绿鳍马面鲀(Navodon septentrionalis)CYP19a基因克隆与表达特征分析

利用简并引物扩增及RACE全长克隆技术, 克隆得到绿鳍马面鲀(Navodon septentrionalis)CYP19a基因cDNA全长序列。通过多序列比对, 发现具有芳香化酶特定保守序列, 包括一个I-螺旋区, 一个Ozol肽区, 一个亚铁血红素结合区域以及一个芳香化酶特异性结合区域。通过RT-PCR技术检测了其在绿鳍马面鲀成鱼各组织表达的情况, 发现其CYP19a基因只在卵巢中有表达; 同时也分析了其在不同卵巢发育期的表达情况, 发现CYP19a在卵黄发生后期表达量达到最高值, 卵巢退化吸收期表达量达到最低值。     

Turning depths of internal tides in the South China Sea inferred from profile data

Theoretically, propagating internal tides in the ocean may reflect at turning depths, where buoyancy frequencies equal tidal frequencies, before colliding with the air-sea interface or rugged bottom topography. Globally, the internal tide lower turning depths（ITLTDs） in the open ocean have been mapped; however, knowledge of the presence of ITLTDs in the South China Sea（SCS） is lacking. In this study, 2 125 high-quality temperature-salinity profiles（including 58 deep-sea hydrographic measurements...     

基于多尺度有限元的海上升压站脚靴式桩套筒连接结构分析研究

脚靴式海上升压站导管架的桩套筒与主导管的连接结构是关键荷载传递部位。整体梁系有限元模型无法反应局部板型结构的失效状态;截断板系有限元模型则受制于边界条件的合理性,结果不够精确。文中基于某工程海上升压站,建立不同精细化范围的多尺度有限元模型及不同边界条件的截断模型,对结构屈服失效及屈曲失效复核的同时论证了局部网格对计算精细程度的影响及截断模型对边界条件的敏感性;此外,研究了轭板板厚及应力比对屈曲失效的敏感程度,为今后海上升压站脚靴结构不同阶段的设计、优化提供参考和借鉴。     

缺少已知控制点时的平面控制测量方法研究

在目前海洋测绘中,平面控制测量主要采用静态同步观测方式,通过联测不少于2个已知控制点实施.为探究少于3个已知控制点时能否有效开展平面控制测量,结合工程试验,采用RTX后处理(RTX-PP)技术获得控制点ITRF2014框架当前历元坐标,然后根据已知控制点数量情况,分别采用四参数平面坐标转换、坐标平移转换、框架及历元转换...     

用于海底目标识别与底质分类的多波束水体波形预处理

多波束水体数据是多波束系统获取的最原始数据,记录了波束从发射到接收整个过程全部的反向散射强度信息,可以为目标识别、水下栖息环境探测等提供重要的数据支撑.目前,针对多波束水体强度时间序列所表现的波形信息的处理及研究仍处于起步阶段,另外水体波形数据易受噪声影响,且存在明显的入射角效应问题,对此,本文提出了一种基于分区异构的...     

MIKE21模型在海洋工程研究中的应用

介绍了MIKE21数学模型的数学基础和计算方法,将MIKE21应用于山东省龙口湾人工岛群工程开发对海洋环境的影响研究中,并就其中潮流场的模拟过程做出详细说明.通过与研究区多个站位的实测结果对比显示,计算域潮流场预测结果较为合理,表明MIKE21软件对于此类问题有较高的实用价值.将水动力数值模拟全过程设计成软件包,可使其通用性大大加强,是未来水动力数值模拟发展的方向.     

东海陆架北部泥质区沉积动力过程的季节性变化

利用东海陆架北部泥质区（济州岛西南）及其周围不同季节５个航次的悬浮体资料和相应的底质粒度资料,对该泥质区沉积动力过程的季节性变化规律进行了分析。结果表明：东海陆架北部悬浮体的高含量区并不与该区的沉积中心（泥质区）相吻合,泥质区的形成需有物源的充沛供应和涡旋的动力效应相结合。泥质区沉积作用时空变化具有强烈的季节性。冬季泥质区悬浮体供应充足,涡旋中心向泥质区东南扩展,是本区接受悬浮体沉积的关键季节。夏、秋二季泥质区的大部分区域缺少悬浮体物源,涡旋中心向北退缩,致使泥质区沉积作用强度降低,并且主要影响泥质区的西北区域。春季泥质区沉积作用强度和影响范围大于夏、秋两季,但小于冬季。     

本刊加入万方数据(ChinaInfo)系统科技期刊群的声明

为了实现科技期刊编辑、出版发行工作的电子化,推进科技信息交流的网络化进程,我刊现已入网"万方数据(ChinaInfo)系统科技期刊群",所以,向本刊投稿并录用的稿件文章,将一律由编辑部统一纳入万方数据(ChinaInfo)系统,进入因特网提供信息服务.凡有不同意者,请另投它刊.本刊所付稿酬包含刊物内容上网服务报酬,不再另付.     

Phylogenetic diversity of planktonic bacteria in the Chukchi Borderland region in summer

Planktonic bacteria are abundant in the Chukchi Borderland region. However, little is known about their diversity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization (FISH) showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic (AAP) bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta and Gamma subclasses), Bacteroidetes, Actinobacteria and Firmicutes. No significant difference was found between the two clone libraries. SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammapro-teobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell into Gammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFrac data showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea.     

A Global Reliability Assessment Method on Aging Offshore Platforms with Corrosion and Cracks

Corrosion and fatigue cracks are major threats to the structural integrity of aging offshore platforms. For the rational estimation of the safety levels of aging platforms, a global reliability assessment approach for aging offshore platforms with corrosion and fatigue cracks is presented in this paper. The base shear capacity is taken as the global ultimate strength of the offshore platforms. It is modeled as a random process that decreases with time in the presence of corrosion and fatigue crack propagati...