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81.
人工诱导雌核发育是水产动物性别控制的重要技术手段之一,同时也是快速建立近交系或纯系、建立遗传作图群体的方法之一。采用静水压力抑制受精卵第一次卵裂的方法诱导了牙鲆同质雌核发育二倍体。不同的处理起始时刻、不同压力及压力持续的时间对受精卵的发育及染色体加倍率有较大影响。处理组受精卵发育相对缓慢,畸形率较高,正常仔鱼孵化率较低。如果处理前受精卵保持在(15.5±0.5)℃,压力处理受精卵的最佳条件是:从受精后75 min开始、用55 MPa的压力处理6 min,该条件下处理组正常仔鱼孵化率较高,为13.7%,染色体观察和最大核仁数计数结果表明,此时的二倍体诱导率也最高。染色体观察结果还显示在不同处理组中存在不同比例的非整倍体。利用获得的最佳诱导条件处理获得了数千尾雌核发育幼鱼,观察表明,雌核发二倍体鱼的生长速度明显低于普通二倍体,且个体间差异较大。该研究为进一步开展牙鲆全雌育种和连锁图谱的构建提供技术支撑。 相似文献
82.
采用高分辨折射和宽角反射/折射综合地震探测方法,在浙江省中部沿黄岩、临海、东阳、桐庐、昌化进行深部构造探测,获得一条长约350km的速度结构剖面。探测结果表明,地壳结构具有明显的多层性,存在3个速度间断面;基底速度结构图像揭示了速度结构的横向非均匀性,这种不均匀性既对应于地质构造单元的分界线或断裂构造带,也反映出东西部构造特征的差异性。 相似文献
83.
研究并实现中国区域北斗广域差分实时电离层延迟格网改正算法,针对北斗单系统实时建立电离层延迟格网的不足,提出联合BDS/GPS的北斗广域差分实时电离层延迟改正方法。比较BDS、GPS以及BDS/GPS观测的实时电离层延迟格网,并利用北斗单频单点伪距定位进行精度验证。结果表明,在有效格网点覆盖充足的地区,BDS与GPS实时电离层延迟格网定位效果相当;而联合BDS/GPS观测的实时电离层延迟格网极大提高了偏远地区的单频定位精度与可定位历元数,也使原本单系统格网覆盖充足的地区定位效果得到进一步增强和稳定。 相似文献
84.
Drilling fluid is a common flushingmedium used in pile foundation, geological drilling and petroleum drilling. Study on ultrasonic propagation properties in drilling fluid is of vital importance, not only for developing equipments to non-contact measuring concrete casting level for bored pile, but also for developing equipments considering drilling fluid as signal channel. The existence of clay particlesmakes the ultrasonic propagation and attenuation in drilling fluid much different from pure water. In order to know the relation among ultrasound frequency, slurry density and depth, a series of laboratory experiments about ultrasound propagation in water-based bentonite slurry were finished. Wavelet method was adopted to process the gained original waves of ultrasonic propagation in slurry, so we knew the velocity and attenuation coefficient of ultrasound propagated in different drilling fluids with different density. The first group experiments shows that with density of drilling fluid increase, ultrasonic velocity will decrease but attenuation coefficient will increase if ultrasonic frequency keep constant. The second group experiments shows that the power of ultrasound will intensify in small bore hole, the attenuation coefficient ismuch smaller than theoretical value. 相似文献
85.
Accumulation of petroleum hydrocarbons and heavy metals in clams (Ruditapes philippinarum) in Jiaozhou Bay, China 总被引:2,自引:0,他引:2
Accumulation and distributions of aliphatic and polyaromatic hydrocarbons (PAHs) and heavy metals were measured in tissues
of the clam Ruditapes philippinarum collected from 5 sites in Jiaozhou Bay, Qingdao, China. The concentrations of total aliphatic hydrocarbon and PAHs ranged
from 570 to 2 574 ng/gdw (gram dry weight) and from 276 to 939 ng/gdw, in the most and least polluted sites, respectively.
The bio-accumulation of hydrocarbons and PAHs in the clams appeared to be selective. Aliphatic hydrocarbons were predominantly
represented by short chain (<nC23) n-alkanes, suggesting that petroleum hydrocarbons were likely the major contamination source. The selective uptake of 3 and
4 ring PAHs, such as naphthalene, fluorene, phenanthrene, fluoranthene and pyrene, by the clams was probably related to the
physiological and bio-kinetic processes that were energetically favorable for uptake of compounds with fewer rings. Accumulation
of the metals Cd, Cu, Zn, Pb, Cr, Hg, and As in the clam tissues also showed high variability, ranging from 0.043 to 87 μg/gdw.
Among the 7 detected metals, Zn, Cd, Cu, and As had a particularly high potential of accumulation in R. philippinarum. In general, a positive correlation was found between the tissue concentrations and sediment concentrations of hydrocarbons
and of some metals. Our study suggests that moderate contamination with polyaromatic hydrocarbons, and low to moderate contamination
with metals, currently exists for clam R. philippinarum in Jiaozhou Bay, in comparison with other regional studies. A long-term monitoring program is certainly needed for assessment
of the potential ecological influence and toxicity of these contaminants of R. philippinarum in Jiaozhou Bay. 相似文献
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89.
为探究不同测量方法获取浅沟数据的精度,本文以元谋干热河谷区浅沟为例,采用RTK、三维激光扫描、近景摄影测量等多种测量方法获取浅沟数据,建立DEM并提取浅沟形态参数,对比近景摄影测量以不同方式获取标记坐标后建立DEM的差异,同时以测针板实测浅沟横剖面为参照,对比分析不同测量方法获取浅沟横剖面的精度。结果表明:RTK获取密度较低的点数据,建立的DEM较粗糙,仅体现沟缘大致走向;近景摄影测量+RTK方法测得的DEM高程整体低于近景摄影测量+全站仪所得DEM高程;利用三维激光扫描仪和近景摄影测量+全站仪两种方法获取数据所提取的浅沟形态参数精度较高;对比浅沟横剖面,横剖面近景摄影测量+全站仪测量所得与实测横剖面重合度较高。因此,利用近景摄影测量+全站仪进行浅沟测量,在精度、效率等方面优势较大。 相似文献
90.
This study reveals that the interannual variability of the western edge of the western North Pacific(WNP)subtropical high(WNPSH) in early summer experienced an interdecadal decrease around 1990. Correspondingly, the zonal movement of the WNPSH and the zonal extension of the high-pressure anomaly over the WNP(WNPHA) in abnormal years possess smaller ranges after 1990. The different influences of the tropical SSTAs are important for this interdecadal change, which exhibit slow El Nino decaying pat... 相似文献