Study on biogenic amines in various dry salted fish consumed in China

This study was carried out to investigate the biogenic amines（BAs）, physicochemical property and microorganisms in dry salted fish, a traditional aquatic food consumed in China. Forty three samples of dry salted fish were gathered from retail and wholesale markets and manufacturers, which had been produced in various regions in China. Cadaverine（CAD） and putrescine（PUT） were quantitatively the most common biogenic amines. About 14% of the samples exceeded the histamine content standards established by the FDA and/or EU. The highest histamine content was found in Silver pomfret（Pampus argenteus）(347.79 mg kg^-1). Five of forty three samples exceeded the acceptable content of TYR(100 mg kg^-1), and 23.26% of dried-salted fish contained high contents of biogenic amines(above 600 mg kg^-1). In addition, species, regions, pickling processes and drying methods made the physicochemical property, microorganisms and biogenic amines in dry salted fish to be different to some extents. The total plate count（TPC） was much higher than that of total halophilic bacteria in all samples. The biogenic amines, physicochemical property and microbiological counts exhibited large variations among samples. Furthermore, no significant correlation between biogenic amines and physicochemical property and TPC was observed. This study indicated that dry salted fish may still present healthy risk for BAs, depending on the processing methods, storage conditions among others.     

Advanced glycation endproducts in 35 types of seafood products consumed in eastern China

Advanced glycation endproducts(AGEs) have been recognized as hazards in processed foods that can induce chronic diseases such as cardiovascular disease, diabetes, and diabetic nephropathy. In this study, we investigated the AGEs contents of 35 types of industrial seafood products that are consumed frequently in eastern China. Total fluorescent AGEs level and Nε-carboxymethyl-lysine(CML) content were evaluated by fluorescence spectrophotometry and gas chromatography-mass spectrometry(GC-MS), respectively. The level of total fluorescent AGEs in seafood samples ranged from 39.37 to 1178.3 AU, and was higher in canned and packaged instant aquatic products that were processed at high temperatures. The CML content in seafood samples ranged from 44.8 to 439.1 mg per kg dried sample, and was higher in roasted seafood samples. The total fluorescent AGEs and CML content increased when seafood underwent high-temperature processing, but did not show an obvious correlation. The present study suggested that commonly consumed seafood contains different levels of AGEs, and the seafood processed at high temperatures always displays a high level of either AGEs or CML.     

Glacier changes in the eastern Nyainqêntanglha Range of Tibetan Plateau from 1975 to 2013

Maritime-type glaciers in the eastern Nyainqêntanglha Range, located in the southeastern part of the Tibetan Plateau, are an important water source for downstream residents and ecological systems. To better understand the variability of glaciers in this region, we used the band ratio threshold(TM3/TM5 for the Landsat TM /ETM+ and TM4/TM6 for Landsat OLI) to extract glacier outlines in ~1999 and ~2013. After that, we also generated a series of glacier boundaries and monitored glacier variations in the past 40 years with the help of the Chinese Glacier Inventory data(1975) and Landsat TM, ETM+ and OLI data. The total glacier area decreased by 37.69 ± 2.84% from 1975 to 2013. The annual percentage area change(APAC) was ~1.32% a-1 and ~1.29% a-1 in the periods 1975-1999 and 1999-2013, respectively. According to the lag theory, the reaction time is probably about 10 years and we discuss the variations of temperature and precipitation between 1965 and 2011. Temperature and precipitation increased between 1965 and 2011 at a rate of 0.34°C /10 a and 15.4 mm/10 a, respectively. Extensive meteorological data show that the glacier shrinkage rate over the period may be mainly due to increasing air temperature, while the increasing precipitation partly made up for the mass loss of glacier ice resulting from increasing temperature may also lead to the low APAC between 1999 and 2013. The lag theory suggests that glacier shrinkage may accelerate in the next 10 years. Small glaciers were more sensitive to climate change, and there was a normal distribution between glacier area and elevation. Glaciers shrank in all aspects, and south aspects diminished faster than others.     

Control factors of DIC in the Y3 seamount waters of the Western Pacific Ocean

An investigation was carried out in the Y3 seamount area of the Western Pacific Ocean in December 2014,and the distribution of dissolved inorganic carbon(DIC) and its relationship with environmental factors in this area were explored.The results show that DIC concentration was higher in the adjacent waters of the Y3 seamount area,and the uplift of DIC isolines at the stations was close to the seamount.Meanwhile,interaction between the North Equatorial Current(NEC) and the Y3 seamount affected the DIC distribution i.e.,the upwelling in the same direction of the NEC was obvious,resulting in a decreasing trend of average concentration of DIC in the 200 m water column from the top to the two side s in this direction but in the cross direction.The DIC concentration increased with the water depth increase,and its distribution was affected by various environmental factors.In the surface water,high temperature was a decisive factor for the decrease of the DIC concentration,but the photosynthesis of phytoplankton showing only a weak influence.In the North Pacific Tropic Water(NPTW),DIC production rate from organic matter decomposition was higher than that of DIC consumption by phytoplankton photosynthesis,leading to a continual increase of DIC.In the North Pacific Intermediate Water(NPIW),organic matter decomposition played a leading role in the increase of DIC.In the deep water,decomposition of organic matter weakened,and the dissolution of CaCO_3 controlled the carbonate system,and DIC had the smallest variation range.     

基于生态系统服务价值评估的我国集中连片重点贫困区生态补偿研究

本文基于2010年Landsat TM/ETM+数据,HJ-1卫星CCD数据,MODIS17A3净初级生产力数据和土壤,气候的地面实测数据,采用以生态系统服务价值为基础的区域差异化生态补偿估算方法,对中国14个集中连片贫困区生态补偿标准进行了估算.研究结果表明,2010年中国14个集中连片贫困区生态系统服务总价值达20 627.4亿元,与同年该区域GDP总量 22 096.2亿元大体相当,在南疆三地州,四省藏区和西藏片区3个西部片区,每年的生态系统服务总价值为GDP总量的2-12倍,这对保障中国生态安全具有重要意义.在14个集中连片特困区,生态补偿所需资金每年约为1971.0亿元,平均每县约为2.9亿元.目前,国家给予的生态补偿资金与此相比严重不足,即使是接受一般性财政转移支付的国家重点生态功能区,其各县市平均每年接受补偿资金也仅为0.94亿元,不到所需金额的1/3.生态补偿资金不足不但给各片区扶贫工作带来巨大压力,也带来了严重的生态风险.因此,生态补偿资金增量的投入可能是解决集中连片特困区贫困问题关键所在,在此增量补偿资金基础上,集中连片特困区可建立以国家购买生态产品为主,开发性扶贫增长为辅的可持续经济增长模式,这可能是彻底解决中国集中连片特困地区贫困问题的基本途径.     

面向大区域遥感专题制图的自动化策略

如何消除相邻影像间的信息冗余是实现大区域遥感自动化制图的关键。本文提出了一种基于约束接缝线的区域分块自动化制图方法,首先采用全球通用墨卡托投影-UTM投影的网格带将研究区分为若干小区域;然后,针对每个UTM投影带内的遥感影像,采用影像镶嵌的原理和湖泊边界约束条件计算其接缝线网络,以确定每景遥感数据制图范围;最后,根据其制图范围生成对应的无冗余专题信息,减少计算量和相邻影像重复信息的编辑时间。本文以亚洲中部干旱区为例开展湖泊制图试验,结果证明该方法能够生成顾及湖泊的有效制图范围,提高了遥感大区域制图的效率。     

Protective effects of C-phycocyanin on alcohol-induced acute liver injury in mice

Excessive alcohol consumption leads to liver disease. Extensive evidence suggests that C-phycocyanin (C-PC), a chromophore phycocyanobilin derived from Spirulina platensis, exerts protective effects against chemical-induced organ damage. In this study, we investigated whether C-PC could protect against ethanol-induced acute liver injury. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (CHOL), low-density lipoprotein (LDL), liver homogenate malondialdehyde (MDA), superoxide dismutase (SOD) content were measured, and pathological examination of liver sections were examined. C-PC showed obvious inhibitory effects on serum ALT, AST, TG, CHOL, LDL and MDA, and SOD content significantly increased in the liver. The structure of hepatic lobules was clear, liver sinus returned to normal, and liver cell cords were arranged in neat rows. Cloudiness, swelling, inflammatory cell infiltration and spotty necrosis of liver cells were significantly reduced. Therefore, C-PC can significantly protect against ethanol-induced acute liver injury.     

Antioxidant system responses in two co-occurring green-tide algae under stress conditions

Green tides have occurred every year from 2007 to 2014 in the Yellow Sea. Ulva prolifera(Müller) J. Agardh has been identified as the bloom-forming alga,co-occurring with U. intestinalis. We observed distinct strategies for both algal species during green tides. U. prolifera exhibited a high abundance initially and then decreased dramatically,while U. intestinalis persisted throughout. The antioxidant system responses of these two macroalgae were compared in the late phase of a green tide(in-situ) and after laboratory acclimation. Lipid peroxidation and antioxidant system responses differed significantly between the two. Malondialdehyde and hydrogen peroxide contents increased significantly in-situ in U. prolifera,but not in U. intestinalis. In U. prolifera,we observed a significant decrease in total antioxidant ability(T-AOC),antioxidant enzymes(SOD and Apx),and non-enzyme antioxidants(GSH and As A) in-situ. U. intestinalis showed the same pattern of T-AOC and SOD,but its Gpx,Apx,and GSH responses did not differ significantly. The results suggest that U. prolifera was more susceptible than U. intestinalis to the harsh environmental changes during the late phase of a Yellow Sea green tide. The boom and bust strategy exhibited by U. prolifera and the persistence of U. intestinalis can be explained by differences in enzyme activity and antioxidant systems.     

Two new species of the genus <Emphasis Type="Italic">Cobitis</Emphasis> Linnaeus (Teleostei: Cobitidae) from southern China

Two new species of the genus Cobitis from southern China, C. hereromacula from the Luohe River in Guangdong Province and C. baishagensis from the Nandujiang River in Hainan Province, are described and illustrated here. C. hereromacula can be distinguished from its congeners by possessing the following combination of characteristics: absence of the second and third pigmentary zones of Gambetta; 13–16 oval blotches on the dorsum and 10–13 vertical, elongated triangular blotches below the midlateral line with more than 20 vertical dark brown bars between them; 6–7 narrow rows of dark spots on the caudal fin; a vertical oval spot smaller than the eye diameter on the upper part of the caudal peduncle; pointed mental lobes of the lower lip pointed with a slightly filiform tip; one slender and long needle-shaped lamina circularis at the base of the first branched ray of the male pectoral fins. C. baishagensis can be distinguished from its congeners by the fourth Gambetta zone being covered by 10–12 transverse elongated blotches; 4–5 narrow rows of dark spots on the caudal fin; a vertical blotch smaller than the eye diameter on the upper part of the caudal peduncle; males with a slender and long needle-shaped lamina circularis at the second branched pectoral fin ray in males; large scales with a slightly large focal zone; undeveloped mental lobes with a lower lip that does not end posteriorly in a filiform tip.     

Difference in the influence of Indo-Pacific Ocean heat content on South Asian Summer Monsoon intensity before and after 1976/1977

Monthly ocean temperature from ORAS4 datasets and atmospheric data from NCEP/NCAR Reanalysis I/II were used to analyze the relationship between the intensity of the South Asian summer monsoon(SASM) and upper ocean heat content(HC) in the tropical Indo-Pacific Ocean.The monsoon was differentiated into a Southwest Asian Summer Monsoon(SWASM)(2.5°–20°N,35°–70°E) and Southeast Asian Summer Monsoon(SEASM)(2.5°–20°N,70°–110°E).Results show that before the 1976/77 climate shift,the SWASM was strongly related to HC in the southern Indian Ocean and tropical Pacific Ocean.The southern Indian Ocean affected SWASM by altering the pressure gradient between southern Africa and the northern Indian Ocean and by enhancing the Somali cross-equatorial flow.The tropical Pacific impacted the SWASM through the remote forcing of ENSO.After the 1976/77 shift,there was a close relationship between equatorial central Pacific HC and the SEASM.However,before that shift,their relationship was weak.