Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

渤海生态动力过程的模型研究 Ⅱ.营养盐以及叶绿素a的季节变化

利用在本系列研究第一部分中所建立的耦合的生物物理模型,模拟了渤海浮游植物生物量和营养盐含量的年度循环特征.模拟结果显示:藻类的春季水华是由经过一冬积累在水体中的营养盐导致,而水华开始的时间在浅水区明显早于深水区,对此深水区水体层化结构的形成可能起着重要作用;另一方面,河载营养盐与悬起的沉积物所释放的营养盐是诱发夏季水华的共同原因.基于模型结果,我们还发现:渤海的浮游植物动力特性就整体而言依然受无机氮限制,但是在莱州湾,磷限制特性表现得非常明显,这主要是由于每年黄河都要携带大量的无机氮进入海水,从而导致莱州湾营养盐的氮磷比已远远超过16.     

3种蛏类线粒体16SrRNA和COI基因片段的序列比较及其系统学初步研究

对3种蛏类大竹蛏（Solen grandis），长竹蛏（Solen strictus）和小刀蛏（Cultellus attenuatus）的线粒体16SrRNA和COI基因片段序列进行了比较并对其系统学进行了初步研究。得到的序列总长度分别为472-481bp（16S）和658bp（COI）。3种蛏序列的碱基组成均显示出较高的A＋T比例（16SrRNA基因62．1％；COI基因62．8％）。对位排序比较表明，16SrRNA片段种内个体间变异较小，3种类间存在128个碱基变异位点（其中包括127个简约信息位点）和5个插入／缺失位点，总共12个碱基长；COI片段有200个碱基存在变异，其中包括191个简约信息位点，不存在任何插入／缺失位点。数据分析结果表明16SrRNA和COI基因片段大竹蛏与长竹蛏两片段的遗传距离分别为0．0856和0．1712，两竹蛏类与小刀蛏的遗传距离分别为0．3054，0．2798和0．2662，0．2933。作者认为小刀蛏与竹蛏之间的遗传距离已达到科之间的水平，结果支持将其提升为刀蛏科的分类观点。3种蛏类线粒体16SrRNA和COI基因在种间明显的多态性，证实了16SrRNA和COI基因序列均普遍适用于蛏类种及以上阶元的系统学分析。     

黄河尾闾河道1996年改道的意义及黄河三角洲演化趋势

现代黄河三角洲由几个亚三角洲组成。 1 85 5 -1 93 4年 ,形成了以宁海为顶点的第一亚三角洲 ;1 93 4-1 996年 ,三角洲冲积扇顶点进一步下移 ,形成了以渔洼为顶点的第二亚三角洲。最近的一次改道是在 1 996年 6月 ,改道后的河流沿清水沟流路的清 8剖面附近东流入海。这次改道可能是第三亚三角洲形成的开始。以后数十年内的黄河尾闾摆动将在清 8剖面附近进行。虽然人类活动将对三角洲的发展演化产生越来越明显的影响 ,但这些亚三角洲的发育演化仍有明显的规律性     

海浪波群的谱特征分析

海浪的波谱特征分析对认识海浪有重要的意义，因为海浪谱反映了海浪的结构成分。这对海工建筑、海洋运输、船舶制造、海浪预报和海浪模拟等均有重要的实用意义。 波群是伴随海浪经常出现的一种自然现象，且经常伴随大浪的出现而出现。实践证明，波群具有极大的破坏力，其破坏性远比单个波大得多，它是海浪破坏海工建筑物的重要因子，许多防波堤的破坏并非是由单个波的作用，而是由相继出现的几个大波(波群)作用的结果。早先，人们对波群这种习见的现象研究甚少。自1974年葡萄牙锡尼斯港深水防波堤的破坏之后，对波群的研究才日益受到重视。 对波群的研究目前多采用包络方法、马尔科夫链方法和波能过程线法。波群研究的主要内容是波群的统计特征(如波群链长统计)；另外，波群的模拟也是重要的研究内容之一。目前用包络方法研究波群比较普遍，且有不少研究成果。徐德伦等(2001)综述了这方面的研究进展及结果，文圣常等(1984)也进行了详尽的讨论。 以往的研究大都是研究波群的统计特征，如合田良实(1976)、 Ewing(1973)、Rye(1974)。而针对地域性的波浪，我国学者也只是以包络方法为基础讨论了不同地域海浪波群的统计特征(常德馥，1987；葛明达，1986；范顺庭，1998)。就波群本身的结构特征而言，即波群的波谱分析特征以往的研究涉及尚少。作者拟借助于谱分析方法，对带有典型波群的实测的海浪记录来分析海浪波群的波谱结构，进而借此对海浪波群的结构特征进行深入探讨。     

栉孔扇贝(Chlamys farreri)自然群体遗传多样性的RAPD分析

利用RAPD技术对栉孔扇贝(Chlamysfarreri)的四个自然群体(大连、烟台、青岛、韩国)的遗传多样性进行了分析。12个随机引物在四个群体中共检测到123个位点,其中多态位点109个,多态位点比例为88.62%;四个群体中均未检测到各群体特有的扩增带。韩国、烟台、大连、青岛四个群体的遗传多样性指数(Shannon指数)分别为0.2557、0.2557、0.2344和0.2249,以韩国和烟台群体遗传多样性较高,其次为大连群体,青岛群体最低,但它们之间无明显差异。不同群体的遗传多样性指数的平均数Hpop为0.2427,整个栉孔扇贝自然群体的遗传多样性指数Hsp为0.2503。遗传多样性剖分显示,绝大多数(97%)的遗传多样性是由群体内不同个体间的差异造成的,只有很小部分(3%)的遗传多样性与群体间的遗传分化有关。而采用基于单倍型(等位基因)之间的进化距离的AMOVA分析得到的这两个数据则分别为99%和1%,Φ值为0.010,且P=0.05,表明群体间遗传分化明显程度位于临界值。     

Bachelor-modon type eddies and isolated eddies near the coast on anf-plane

The behavior of isolated meso-scale eddies near the coastal boundary is studied by numerical experiments based on the quasi-geostrophic equation in a basin on an f-plane. First, Bachelor-modon type eddies are investigated as an idealized model of isolated eddies close to the wall. The first-mode Bachelor-modon type eddy is found to be robust enough to recover its original form even after it turns a corner of the basin. In contrast, the second mode is unstable; it tends to move away from the wall and finally splits into two eddies proceeding in opposite directions along the wall. An initially Gaussian eddy a little distant from the boundary interacts with a Bachelor-modon type eddy translating along the boundary, sometimes resulting in vortex merging and pairing just as in the head-on collision of two modons on a beta-plane. It is found that an initially Gaussian eddy located moderately close to the coast rapidly settles down to a steadily translating eddy, which can be approximated remarkably well by a first-mode Bachelor-modon type eddy not only in appearance but also in translation velocity within an error of about 20%.     

A note on internal wavetrains and the associated undulation of the sea surface observed upstream of seamounts

An internal wavetrain, generated by a tidal current in superposition with the Tsushima Warm Current, has been observed by use of an acoustic echo-sounder upstream of the Shichiri-Ga-Sone Seamounts in the East Tsushima Strait of the Japan Sea. The sea surface above the internal wavetrain was simultaneously observed and was found to be undulated at the wavelength of the internal wave.     

东海沿岸海区垂直环流及其温盐结构动力过程研究Ⅱ．温盐结构

对于本研究采用的动力学模型及其垂直环流的模拟结果已在第Ⅰ部分论述。作者将对与垂直环流对应的温、盐结构进行分析。温度和盐度模拟结果表明：冬季东海沿岸海区的温、盐分布均为近岸低、外海高;近岸温、盐呈垂直均匀分布,在外海出现分层,其温度为表层高、底层低,而盐度却为表层和底层高,中层偏低,长江口以南的近表层以下形成自近岸伸向外海的弱低盐水舌;长江冲淡水区及长江口以北和其以南外海的近表层有温、盐跃层生成,深底层温、盐呈均匀分布,且保持低温高盐特征;随着海区自北往南纬度的降低,岸坡和水深的增大及沿岸下降流的增强,温度和盐度自近岸至外海的垂直均匀分布跨度逐渐变窄;外海近表层的温、盐跃层强度自北至长江口逐渐增强,而自长江口至南逐渐减弱,其位置自北往南逐渐上移;冬季沿岸下降流使长江冲淡水区的盐跃层变厚。夏季海区的温度分布为近岸和外海高,近岸稍远出现冷水涌升,垂向上呈现显著分层,盐度分布为近岸低、外海高;长江冲淡水区及杭州湾以南外海的次表层存在温、盐跃层,其跃层以上出现混合层,且保持高温低盐特征,跃层以下温、盐大致呈均匀分布,并保持低温高盐特征;随着海区自南往北纬度的增高、岸坡和水深的减小及沿岸上升流自南至长江口和自长江口至北的增强和继而减弱,长江冲淡水区的温、盐跃层强度自南至长江口逐渐增强,而自长江口至北逐渐减弱,外海次表层的温、盐跃层强度却自南至长江口逐渐减弱,自长江口至北又逐渐增强,其温、盐跃层的位置自南往北逐渐上移;夏季沿岸上升流使长江冲淡水区的盐跃层变薄。     

Difference characteristics of sea surface temperature observed by GLI and AMSR aboard ADEOS-II

This study compares infrared and microwave measurements of sea surface temperature (SST) obtained by a single satellite. The simultaneous observation from the Global Imager (GLI: infrared) and the Advanced Microwave Scanning Radiometer (AMSR: microwave) aboard the Advanced Earth Observing Satellite-II (ADEOS-II) provided an opportunity for the intercomparison. The GLI-and AMSR-derived SSTs from April to October 2003 are analyzed with other ancillary data including surface wind speed and water vapor retrieved by AMSR and SeaWinds on ADEOS-II. We found no measurable bias (defined as GLI minus AMSR), while the standard deviation of difference is less than 1°C. In low water vapor conditions, the GLI SST has a positive bias less than 0.2°C, and in high water vapor conditions, it has a negative (positive) bias during the daytime (nighttime). The low spatial resolution of AMSR is another factor underlying the geographical distribution of the differences. The cloud detection problem in the GLI algorithm also affects the difference. The large differences in high-latitude region during the nighttime might be due to the GLI cloud-detection algorithm. AMSR SST has a negative bias during the daytime with low wind speed (less than 7 ms⁻¹), which might be related to the correction for surface wind effects in the AMSR SST algorithm.