人工饲料饲养的对虾肠道菌群和水体细菌区系的研究

对人工饲料饲养的凡纳滨对虾Litopenaeus vannamei成虾肠道菌群组成及其水体细菌区系组成进行了研究.分离纯化后的革兰氏阴性菌鉴定到种的水平.对虾肠道菌群主要由弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、嗜氢菌属Hydrogenophaga spp.、伯克霍尔德氏菌Burkholderia spp.、气单胞菌Aeromonas spp.、食酸菌Acidovorax spp.和芽孢杆菌Bacillus spp.组成.水体细菌区系主要包括弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、艾肯菌Empedobacter spp.、无色杆菌Achromobacter spp.和芽孢杆菌Bacillus spp..肠道菌群和水体细菌区系的优势菌是副溶血弧菌Vibrio parahaemolyticus和芽孢杆菌Bacillus spp..利用Shannon-Wiener指数进行多样性分析,发现虾肠道多样性指数（H）和均匀度（J）高于水体细菌区系,而丰度（D）较低.     

罗非鱼、南美白对虾对重金属富集的研究

对广东省养殖的罗非鱼Oreochromis niloticus及南美白对虾Litopeneaus vannamei体内Pb、Cd、Hg、As 4种重金属富集及分布状况进行研究。比较了不同规格罗非鱼及南美白对虾体内重金属差异。分析重金属在罗非鱼及南美白对虾不同部位的分布状况。结果表明,罗非鱼及南美白对虾体内Pb、Cd、Hg、As均有不同程度的检出,但含量均低于国家限定标准(NY5125-2002);其中Pb的含量最高,罗非鱼和南美白对虾体内Pb含量最高值分别为限量的39.32%和42.18%;重金属含量因其规格大小及部位而异,趋势为大规格样品重金属含量相对较高,重金属含量在罗非鱼及南美白对虾体内的增长情况与其体重具有正相关性;罗非鱼内脏重金属含量明显高于肌肉组织,虾头为虾重金属富集的主要部位。     

近岸溢油漂移扩散预测方法研究——以胶州湾溢油事件为例

近些年,由于中国经济的高速发展,原油需求逐年增长,同时海上溢油事故发生的风险也在加剧。海上溢油会严重破坏海洋环境,危害我国经济发展,加强溢油漂移扩散预报研究能够为海上溢油应急响应提供技术支撑。因此,国家海洋信息中心研发了海上突发事件应急一体化预测预警系统。该系统基于GIS平台研发,能够在二维电子海图基础上叠加相关的海洋环境动力要素信息,模拟溢油扩散和漂移态势,同时计算剩余油量,估算溢油面积以及岸线吸附程度等。2013年11月22日,山东黄岛发生输油管道爆炸事故,造成大量原油溢入胶州湾。本文利用该系统结合高分辨率大气模型WRF的三重嵌套技术和海流模型SELFE的非结构化网格加密技术,对事故溢油漂移扩散开展了预测工作。预测结果显示,油污受胶州湾内往复流的影响极大,72 h后,胶州湾内外大部分海域都将存在油污,主要集中在黄岛沿岸海域、团岛及浮山湾附近,预测扫海面积为70.32 km2。经海事部门高分辨率卫星观测结果印证,系统模拟的溢油分布较为合理。该系统的业务化应用为相关海上溢油污染应急工作提供了一定理论依据和参考。     

Effect of free surface and strut on fins attached to a strut

An extensive experimental and computational investigation of the combined and separate effects of free surface and body on the lift characteristics of a pair of fins attached to a strut and fin alone is conducted. The results reveal that the free-surface effect becomes significant when the depth of submergence to chord ratio (H/c) is less than three. The effect of the strut is also realized for shallower depth of submergence of the fins through free-surface deformation leading to a significant change in the incidence angle of the flow to the fins. The numerical results based on the Higher Order Boundary Element Method with the linearized free-surface condition show good agreement with the experimental results for fin (foil) alone even at shallow submergence, but some discrepancies appear for the fin attached to the strut at higher speeds mostly due to the neglect of the nonlinear free-surface effect.     

ELASTIC CLASSIFICATION OF MODIFIED WATER MASS IN SHALLOW SEA

In this paper, the principle and steps for differentiating water masses by fuzzy cluster method are introduced, and a scalar formula based on Euclidean distance and a method for determining objectively the number of water masses by F-test are proposed. Consequently, a method and specific steps for differentiating modified water masses in shallow sea according to fuzzy elastic classification are given. Computation of the membership degree in which each sample belongs to every water mass determines conveniently and quantitatively the cores, boundaries of water masses and mixed zones. An example for the Huanghai Sea and East China Sea is shown and compared with previous results.     

A Linear Programming Model for Seabed Oil-Gas Pipe Network

This paper analyzes the pipe network system of oil-gas collection and transportation foroffshore oilfield development.A"0-1"integer linear programming model is constructed to optimize theinvestment of seabed pipe network.The mathematical model is solved by the spanning tree method ofgraph theory and network analysis.All spanning trees of a network graph compose all the feasible solu-tions of the mathematical model.The optimal solution of the model is the spanning tree with the minimumcost among all spanning trees.This method can be used to optimize the seabed pipe network system andgive a minimum cost plan for the development of offshore marginal oilfield groups.     

Embryonic and larval development of Babylonia formosae habei (Altena and Gittenberger, 1981) (Gastropoda:Buccinidae) on China''''s coast

The development of embryos and larvae of Babylonia formosae habei living along the southeast coast of China is observed under laboratory conditions. The egg masses are laid by females on hard substrate at night and each capsule contains 100-500 eggs. Each egg is 250-280 mm in diameter. The first two cleavages of the embryo are meridional and equal, and a polar lobe is produced. Larval kidney, which only consists of a single cell, appears during the gastrula stage on each side of the embryo. The right tentacle develops prior to the left one. At 25-27℃, an intracapsulate veliger stage is reached about 4.5 d after deposition. The larvae hatch on the fifth day as swimming veligers with a shell length of 360 -500 mm. The newly hatched larva can ingest suspended algal cells from the water column and remains in the pelagic stage for 8-10 d. The newly settled juveniles are 900-1 200 mm in shell length.     

混凝土的渗透性与耐久性

渗透性是混凝土耐久性中最重要的内容。论述了混凝土耐久性的影响因素、研究方法 ,以及混凝土渗透机理和渗透性与耐久性的关系。     

半潜式超大浮体连接器动力特性的一种时间序列分析方法

根据刚性模块弹性连接器 (RMFC)假设研究由 3个模块连接而成的半潜式超大浮体MobileOffshoreBase(MOB)的波浪载荷动力响应。模块和连接器动力响应根据时间序列法计算 ,这种方法包括海况模拟 ,波浪载荷计算和结构响应计算三个主要部分。通过对结构的适当简化 ,根据Airy波理论 ,Morrison公式以及流体中的结构运动方程、弹性连接器变形方程 ,计算求得MOB在不规则海况下的动力响应 ,并将其与相关文献中的结果进行比较 ,表明该方法有良好的精确性     

基于模拟退火算法的饲料配方设计

本文在饲料配方设计中首次引入新颖、高效的组合优化算法--模拟退火算法，并获得理想的数值结果，说明了在传统的饲料配方设计中高技术的应用可以获得更高的效益.