interconnected to form a complex fracture network, which provides a good permeability channel for shale oil flow. The smaller pores are mostly distributed separately;thus, they are not conducive to the flow of shale oil. The mineral composition and fabric macroscopically affect the movability of shale oil. Calcite plays an active role in shale oil movability by increasing the brittleness of shale and is more likely to form micro-cracks under the same stress background. Clay does not utilize shale oil flow because of its large specific surface area and its block effect. The bedding structure increases the large-scale storage space and improves the connectivity of pores at different scales, which is conducive to the movability of shale oil.     

安徽巢湖地区中二叠统栖霞组的遗迹化石特征及其沉积环境

在安徽巢湖177高地、无为白牡山等地中二叠统栖霞组顶部灰岩段和臭灰岩段发现大量遗迹化石Zoophycos cf.caudagalli。这些遗迹化石以系统觅食迹为主,指示水动力及波浪作用较弱、贫氧的深水沉积环境。在栖霞组的砾状灰岩中,发现部分Zoophycos穿插于灰岩砾石和基质之中,这揭示栖霞组石灰砾岩中的砾石并非成岩作用成因,而是碎屑流沉积形成,属于异地成因碳酸盐岩。综合巢湖地区栖霞组的地层和岩石地球化学特征以及Zoophycos的生活习性和分布特征,发现表明巢湖地区中二叠统栖霞组臭灰岩段和顶部灰岩段属于贫氧的碳酸盐台缘斜坡沉积。     

岷县漳县6.6级地震前区域地壳运动变形背景与断层形变异常特征

利用甘肃岷县漳县6.6级地震区所在的青藏块体东北缘地区的区域水准、GPS、流动重力和跨断层短测线等地形变监测资料,结合地质构造、动力环境和已往的研究结果,分析了不同类型资料反映的震前区域性地壳变形背景、断层形变异常特征和可能的机理.结果认为：（1）岷县漳县6.6级地震前西秦岭、六盘山等构造区不同程度地存在着GPS水平挤压闭锁高应变积累、垂直隆升异常高梯度带和重力升、降差异剧烈变化等中长期背景;（2）震前到震时发震断裂附近及其外围相关构造区域断层形变异常在空间和时间上的起伏波动变化显著,尤其是汶川地震以来的波动变化在一定程度上反映了与本区构造比邻的龙门山断裂带剧烈右旋错动对本区的影响,与本次岷县6.6级地震过程有关;（3）本区有地形变监测资料积累以来缺乏6级以上震例,虽然存在不同程度的中长期形变背景异常,但何时进入短期-短临阶段确实很难把握,需要不断积累总结和探索提高.     

Wide area real time kinematic decimetre positioning with multiple carrier GNSS signals

This paper presents the technical basis for wide area real time decimetre positioning services using multiple carrier signals transmitted by future GNSS such as modernized GPS and Compass systems. The first step is to form two ionosphere-reduced extra-widelanes (EWL) that have the minimal total noise levels in cycles, considering the effects of the ionospheric and tropospheric delays, orbital error, and phase noise terms in various observational environments. The proposed three carrier ambiguity resolution ...     

The Linkage between Midwinter Suppression of the North Pacific Storm Track and Atmospheric Circulation Features in the Northern Hemisphere

The midwinter suppression(MWS) of the North Pacific storm track(NPST) has been an active research topic for decades. Based on the daily-mean NCEP/NCAR reanalysis from 1948 to 2018, this study investigates the MWS-related atmospheric circulation characteristics in the Northern Hemisphere by regression analysis with respect to a new MWS index, which may shed more light on this difficult issue. The occurrence frequency of the MWS of the upper-tropospheric NPST is more than 0.8 after the mid-1980 s. The MWS is accompanied by significantly positive sea-level pressure anomalies in Eurasia and negative anomalies over the North Pacific, which correspond to a strengthened East Asian winter monsoon. The intensified East Asian trough and atmospheric blocking in the North Pacific as well as the significantly negative low-level air temperature anomalies, lying upstream of the MNPST, are expected to be distinctly associated with the MWS. However, the relationship between the MWS and low-level atmospheric baroclinicity is somewhat puzzling.From the diagnostics of the eddy energy budget, it is identified that the inefficiency of the barotropic energy conversion related to the barotropic governor mechanism does not favor the occurrence of the MWS. In contrast, weakened baroclinic energy conversion, buoyancy conversion, and generation of eddy available potential energy by diabatic heating are conducive to the occurrence of the MWS. In addition, Ural blocking in the upstream region of the MNPST may be another candidate mechanism associated with the MWS.     

CX盆地露头岩样电阻率测试与分析

本次实验的主要目的是对ＣＸ地区露头完好的岩样进行电阻率测试,提出了一种利用露头岩样电阻率绘制模拟地层条件下仿真电阻率测井曲线的新方法。该方法的主要思路是：（１）在ＣＸ地区选取一条露头出露较完好的地质剖面,在野外进行详细的岩性、地层产状和厚度描述,并采集露头岩石标本。（２）对所取岩样进行常温常压下的密度、孔隙度和电阻率测试,并选部分代表一定岩性的岩样进行模拟地层环境（压力、饱和度、孔隙度）下的电阻率     

由空间直角坐标计算大地坐标的简便公式

对大地测量教科书中给出的空间三维直角坐标与大地坐标的转换公式进行了大量的计算与分析，总结得出一组由空间直角坐标计算大地坐标的简便公式。     

锦屏电站坝厂区卸荷岩体力学参数研究

选择具有代表性的区块,根据结构面的性状和规模拟定模型层次和尺寸,建立岩体数值仿真模型,通过计算机数值仿真,采用卸荷非线性力学理论对锦屏电站坝厂区岩体进行卸荷岩体宏观力学特性及参数的数值分析;同时,用RMR法和BP神经网络法对岩体宏观力学特性及参数进行分析,综合确定岩体力学参数,为设计部门提供设计参考依据.     

三维地震数据离散光滑插值的共轭梯度法

针对三维地震数据插值,提出采用Laplacian算子进行光滑约束的插值方法,并借鉴Mallet研究的离散光滑插值思路,采用预条件共轭梯度法,直接生成网格节点上的值,从而回避寻求满足插值方程的函数. 为了实现其中Laplacian算子的快速求逆,文中引入Claerbout螺旋坐标系谱因式分解理论. 在螺旋坐标系下,Laplacian算子的表示矩阵具有Toeplitz结构,其快速求逆可由谱法LU分解实现. 基于二维离散光滑插值,文中还给出共轭梯度法与NMO相结合的沿时间切片逐层处理的离散光滑插值流程. 最后,应用该方法对模型数据和实际三维地震数据进行了处理.